Translating Recent Microbiome Insights in Otitis Media into Probiotic Strategies

Broek, Marianne F.L. van den; Boeck, Ilke De; Kiekens, Filip; Boudewyns, An; Vanderveken, Olivier M.; Lebeer, Sarah

doi:10.1128/cmr.00010-18

Cited by 29 publications

(29 citation statements)

References 279 publications

(317 reference statements)

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“…13 New evidence is also identifying several species including some lactic acid bacilli and Dolorsigranulum, that may be protective to the middle ear and may play a role in probiotic development as a novel treatment modality. 14…”

Section: Microbiome Identificationmentioning

confidence: 99%

Review of otitis media microbiome studies: What do they tell us?

Nogues

Pérez‐Losada

Preciado

2020

Laryngoscope Investig Oto

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Objectives: To provide a state of the art review on accruing studies focused on defining the middle ear microbiome, highlighting the relationship of the microbiome to disease pathophysiology. Data sources: Pubmed indexed peer-reviewed articles and published textbooks. Review methods: Comprehensive review of the literature using the following search terms: "microbiome" "bacterial pathogens" with the term "otitis media," and "middle ear." Results: A multitude of microbiome studies have been published in the recent past. In general findings from these studies underscore distinct profiles based on disease category. The adenoidal reservoir theory may not explain all etiologies of middle ear effusion production. The host immune system appears to be associated to the bacterial population identified in the middle ear space. Atopic respiratory diseases correlate to the middle ear microbiome. Some novel middle ear bacterial genera may be protective in terms of disease. Conclusion: The understanding of otitis media disease progression pathophysiology is evolving, informed by accruing middle ear microbiomic data. The functional implications of middle ear microbiome findings need to be studied further. This may help counterbalance probiotic vs antibiotic approaches to disease mitigation.

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Section: Microbiome Identificationmentioning

confidence: 99%

Review of otitis media microbiome studies: What do they tell us?

Nogues

Pérez‐Losada

Preciado

2020

Laryngoscope Investig Oto

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“…Preventing such perturbation or restoring a perturbed microbiota through addition of probiotics, i.e., live microorganisms that, when administered in adequate amounts, confer a health benefit on the host ( 21 ), could be a valuable method for OME prevention and could reduce the need for surgical intervention. Such a probiotic approach is widely used for the gastrointestinal tract but is underexplored for respiratory health or the prevention and treatment of otitis media ( 22 ). The first intervention studies testing local bacteriotherapy to prevent or cure respiratory tract infections used alpha-hemolytic streptococci (AHS) of the mitis and sanguinis groups ( 23 , 24 ), as they are among the first colonizers of the human URT after birth ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Case-Control Microbiome Study of Chronic Otitis Media with Effusion in Children Points at Streptococcus salivarius as a Pathobiont-Inhibiting Species

et al. 2021

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Chronic otitis media with effusion (OME) has been associated with a shift in microbiome composition and microbial interaction in the upper respiratory tract (URT). While most studies have focused on potential pathogens, this study aimed to find bacteria that could be protective against OME through a case-control microbiome study and characterization of isolates from healthy subjects. The URT and ear microbiome profiles of 70 chronic OME patients and 53 controls were compared by 16S rRNA amplicon sequencing. Haemophilus influenzae was the most frequent classic middle ear pathobiont. However, other taxa, especially Alloiococcus otitis, were also frequently detected in the ear canal of OME patients. Streptococci of the salivarius group and Acinetobacter lwoffii were more abundant in the nasopharynx of healthy controls than in OME patients. In addition to the microbiome analysis, 142 taxa were isolated from healthy individuals, and 79 isolates of 13 different Streptococcus species were tested for their pathobiont-inhibiting potential. Of these, Streptococcus salivarius isolates showed a superior capacity to inhibit the growth of H. influenzae, Moraxella catarrhalis, Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, A. otitis, and Corynebacterium otitidis. S. salivarius strains thus show potential as a probiotic for prevention or treatment of OME based on their overrepresentation in the healthy nasopharynx and their ability to inhibit the growth of respiratory pathobionts. (This study has been registered at ClinicalTrials.gov under registration no. NCT03109496.) IMPORTANCE The majority of probiotics marketed today target gastrointestinal health. This study searched for bacteria native to the human upper respiratory tract, with a beneficial potential for respiratory and middle ear health. Comparison of the microbiomes of children with chronic otitis media with effusion (OME) and of healthy controls identified Streptococcus salivarius as a health-associated and prevalent inhabitant of the human nasopharynx. However, beneficial potential should be assessed at strain level. Here, we also isolated specific S. salivarius strains from the healthy individuals in our study. These isolates showed a beneficial safety profile and efficacy potential to inhibit OME pathogens in vitro. These properties will now have to be evaluated and confirmed in human clinical studies.

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“…A recent review by van den Broek et al described novel insights on probiotic therapy in OM [87]. Basing on Koch's postulates, authors introduced the "probiotic postulates" to define the ideal probiotic strain to be used in clinical practice: The microorganism can be found in high abundance in health status and decreased abundance during disease; the microorganism can be isolated from a healthy organism and grown in pure culture; the cultured organism should promote health when introduced into a diseased organism; it should be possible to re-isolate these microorganisms as identical to the original agent from the healthy host.…”

Section: Probiotic Therapymentioning

confidence: 99%

Upper Respiratory Tract Microbiome and Otitis Media Intertalk: Lessons from the Literature

Folino

Ruggiero

Capaccio

et al. 2020

JCM

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Otitis media (OM) is one of the most common diseases occurring during childhood. Microbiological investigations concerning this topic have been primarily focused on the four classical otopathogens (Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pyogenes) mainly because most of the studies have been conducted with culture-dependent methods. In recent years, the introduction of culture-independent techniques has allowed high-throughput investigation of entire bacterial communities, leading to a better comprehension of the role of resident flora in health and disease. The upper respiratory tract (URT) is a region of major interest in otitis media pathogenesis, as it could serve as a source of pathogens for the middle ear (ME). Studies conducted with culture-independent methods in the URT and ME have provided novel insights on the pathogenesis of middle ear diseases through the identification of both possible new causative agents and of potential protective bacteria, showing that imbalances in bacterial communities could influence the natural history of otitis media in children. The aim of this review is to examine available evidence in microbiome research and otitis media in the pediatric age, with a focus on its different phenotypes: acute otitis media, otitis media with effusion and chronic suppurative otitis media.

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Translating Recent Microbiome Insights in Otitis Media into Probiotic Strategies

Cited by 29 publications

References 279 publications

Review of otitis media microbiome studies: What do they tell us?

Review of otitis media microbiome studies: What do they tell us?

Case-Control Microbiome Study of Chronic Otitis Media with Effusion in Children Points at Streptococcus salivarius as a Pathobiont-Inhibiting Species

Upper Respiratory Tract Microbiome and Otitis Media Intertalk: Lessons from the Literature

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