Transient Virus Expression Systems for Recombinant Protein Expression in Dicot- and Monocotyledonous Plants

In Planta Transient Expression Systems for Monocots

“…14.6). In another BSMV vector design, GFP and 42 rice coding gene sequences (CDS), between 200-and 1800-nt in size, were expressed as N-terminal fusions to the haemagglutinin epitope (HA) tag linked to the 2A self-cleaved peptide sequence followed by BSMV γb (Pogue and Holzberg 2012). Western blot analyses of systemically infected barley tissue using anti-HA antibodies confirmed expression of 38 out of 43 recombinant protein products (Fig.…”

“…Apparently, this vector was also capable of expressing proteins requiring maturation through the plant secretory pathway, which demonstrates the ability of the 2A cleavage system to deliver functionally active proteins to distinct subcellular fates. Moreover, the expression was shown to be relatively stable and at least in the case of GFP, its expression was observed regularly in leaves 1-4 above the inoculated leaf and maintained for up to 18 days post inoculation (Pogue and Holzberg 2012).…”

Insect allies—Assessment of a viral approach to plant genome editing

“…A third method for the creation of a transgenic plant is the use of native plant viruses (Komarova et al, 2010; Pogue & Holzberg, 2012). Viruses can be used for virus‐induced gene silencing (VIGS), virus‐mediated gene overexpression (VOX; Bouton et al, 2018; Ramegowda et al, 2014), and for virus‐enabled gene editing (VEdGE; Mei et al, 2019).…”