Transgenic potato lines expressing hairpin RNAi construct of molting-associated EcR gene exhibit enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata, Say)

Hussain, Tahira; Aksoy, Emre; Çalışkan, Mehmet Emin; Bakhsh, Allah

doi:10.1007/s11248-018-0109-7

Cited by 50 publications

(20 citation statements)

References 67 publications

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“…Flight in CPB is also connected with alanine aminotransferase (alt). Hussain et al [73] focused on the suppressed transcripts level of highly expressive Ecdysone receptor (EcR) gene of CPB using plant-mediated RNAi approach. Bioassays of transgenic plants showed 20-80% mortality of CPB instars.…”

Section: Rnai In Colorado Potato Beetle Control Managementmentioning

confidence: 99%

Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

Balaško

Mikac

Bažok

et al. 2020

Insects

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Colorado potato beetle, CPB (Leptinotarsa decemlineata Say), is one of the most important pests of the potato globally. Larvae and adults can cause complete defoliation of potato plant leaves and can lead to a large yield loss. The insect has been successfully suppressed by insecticides; however, over time, has developed resistance to insecticides from various chemical groups, and its once successful control has diminished. The number of available active chemical control substances is decreasing with the process of testing, and registering new products on the market are time-consuming and expensive, with the possibility of resistance ever present. All of these concerns have led to the search for new methods to control CPB and efficient tools to assist with the detection of resistant variants and monitoring of resistant populations. Current strategies that may aid in slowing resistance include gene silencing by RNA interference (RNAi). RNAi, besides providing an efficient tool for gene functional studies, represents a safe, efficient, and eco-friendly strategy for CPB control. Genetically modified (GM) crops that produce the toxins of Bacillus thuringiensis (Bt) have many advantages over agro-technical, mechanical, biological, and chemical measures. However, pest resistance that may occur and public acceptance of GM modified food crops are the main problems associated with Bt crops. Recent developments in the speed, cost, and accuracy of next generation sequencing are revolutionizing the discovery of single nucleotide polymorphisms (SNPs) and field of population genomics. There is a need for effective resistance monitoring programs that are capable of the early detection of resistance and successful implementation of integrated resistance management (IRM). The main focus of this review is on new technologies for CPB control (RNAi) and tools (SNPs) for detection of resistant CPB populations.

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Section: Rnai In Colorado Potato Beetle Control Managementmentioning

confidence: 99%

Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

Balaško

Mikac

Bažok

et al. 2020

Insects

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“…The production of transgenic plants is another strategy to deliver dsRNA for inset pest control. A number of studies have used this approach to develop transgenic plants expressing dsRNA against insects in the order Hemiptera [40][41][42], Coleoptera [17,[43][44][45], or Lepidoptera [16,46,47]. However, transgenic plant-mediated RNAi based on conventional nuclear transformation technology did not offer efficient protection from insect damage, probably due to (i) low expression of dsRNA and (ii) instability of dsRNAs (result from plants' endogenous Dicer cleavage) in planta [14].…”

Section: Discussionmentioning

confidence: 99%

Bacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae)

Zhang

et al. 2019

Insects

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RNA interference (RNAi) has emerged as a novel and feasible strategy for pest management. Methods for cost-effective production and stable delivery of double-stranded RNA (dsRNA) to the target insects are crucial for the wide application of RNAi for pest control. In this study, we tested the expression of dsRNA in RNaseIII-deficient Escherichia coli HT115 which was then fed to Plagiodera versicolora larvae, an insect pest of Salicaceae plants worldwide. By targeting six potential genes, including actin (ACT), signal recognition particle protein 54k (SRP54), heat shock protein 70 (HSC70), shibire (SHI), cactus (CACT), and soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAP), we found that feeding bacteria-expressed dsRNA successfully triggered the silencing of the five target genes tested and the suppression of ACT and SRP54 genes caused significant mortality. Our results suggest that the oral delivery of bacteria-expressed dsRNA is a potential alternative for the control of P. versicolora, and that ACT and SRP54 genes are the potent targets.

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“…Recently, report of transgenic potato lines expressing hairpin RNAi construct of molting-associated EcR gene (associated with highly specific molting) exhibited enhanced resistance against CPB that is notorious insect pests of potato crop worldwide (Figure-3). These promising results reveal the functionality of robustness of RNAi applications for the control of insect pests as effective pest management strategy (Hussain et al, 2019). The nuclease degradation of dsRNA in the gut lumen of Leptinotarsa decemlineata, a CPB, has been thought to be the reason in the responsiveness of orally delivered dsRNA in different insect species.…”

Section: Application Of Recent Technologies Against Biotic Stressmentioning

confidence: 96%

“…The specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to a part or all of a specific gene transcript. These RNAs include micro RNAs and small interfering RNAs, both of which use RNA-induced silencing complexes (RISC) also known as ribonucleoprotein for regulation of target gene repression (Kamthan et al, 2015;Hussain et al, 2019). RNAi has proved its significance in functional genomic research and proved itself as a potential strategy in crop improvement for the control of insect pests and diseases.…”

Section: Application Of Recent Technologies Against Biotic Stressmentioning

confidence: 99%

GENETIC APPROACHES FOR ENGINEERING BIOTIC STRESS RESISTANCE IN POTATO (Solanum tuberosum L.)

Bakhsh¹,

Dangol²,

Naeem³

et al. 2020

THE JAPS

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Potato is one of the most important food crops in terms of annual production and food security worldwide. The crop is affected by several types of biotic stresses, e.g. insects, viruses, fungus, nematodes and weeds, which are the prominent limiting factors for its production. The conventional breeding methods in potato have been associated with limitations; none of the present day commercial cultivar has built-in resistance against biotic stresses. There is strong need for the development of new resistant potato varieties to cope against biotic stresses using non-classical approaches in combination with classical methods. The scientific literature suggests the contribution of modern biotechnological techniques for the development of transgenic potato lines resistant against insects and diseases. The present comprehensive review describes different genetic engineering approaches for the development of transgenic potatoes resistant to insects, weeds, nematodes, fungus and viruses by fellow researchers worldwide. It also gives an insight into modern technologies, e.g. RNAi and CRISPR-Cas9, which have emerged recently and can be implemented in the development of biotic stress resistant potato cultivars.

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Transgenic potato lines expressing hairpin RNAi construct of molting-associated EcR gene exhibit enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata, Say)

Cited by 50 publications

References 67 publications

Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

Bacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae)

GENETIC APPROACHES FOR ENGINEERING BIOTIC STRESS RESISTANCE IN POTATO (Solanum tuberosum L.)

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