Transgenic potato lines expressing CP4-EPSP synthase exhibit resistance against glyphosate

Bakhsh, Allah; Hussain, Tahira; Rahamkulov, Ilhom; Demirel, Ufuk; Çalışkan, Mehmet Emin

doi:10.1007/s11240-019-01708-1

Cited by 15 publications

(18 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overall results showed that using proper hormonal combination in regeneration selection media can lead to increased regeneration frequency of potato cultivars. Using the above-mentioned protocol, insect- and herbicide-resistant potato lines expressing cry1Ac gene ( 27 ), cp4-epsps ( 28 ) and hairpin construct of insect metamorphosis-associated gene ( 29 ) were developed.…”

Section: Resultsmentioning

confidence: 99%

Development of Efficient, Reproducible and Stable Agrobacterium-Mediated Genetic Transformation of Five Potato Cultivars

Bakhsh

2020

Food technol. biotechnol. (Online)

Self Cite

View full text Add to dashboard Cite

The developments in transformation technology have enabled the scientists to incorporate, mutate or substitute gene(s) leading to a particular trait; advancing it to a point where only few technical limitations remain. Genotype dependency and explant types are important factors affecting transformation efficiency in potato. In the present study, a rapid, reproducible and stable Agrobacterium-mediated transformation procedure in potato was developed by a combination of different plant growth regulators. Leaf discs and internodal explants of five cultivars of potato, i.e. Lady Olympia, Granola, Agria, Désirée and Innovator were infected with Agrobacterium tumefaciens strain LBA4404 containing pBIN19 expression vector with β-glucuronidase gusA gene under the control of 35S CaMV promoter. Kanamycin was used as plant selectable marker for screening of primary transformants at concentration of 100 mg/L. Both explants responded positively; internode being more suitable explant for better transformation efficiency. Based on GUS histochemical assay, the transformation efficiency was 22, 20, 18.6, 15 and 10 % using the internodal explant, and 15, 12, 17, 8 and 6 % using leaf discs as explant in Lady Olympia, Granola, Agria, Désirée and Innovator respectively. Furthermore, PCR assays confirmed the presence of gusA and nptII genes in regenerated plants. The molecular analysis in succeeding progeny showed proper integration and expression of both genes. The results suggest Lady Olympia as the best cultivar for future transformation procedures. Overall, the short duration, rapidity and reproducibility make this protocol suitable for wider application of transgenic potato plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Development of Efficient, Reproducible and Stable Agrobacterium-Mediated Genetic Transformation of Five Potato Cultivars

Bakhsh

2020

Food technol. biotechnol. (Online)

Self Cite

View full text Add to dashboard Cite

show abstract

“…Agrobacterium-mediated transformation of both cultivars was performed as described by Bakhsh et al (2020) with minor modifications. Since the main objective of the study was to determine the role of stu-miR172b-3p in potato during heat and drought stresses, the transgenic plants of both drought-tolerant (Unica) and sensitive (Russet Burbank) cultivars were obtained and confirmed by PCR analysis (Figure S1).…”

Section: Resultsmentioning

confidence: 99%

“…The leaf and internodal explants of both cultivars were inoculated with Agrobacterium suspension (A600 nm = 0.7) for 10 min with gentle shaking in a liquid medium without antibiotics followed by incubation on co-cultivation medium for approximately three days. Overall, the transformation procedure was followed as described by Bakhsh et al (2020) with some modifications.…”

Section: Construction Of Transformation Vector and Production Of Mir172b-3p Overexpressing Transgenic Linesmentioning

confidence: 99%

Individual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p

Asim¹,

Gökçe²,

Bakhsh³

et al. 2021

Turk J Agric For

Self Cite

View full text Add to dashboard Cite

efficiency. Meanwhile, the accumulation of hydrogen peroxide (H 2 O 2 ) was reduced in both cultivars, proving that it is involved in the front-line tolerance mechanism against individual drought, heat, and their combination. In conclusion, our results prove that the stress tolerance could be enhanced by miR172b-3p-mediated negative regulation of ERTF RAP2-7-like gene in potato under drought, heat, and their combination. Our findings represent the first step towards the improvement of tolerance against multiple abiotic stresses in potato.

show abstract

“…However, with the widespread approval and adoption of other biotech crops, there is a renewed interest in the development of biotech potato which has led to the arrival of biotech potatoes back in the market in 2015. Very recently, Bakhsh et al (2020) engineered herbicide resistant trait in potatoes by incorporating CP-4 EPSP synthase gene in different cultivars i.e. Lady Olympia, Desiree, Agria and Granola.…”

Section: Herbicide Resistant Potatoesmentioning

confidence: 99%

GENETIC APPROACHES FOR ENGINEERING BIOTIC STRESS RESISTANCE IN POTATO (Solanum tuberosum L.)

Bakhsh¹,

Dangol²,

Naeem³

et al. 2020

THE JAPS

View full text Add to dashboard Cite

Potato is one of the most important food crops in terms of annual production and food security worldwide. The crop is affected by several types of biotic stresses, e.g. insects, viruses, fungus, nematodes and weeds, which are the prominent limiting factors for its production. The conventional breeding methods in potato have been associated with limitations; none of the present day commercial cultivar has built-in resistance against biotic stresses. There is strong need for the development of new resistant potato varieties to cope against biotic stresses using non-classical approaches in combination with classical methods. The scientific literature suggests the contribution of modern biotechnological techniques for the development of transgenic potato lines resistant against insects and diseases. The present comprehensive review describes different genetic engineering approaches for the development of transgenic potatoes resistant to insects, weeds, nematodes, fungus and viruses by fellow researchers worldwide. It also gives an insight into modern technologies, e.g. RNAi and CRISPR-Cas9, which have emerged recently and can be implemented in the development of biotic stress resistant potato cultivars.

show abstract

Transgenic potato lines expressing CP4-EPSP synthase exhibit resistance against glyphosate

Cited by 15 publications

References 39 publications

Development of Efficient, Reproducible and Stable Agrobacterium-Mediated Genetic Transformation of Five Potato Cultivars

Development of Efficient, Reproducible and Stable Agrobacterium-Mediated Genetic Transformation of Five Potato Cultivars

Individual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p

GENETIC APPROACHES FOR ENGINEERING BIOTIC STRESS RESISTANCE IN POTATO (Solanum tuberosum L.)

Contact Info

Product

Resources

About