Transgenic ipt tobacco overproducing cytokinins overaccumulates phenolic compounds during in vitro growth

Schnablová, Renáta; Synková, Helena; Vičánková, A.; Burketová, Lenka; Eder, J.; Cvikrová, Milena

doi:10.1016/j.plaphy.2006.09.004

Cited by 46 publications

(26 citation statements)

References 30 publications

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“…However, in our experiments, there was no addition of exogenous phenolic compounds to the culture medium, suggesting the occurrence of polyphenols by the synthesis of new shoot tips after their establishment in vitro in response to new growing conditions (Alemanno et al 2003). These phenolic compounds may be responsible for the increase in the endogenous auxin levels due to its role as an IAA (indole-3-acetic acid) oxidize inhibitor (Wilson and Van Staden 1990;Hausman 1993) and due to the modulation of the endogenous levels of IAA (Schnablová et al 2006;Reis et al 2008), thus enhancing the role of the auxin applied to the medium culture of shoot apexes. In addition, the role of phenolic compounds appears to be most likely related to the cellular dedifferentiation process (Alemanno et al 2003) and the early stages of somatic embryo differentiation (Reis et al 2008).…”

Section: Multicellular Embryogenesis and Organogenesismentioning

confidence: 68%

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Almeida

Almeida²,

Graner

et al. 2012

Plant Cell Rep

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Section: Multicellular Embryogenesis and Organogenesismentioning

confidence: 68%

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Almeida

Almeida²,

Graner

et al. 2012

Plant Cell Rep

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“…This discrepancy with our findings may originate from the fact that an artificial tissue culture system was used by those authors. In the other case, the levels of phenolic compounds, including the scopoletin precursor p-coumaric acid, were increased in CK-overproducing ipt transgenic tobacco plants, whereas SA levels were reduced (Schnablová et al, 2006).…”

Section: Ck-induced Phytoalexin Production Causes Pathogen Resistancementioning

confidence: 94%

Cytokinins Mediate Resistance againstPseudomonas syringaein Tobacco through Increased Antimicrobial Phytoalexin Synthesis Independent of Salicylic Acid Signaling

Großkinsky

Naseem²,

Abdelmohsen³

et al. 2011

Plant Physiology

183

169

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Cytokinins are phytohormones that are involved in various regulatory processes throughout plant development, but they are also produced by pathogens and known to modulate plant immunity. A novel transgenic approach enabling autoregulated cytokinin synthesis in response to pathogen infection showed that cytokinins mediate enhanced resistance against the virulent hemibiotrophic pathogen Pseudomonas syringae pv tabaci. This was confirmed by two additional independent transgenic approaches to increase endogenous cytokinin production and by exogenous supply of adenine-and phenylurea-derived cytokinins. The cytokinin-mediated resistance strongly correlated with an increased level of bactericidal activities and up-regulated synthesis of the two major antimicrobial phytoalexins in tobacco (Nicotiana tabacum), scopoletin and capsidiol. The key role of these phytoalexins in the underlying mechanism was functionally proven by the finding that scopoletin and capsidiol substitute in planta for the cytokinin signal: phytoalexin pretreatment increased resistance against P. syringae. In contrast to a cytokinin defense mechanism in Arabidopsis (Arabidopsis thaliana) based on salicylic acid-dependent transcriptional control, the cytokininmediated resistance in tobacco is essentially independent from salicylic acid and differs in pathogen specificity. It is also independent of jasmonate levels, reactive oxygen species, and high sugar resistance. The novel function of cytokinins in the primary defense response of solanaceous plant species is rather mediated through a high phytoalexin-pathogen ratio in the early phase of infection, which efficiently restricts pathogen growth. The implications of this mechanism for the coevolution of host plants and cytokinin-producing pathogens and the practical application in agriculture are discussed.

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“…While INCYDE significantly increased the quantity of the phenolics in 75 mM NaCl-stressed plants, a drastic lower concentration of phenolic was prominent at higher concentration of NaCl as well as in the non-stressed tomato plants. The role of CKs and/or INC-YDE on phenolic acid biosynthetic pathway has been hypothesized (Schnablová et al 2006;Aremu et al 2012a). Despite this assertion, the non-availability of the quality of phenolic compounds produced in the current study implies that the specific role of INCYDE on phenolic production during salinity stress remains speculative and obscure.…”

Section: Effect Of Incyde Treatment On Mda Concentration Phenolic Comentioning

confidence: 72%

A novel inhibitor of cytokinin degradation (INCYDE) influences the biochemical parameters and photosynthetic apparatus in NaCl-stressed tomato plants

Aremu

Sunmonu

Kulkarni

et al. 2014

Planta

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The effect of 2-chloro-6-(3-methoxyphenyl)aminopurine [inhibitor of cytokinin degradation (INCYDE)] at 10 nM on growth, biochemical and photosynthetic efficiency in sodium chloride (NaCl)-stressed (75, 100 and 150 mM) tomato plants was investigated. NaCl-induced decline in plant vigor index was slightly reversed by both drenching and foliar application of INCYDE. Foliar application of INCYDE significantly increased the flower number in the control and 75 mM NaCl-supplemented plants, while drenching was more effective in 150 mM NaCl-stressed plants. Antioxidant enzymes (peroxidase, catalase and superoxide dismutase) were enhanced in the presence of INCYDE in the control and NaCl-stressed plants. Higher concentration of malondialdehyde (MDA) associated with oxidative (lipid peroxidation) damage in leaf tissue which was evident in the presence of NaCl stress was significantly attenuated with the drenching and foliar application of INCYDE. Regardless of NaCl concentration, application of INCYDE had no significant influence on maximum quantum efficiency of photosystem II. However, the reduced quantum yield of photosystem II and coefficient of photochemical quenching under continuous illumination with actinic light at four intensities (264, 488, 800 and 1,200 µmol m(-2) s(-1)) in NaCl-stressed (100 and 150 mM) tomato plants were significantly alleviated by drenching application with INCYDE. Non-photochemical quenching of the singlet excited state of chlorophyll a and relative electron transfer rate were generally higher in INCYDE-treated plants than in the controls. From an agricultural perspective, these findings indicate the potential of INCYDE in protecting plants against NaCl stress and the possibility of enhanced productivity.

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Transgenic ipt tobacco overproducing cytokinins overaccumulates phenolic compounds during in vitro growth

Cited by 46 publications

References 30 publications

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Cytokinins Mediate Resistance againstPseudomonas syringaein Tobacco through Increased Antimicrobial Phytoalexin Synthesis Independent of Salicylic Acid Signaling

A novel inhibitor of cytokinin degradation (INCYDE) influences the biochemical parameters and photosynthetic apparatus in NaCl-stressed tomato plants

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