Transgenic barley: A prospective tool for biotechnology and agriculture

Mrízová, Katarína; Holásková, Edita; Öz, Mehmet Tufan; Jiskrová, Eva; Frébort, Ivo; Galuszka, Petr

doi:10.1016/j.biotechadv.2013.09.011

Cited by 44 publications

(25 citation statements)

References 305 publications

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“…The efficiency of transformation also depends on the ability of selection procedure and the frequency of shoot regeneration and (pollen or somatic) embryogenesis (Sobhanian et al 2012). The difficulties in DNA delivery as well as regeneration of the target plant species are the main challenges for genetic transformation of cereals and monocotyledonous plants using routine methods of gene transformation (Mrízová et al 2014). There are some plant gene transformation methods that are independent of tissue culture procedure and can facilitate gene transformation in plants that do not have developed plant tissue culture protocol.…”

Section: Reviewmentioning

confidence: 99%

Tissue culture-based Agrobacterium-mediated and in planta transformation methods

Niazian¹,

Noori²,

Galuszka³

et al. 2017

Czech J. Genet. Plant Breed.

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Niazian M., Sadat Noori S.A., Galuszka P., Mortazavian S.M.M. (2017): Tissue culture-based Agrobacterium-mediated and in planta transformation methods. Czech J. Genet. Plant Breed., 53: 133−143.Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is the Agrobacterium-mediated method. The main problem of this method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. The development of a genotype-independent gene transformation method is of great interest in many plants. Some Agrobacteriummediated gene transformation methods independent of tissue culture are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than transformation methods based on tissue culture. Vacuum infiltration, injection of Agrobacterium culture into plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much faster than regular Agrobacterium-mediated gene transformation based on tissue culture and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular Agrobacterium-mediated transformation methods based on tissue culture. Finally, successful recent transformations using these methods are presented.

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Section: Reviewmentioning

confidence: 99%

Tissue culture-based Agrobacterium-mediated and in planta transformation methods

Niazian¹,

Noori²,

Galuszka³

et al. 2017

Czech J. Genet. Plant Breed.

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“…Barley is considered a model species for the Triticeae cereals as it is a well-characterized diploid cereal with the genome almost fully sequenced (The International Barley Genome Sequencing Consortium 2012). Barley also has a number of molecular and genetic techniques are available, including a relatively efficient Agrobacterium-mediated transformation system (Mrizova et al, 2014). Particularly, barley has the advantage in biosafety aspects of being a self-pollinating species with for many cultivars; a closed type of flowering and outcrossings with other barley plants or related species are extremely rare (Ritala et al, 2002).…”

Section: Ftu/kgmentioning

confidence: 99%

Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains

Holme

Dionisio

Madsen

et al. 2016

Plant Biotechnology Journal

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SummaryThe phytase purple acid phosphatase (HvPAPhy_a) expressed during barley seed development was evaluated as transgene for overexpression in barley. The phytase was expressed constitutively driven by the cauliflower mosaic virus 35S‐promoter, and the phytase activity was measured in the mature grains, the green leaves and in the dry mature vegetative plant parts left after harvest of the grains. The T2‐generation of HvPAPhy_a transformed barley showed phytase activity increases up to 19‐fold (29 000 phytase units (FTU) per kg in mature grains). Moreover, also in green leaves and mature dry straw, phytase activities were increased significantly by 110‐fold (52 000 FTU/kg) and 57‐fold (51 000 FTU/kg), respectively. The HvPAPhy_a‐transformed barley plants with high phytase activities possess triple potential utilities for the improvement of phosphate bioavailability. First of all, the utilization of the mature grains as feed to increase the release of bio‐available phosphate and minerals bound to the phytate of the grains; secondly, the utilization of the powdered straw either directly or phytase extracted hereof as a supplement to high phytate feed or food; and finally, the use of the stubble to be ploughed into the soil for mobilizing phytate‐bound phosphate for plant growth.

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“…The primary application of genome-editing tools is obtaining knockout plants, and in time, the other applications that extend to crop improvement are expected, e.g., abiotic stress tolerance will be important for near future to resolve stress response and adaptation pathways [43]. For example, barley has been used for a long time in genomics studies as a highly adaptive and tolerant model for environmental stresses [44,45]. [46], whereas gene replacement by HR in plants may be increased to 10 −2 through transient expression of meganucleases, which induces double-strand breaks (DSBs) [2].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Plant Genome Editing and its Applications in Cereals

Weinthal¹,

Gürel²

2016

Genetic Engineering - An Insight Into the Strategies and Applications

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Recently developed methods for genome editing, representing a major breakthrough in the ield of genetic engineering, will enable researchers to produce transgenic plants in a more convenient and safer way. Double-strand breaks (DSBs) are triggered by synthetic nucleases that later induce DNA repair mechanisms known as nonhomologous-end joining (NHEJ) or homology-directed repair (HDR) in the presence of a donor DNA. Gene targeting (GT) was earlier demonstrated in rice and maize genomes by exploiting several genes (Acetohydroxyacid synthase, waxy, ALS, OS11N3 etc.), while zinc inger nucleases (ZFNs) were used to modify IPK1 gene in maize. Clustered regularly interspaced short palindromic repeats (CRISPR-CAS) system has been shown to be eicient for targeted mutagenesis in wheat that has a hexaploid complex genome, rice, maize, and recently in barley. The CRISPR system is considered as advantageous over previous approaches due to its easy use and eiciency, however, needs to be improved for high of-target efects.

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Transgenic barley: A prospective tool for biotechnology and agriculture

Cited by 44 publications

References 305 publications

Tissue culture-based Agrobacterium-mediated and in planta transformation methods

Tissue culture-based Agrobacterium-mediated and in planta transformation methods

Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains

Plant Genome Editing and its Applications in Cereals

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