Transforming growth factor β mRNA and protein expression in the ovary of the chicken embryo

Abstract

The expression pattern of transforming growth factor beta (TGFβ) isoforms in chicken embryo gonads was studied at 6-10 days of incubation. TGFβ2 mRNA was expressed predominantly in the cortex of the left ovary from day 8 of incubation onwards. TGFβ3 mRNA was not detected at any of the stages studied. Similarly, immunofluorescence for the TGFβ protein revealed that at day 9 it was located throughout the cortex of the left ovary and in the medulla of both the left and right ovaries. The presence of phosphorylated Smad2 in the nuclei of these regions suggests that TGFβ signaling is most likely active at this developmental stage. Culturing the left ovary in a TGFβ1-supplemented medium induced a shift of cortical structures toward the medulla, suggesting a role for TGFβ in the morphogenesis of the female gonad in chickens.

Various LED Wavelengths Affected Myofiber Development and Satellite Cell Proliferation of Chick Embryos via the IGF‐1 Signaling Pathway

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Research Progress on MicroRNAs Involved in the Regulation of Chicken Diseases

“…Later, using the same chicken lines, Truong and colleagues explored the effects of TGF-β and miRNAs on NE. Members of the TGF-β family that is composed of TGF-β, BMPs, and SMADs function in the immune response (Escalona et al, 2012). According to their results, TGF-β1-3, BMP1-7, and SMAD1-9 were differentially expressed between the two chicken lines relative to their respective controls.…”