In ovo exposure to monochromatic lights affect posthatch muscle growth and satellite cell proliferation of chicks: role of IGF-1

Abstract

To study the role of IGF-1 on stimulation with monochromatic light during incubation altering posthatch muscle growth, chicken embryos were exposed to blue light, green light, red light, white light or darkness throughout embryonic period and then were raised in white light conditions upon hatching. Comparing with the other treatment groups, the chicks in green light group had heavier hatching weights, higher muscle indexes and larger muscle fibers. Both in vivo and in vitro studies showed that the number and proliferative activity of satellite cells in green light group were the highest. Plasma IGF-1 level and skeletal muscle IGF-1R mRNA level were higher in green light group. Moreover, exogenous IGF-1 increased the proliferative activity of satellite cell in a dose-dependent fashion. These results suggest that stimulation with monochromatic green light during incubation promoted posthatch muscle growth and satellite cell proliferation of chicks through IGF-1 signaling.

Various LED Wavelengths Affected Myofiber Development and Satellite Cell Proliferation of Chick Embryos via the IGF‐1 Signaling Pathway

“…Satellite cells were harvested using a previously published method ; the specific steps are as follows: The eggshell was sterilized with 75% alcohol; the pectoral and gastrocnemius muscles were removed from the embryo under sterile conditions using petri dishes; and the fat, fascia and connective tissue were carefully removed. Muscles were washed with pH 7.4 Hanks balanced salt solution, cut into approximately 1‐mm pieces and then treated with 0.1% collagenase type I (1 g L −1 ; Gibico BRL, Grand Island, NY) at 37°C for 30 min and 0.25% trypsin (Gibico BRL) solution at 37°C for 20 min.…”

“…After culturing satellite cells for 24 h, we discarded the supernatant, and the cells were washed two times with DMEM. Then, different concentrations of IGF‐1 (Final concentration: 0, 50, 100 and 200 ng mL −1 ; Sigma) were added to the cell culture media, with DMEM in the control wells, as previously published . Satellite cell activity was detected using the MTT assay as described above.…”

“…Because the development of chicken embryos is independent of the matrix, this characteristic provides convenience to study the effect of photobiomodulation on skeletal muscle development. Previous studies have demonstrated that light stimulation during incubation affects muscle growth and satellite cell proliferation in young chicks , but the effects and the mechanisms of satellite cell proliferation and differentiation by monochromatic light stimulation during embryogenesis remain unclear.…”

Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling Pathway

“…Furthermore, green light (GL) stimulation has been shown to accelerate muscle growth in broilers [4], and enhance the secretion of insulin-like growth factor I (IGF-I) by chick embryo hepatocytes [5,6]. IGF-I has been shown to be a key regulator of muscle development and metabolism in birds and other vertebrate species [7,8], and promoted satellite cells proliferation through IGF-I signaling [9]. Liu et al [10] have shown that GL illumination promoted chick satellite cell myogenic processes during early posthatch stages, and IGF-I played a central role.…”

Role of Melatonin in Monochromatic Light Affecting the Expression of Somatostatin in Hypothalamus and Pituitary of Chicks

“…Then light information is converted into the biological signals, which affects the secretion of plasma hormones through the pituitary portal system and regulates the physiological function of the body [3]. Previous studies have found that green light signi cantly elevated liver insulin growth factor-1 (IGF-1) secretion [4], skeletal satellite cells proliferation [5,6], muscle growth [7,8], and improved meat quality in broilers [9,10]. While, the growth hormone [11] plays an important role in promoting the growth and development and improving the productive performance of broilers [12].…”