Transforming growth factor beta inhibits proliferation of somatic cells without influencing germ cell number in the chicken embryonic ovary

Méndez, C.; Alcántara, Luz; Escalona, René; López‐Casillas, Fernando; Pedernera, E.

doi:10.1007/s00441-005-0145-5

Cited by 9 publications

(7 citation statements)

References 29 publications

(36 reference statements)

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“…Previous studies have demonstrated that in organ culture both TGFb1 and TGFb2 reduced the number of ovarian somatic cells without affecting the number of germ cells. This effect was due to a reduction in cell proliferation rather than increasing apoptosis, assessed by BrdU incorporation and TUNEL, respectively (Méndez et al 2006). The inhibitory effect of TGFb2 over somatic cells in the chicken embryo ovary has also been reported by Fujioka et al (2004).…”

Section: Discussionmentioning

confidence: 83%

“…TGFb2 is prominently expressed in the embryonic gonad of both sexes (Hattori et al 2002). Moreover, it has been demonstrated that in cultured ovaries of E9 chicken embryos, TGFb1 and TGFb2 reduce the number of somatic cells without affecting the number of germ cells (Méndez et al 2006). There is little evidence for TGFb being involved in the embryonic development of the avian gonad.…”

Section: Introductionmentioning

confidence: 97%

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Transforming growth factor β mRNA and protein expression in the ovary of the chicken embryo

et al. 2012

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The expression pattern of transforming growth factor beta (TGFβ) isoforms in chicken embryo gonads was studied at 6-10 days of incubation. TGFβ2 mRNA was expressed predominantly in the cortex of the left ovary from day 8 of incubation onwards. TGFβ3 mRNA was not detected at any of the stages studied. Similarly, immunofluorescence for the TGFβ protein revealed that at day 9 it was located throughout the cortex of the left ovary and in the medulla of both the left and right ovaries. The presence of phosphorylated Smad2 in the nuclei of these regions suggests that TGFβ signaling is most likely active at this developmental stage. Culturing the left ovary in a TGFβ1-supplemented medium induced a shift of cortical structures toward the medulla, suggesting a role for TGFβ in the morphogenesis of the female gonad in chickens.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 97%

Transforming growth factor β mRNA and protein expression in the ovary of the chicken embryo

et al. 2012

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“…TGIF1 is a TGFβ signaling inhibitor, binding to phospho-Smad2, recruiting histone deacetylases and acting as a co-repressor of Smad target genes ( Wotton et al, 1999a ). Chicken ovaries exposed to TGFβ1 display a reduction of somatic cells due to decreased cell proliferation ( Méndez et al, 2006 ). In addition, there is a reduction in the number of germ cells in the cortex and an increased number in the medulla ( Méndez et al, 2006 ).This suggests an effect in the cortical compartment or in the capacity of germ cells to migrate.…”

Section: Discussionmentioning

confidence: 99%

The homeobox gene TGIF1 is required for chicken ovarian cortical development and generation of the juxtacortical medulla

et al. 2021

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During early embryogenesis in amniotic vertebrates, the gonads differentiate into either ovaries or testes. The first cell lineage to differentiate gives rise to the supporting cells; Sertoli cells in males and pre-granulosa cells in females. These key cell types direct the differentiation of the other cell types in the gonad, including steroidogenic cells. The gonadal surface epithelium and the interstitial cell populations are less well studied, and little is known about their sexual differentiation programs. Here, we show the requirement of the homeobox transcription factor gene TGIF1 for ovarian development in the chicken embryo. TGIF1 is expressed in the two principal ovarian somatic cell populations, the cortex and the pre-granulosa cells of the medulla. TGIF1 expression is associated with an ovarian phenotype in estrogen-mediated sex reversal experiments. Targeted mis-expression and gene knockdown indicate that TGIF1 is required, but not sufficient, for proper ovarian cortex formation. In addition, TGIF1 is identified as the first known regulator of juxtacortical medulla development. These findings provide new insights into chicken ovarian differentiation and development, specifically cortical and juxtacortical medulla formation.

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“…TGIF1 is a TGF-b signaling inhibitor, binding to phospho-Smad2, recruiting histone deacetylases and acting as a co-repressor of Smad target genes (Wotton et al, 1999a). Chicken ovaries exposed to TGF-b1 display a reduction of somatic cells due to decreased cell proliferation (Mendez et al, 2006). In addition, there is a reduction in the number of germ cells in the cortex and an increased number in the medulla (Mendez et al, 2006).This suggests an effect in the cortical compartment or in the capacity of germ cells to migrate.…”

Section: Discussionmentioning

confidence: 99%

“…Chicken ovaries exposed to TGF-b1 display a reduction of somatic cells due to decreased cell proliferation (Mendez et al, 2006). In addition, there is a reduction in the number of germ cells in the cortex and an increased number in the medulla (Mendez et al, 2006).This suggests an effect in the cortical compartment or in the capacity of germ cells to migrate. In mice, nodal, activin and TGF-b signalize through Smad 2/3/4 and are key in testicular development, suppressing the pre-granulosa program (Gustin et al, 2016, Wu et al, 2013.…”

Section: Discussionmentioning

confidence: 99%

TGIF1is required for chicken ovarian cortical development and generation of the juxtacortical medulla

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Major

et al. 2021

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During early embryogenesis in amniotic vertebrates, the gonads differentiate into either ovaries or testes. The first cell lineage to differentiate gives rise to the supporting cells; Sertoli cells in males and pre-granulosa cells in females. These key cell types direct the differentiation of the other cell types in the gonad, including steroidogenic cells. The gonadal surface epithelium and the interstitial cell populations are less well studied, and little is known about their sexual differentiation programs. Here, we show the requirement of the transcription factor gene TGIF1 for ovarian development in the chicken embryo. TGIF1 is expressed in the two principal ovarian somatic cell populations, the cortex and the pre-granulosa cells of the medulla. TGIF1 expression is associated with an ovarian phenotype in sex reversal experiments. In addition, targeted over-expression and gene knockdown experiments indicate that TGIF1 is required for proper ovarian cortical formation. TGIF1 is identified as the first known regulator of juxtacortical medulla formation. These findings provide new insights into chicken ovarian differentiation and development, specifically in the process of cortical and juxtacortical medulla formation, a poorly understood area.

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Transforming growth factor beta inhibits proliferation of somatic cells without influencing germ cell number in the chicken embryonic ovary

Cited by 9 publications

References 29 publications

Transforming growth factor β mRNA and protein expression in the ovary of the chicken embryo

Transforming growth factor β mRNA and protein expression in the ovary of the chicken embryo

The homeobox gene TGIF1 is required for chicken ovarian cortical development and generation of the juxtacortical medulla

TGIF1is required for chicken ovarian cortical development and generation of the juxtacortical medulla

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