The homeobox gene <i>TGIF1</i> is required for chicken ovarian cortical development and generation of the juxtacortical medulla

Estermann, Martín; Hirst, Claire Elizabeth; Major, Andrew T.; Smith, Craig A.

doi:10.1242/dev.199646

Cited by 12 publications

(26 citation statements)

References 116 publications

(201 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To evaluate the role of TOX3 on testicular differentiation two different TOX3 shRNAs ( sh370 and sh685 ) were cloned into RCASBP(A) viral vector expressing BFP reporter and the shRNA (37). To test the ability of the shRNA to knock down TOX3 expression, DF-1 cells were transfected with RCASBP plasmids expressing one or other of these shRNAs.…”

Section: Resultsmentioning

confidence: 99%

“…To test the ability of the shRNA to knock down TOX3 expression, DF-1 cells were transfected with RCASBP plasmids expressing one or other of these shRNAs. As a control, DF-1 cells were transfected with a non-silencing shRNA (37). After all cells had become BFP positive, they were transfected with RCASBP(D)-GFP-T2A-TOX3 over-expression plasmid.…”

Section: Tox3 Knock Down Results In Increased Leydig Cell Differentia...mentioning

confidence: 99%

“…Two different shRNAs ( sh370 and sh685 ) were designed against TOX3 ORF, ranked for effectiveness (75) and cloned into RCASBP(A)-BFP plasmid (37). Correct cloning and sequence were confirmed by Sanger sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…Sex reversal experiments were performed as described previously (37). Briefly E3.5 (HH stage 19) eggs were injected with 1.0 mg of Fadrozole (Novartis), PBS (Vehicle), 0.1mg of E2 in 10% Ethanol in Sesame oil solution or vehicle (Control).…”

Section: Sex Reversal Experimentsmentioning

confidence: 99%

See 3 more Smart Citations

DMRT1 regulation ofTOX3modulates expansion of the gonadal steroidogenic cell lineage

Estermann

Major

Smith

2022

Preprint

View full text Add to dashboard Cite

Vertebrate gonads comprise three primary cell types, germ cells, steroidogenic cells, and supporting cells. The latter are the first cell type to differentiate in the embryonic gonad and direct the formation of other somatic lineages. During gonadal sex determination, the supporting cell lineage differentiates into Sertoli cells in males and pre-granulosa cells in females. In the chicken embryo, the molecular trigger for Sertoli cell differentiation is the Z-linked gene DMRT1. Recently, single cell RNA-seq data indicate that that chicken steroidogenic cells, derive from differentiated supporting cells. This differentiation process is achieved by a sequential upregulation of steroidogenic genes and down-regulation of supporting cell markers. The exact mechanism regulating this differentiation process remains unknown. We identified the gene TOX3 as a novel transcription factor expressed in embryonic Sertoli cells of the chicken testis. TOX3 knockdown in males resulted in increased CYP17A1 positive Leydig cells. TOX3 over-expression in male and female gonads resulted in a significant decline in CYP17A1 positive steroidogenic cells. TOX3 expression is negatively regulated by estrogens in vivo, but not induced during masculinization induced by estrogen inhibition. In ovo knock-down of the testis determinant, DMRT1, in male gonads resulted in a down-regulation of TOX3 expression. Conversely, DMRT1 over-expression caused an increase in TOX3 expression. Taken together, this data indicates that DMRT1 regulation of TOX3 modulates expansion of the steroidogenic lineage, either directly, via cell lineage allocation, or indirectly via signaling from the supporting to steroidogenic cell populations.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Tox3 Knock Down Results In Increased Leydig Cell Differentia...mentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Sex Reversal Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

DMRT1 regulation ofTOX3modulates expansion of the gonadal steroidogenic cell lineage

Estermann

Major

Smith

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In birds, exogenous modulation of estrogen levels can influence the embryonic gonadal fate, independent of the genetic sex. Injection of fadrozole, an estrogen synthesis inhibitor, in female embryos results in testicular development, upregulating DMRT1 and AMH and downregulating FOXL2 and aromatase (29, 34, 38, 53). In some cases, these gonads reverted to ovotestis, upregulating aromatase at around day 7 to 9 of development (32, 38, 54).…”

Section: Discussionmentioning

confidence: 99%

Fadrozole-mediated sex reversal in the embryonic chicken gonad involves a PAX2 positive undifferentiated supporting cell state

Estermann

Smith

2022

Preprint

Self Cite

View full text Add to dashboard Cite

Gonadal sex differentiation among vertebrates involves divergent fates of a common groups of progenitor cells present in both presumptive ovaries and testes. The first cell type to differentiate gives rise to pre-Sertoli cells in the testis, and pre-follicular cells in the ovary. These cells derive form a common lineage of so-called supporting cells. In birds and other egg-laying vertebrates, locally synthesised estrogen has a central role in ovarian development and influence the fate of these supporting cells. Manipulation of estrogen levels during embryonic development induces gonadal sex reversal, providing an experimental setting to evaluate the process of gonadal sex differentiation. Recently, we identified PAX2 as a novel marker of the undifferentiated supporting cell lineage in the chicken embryo, expressed in both sexes prior to overt gonadal sex differentiation. PAX2 expression is downregulated at the onset of gonadal sex differentiation in both males and females. The analysis of this undifferentiated supporting cell marker, together with Sertoli (male) and pre-granulosa (female) will enhance our understanding of supporting cell differentiation. Here we characterized the supporting cells differentiation process and identified undifferentiated supporting cells in estrogen-mediated sex reversal experiments. Female embryos treated with the aromatase inhibitor fadrozole developed ovotestis, containing pre-granulosa cells, Sertoli cells and PAX2 positive undifferentiated supporting cells. In contrast, male embryos treated with estradiol showed no PAX2+ undifferentiated gonadal supporting cells. Fadrozole time-course as well as multiple dose analysis suggests that supporting cell transdifferentiation involves a dedifferentiation event into a PAX2+ undifferentiated supporting cell state, followed by a redifferentiation towards the opposite sex lineage.

show abstract

Quantitative proteomics analysis of chicken embryos reveals key proteins that affect right gonadal degeneration in females

et al. 2023

View full text Add to dashboard Cite

In birds, embryonic gonads of females develop in a way different from mammals, with the left one develops into a functional ovary, while the right one degenerates during embryogenesis. Here, we examined the proteomics profiles of the female and male left and right gonads at embryonic day 6.5 (E6.5) with the label free tandem mass spectrometry proteomics technique. The relative protein abundance of the left and right gonads of female and male embryos was determined to identify their differential proteins. Overall, a total of 7726 proteins were identified, of which 79 and 54 proteins were significantly different in female and male right gonads compared with female left gonads and male left gonads respectively. Bioinformatics analysis showed that the proteins DMRT1, ZFPM2, TSHZ3 were potentially associated with the degeneration of the right gonads in female embryos. The proteomics in this study provide clues for further elucidation of the pathways of sex determination, sex differentiation, and right gonadal degeneration in birds.

show abstract

The homeobox gene TGIF1 is required for chicken ovarian cortical development and generation of the juxtacortical medulla

Cited by 12 publications

References 116 publications

DMRT1 regulation ofTOX3modulates expansion of the gonadal steroidogenic cell lineage

DMRT1 regulation ofTOX3modulates expansion of the gonadal steroidogenic cell lineage

Fadrozole-mediated sex reversal in the embryonic chicken gonad involves a PAX2 positive undifferentiated supporting cell state

Quantitative proteomics analysis of chicken embryos reveals key proteins that affect right gonadal degeneration in females

Contact Info

Product

Resources

About