Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts.

Abstract

Transforming growth factor-jB (TGF,8), when injected subcutaneously into newborn mice, induces a rapid fibrotic response, stimulates chemotaxis, and elevates the rates of biosynthesis of collagen and fibronectin by fibroblasts in vitro. We explored the molecular mechanisms of TGFft-mediated stimulation of collagen and fibronectin synthesis in cultured human foreskin fibroblasts.TGF,/ preferentially stimulated the synthesis of fibronectin and type I procollagen chains 3-5-fold as shown by polypeptide analysis. Concomitant elevation in the steady state levels of messenger RNAs (mRNAs) coding for type I procollagen and fibronectin also occurred but without a net increase in the rate of transcription of either of these genes. The preferential stabilization of mRNAs specifying type I procollagen and fibronectin provides a partial explanation for the mechanisms by which TGFft enhances the synthesis of type I procollagen and fibronectin in mesenchymal cells.

Collagen production by macrophages in tumour encapsulation and dormancy

“…It has already been demonstrated that unspecified lymphocyte products (Johnson & Ziff, 1976;Postlethwaite et al, 1984), unspecified T-cell products (Wahl & Gately, 1983), interleukin-l, which is produced by many cells including T-cells (Goldring & Krane, 1987;Postlethwaite et al, 1988), and transforming growth factor beta, produced by T-cells and macrophages (Roberts et al, 1986;Raghow et al, 1987), influence collagen production by cultured fibroblasts. The depletion of T-cells inhibited the formation of bacterial cell wall-induced hepatic granulomas in vivo (Wahl et al, 1986), and reduced the ability of immune spleen cells to form granulomas around Schistosoma eggs in vitro (Bentley et al, 1982).…”

Overexpression of TGF-β by infiltrated granulocytes correlates with the expression of collagen mRNA in pancreatic cancer

“…Both in vitro and in vivo experimental evidence has been accumulating, showing that TGF-b stimulates the production of collagens from fibroblasts. In fact, cultured fibroblasts increased the production of collagen from three-to five-fold when incubated with appropriate concentrations of TGF-b (Raghow et al, 1987;Varga et al, 1987). When TGF-b was directly injected into the subcutaneous tissue of newborn mice, accelerated fibrosis, that is, activation of fibroblasts to produce collagens, was demonstrated (Roberts et al, 1986;Shinozaki et al, 1997).…”

Transforming growth factor β receptor induction in herniated intervertebral disc tissue: an immunohistochemical study

“…TGF-β stimulates extracellular matrix component formation and regulates cellular growth and extracellular proteolysis [3,9,11,19,21]. It participates in angiogenesis and in the early phase of inflammation [13,18,21].…”