Transformation of Mungbean Plants for Salt and Drought Tolerance by introducing a gene for an osmoprotectant glycine betaine

Baloda, Anila; Madanpotra, Seema; aiwal, P.K.J

doi:10.19071/jpsp.2017.v3.3148

Cited by 36 publications

(12 citation statements)

References 30 publications

(38 reference statements)

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“…In blackgram, an efficient plant regeneration method through direct multiple shoot organogenesis from cotyledonary explants was established by Saini, Jaiwal, and Jaiwal () followed by development of transgenics for herbicide tolerance (Muruganantham, Amutha, Selvaraj, Vengadesan, & Ganapathi, ) and insect tolerance (Das, Bhagat, & Shree, ). In greengram, Baloda and Madanpotra () developed plants with salinity and drought tolerance plants by introducing a gene for an osmoprotectant glycine betaine.…”

Section: In Vitro Gene Transfermentioning

confidence: 99%

Potential, constraints and applications of in vitro methods in improving grain legumes

et al. 2018

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Grain legumes, the important constituents of sustainability-based cropping systems and energy-limited vegetarian diets have long been the subject of scientific research. Tremendous technological strides were made in the so-called orphan crops, in terms of both varietal improvement and generation of basic information. Despite recalcitrancy and high genotype dependency, in vitro culture techniques such as organogenesis, in vitro mutagenesis, embryo rescue and in vitro gene transfer have been deployed for improvement of several grain legumes and these played an important role in introgression of desirable genes from related and distant species and creation of additional genetic variability. Stable and reproducible regeneration protocols resulted in the development of genetically modified chickpea, pigeon pea, cowpea, mungbean, etc., while embryo rescue was deployed successfully for recovery of interspecific recombinants, a few of them exploited for the development of commercial cultivars. Nevertheless, doubled haploidy witnessed limited success and protoplast regeneration and in vitro mutagenesis remained of academic interest.The present review focuses on the progress, achievements, constraints and perspectives of using in vitro technology in grain legume improvement.

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Section: In Vitro Gene Transfermentioning

confidence: 99%

Potential, constraints and applications of in vitro methods in improving grain legumes

et al. 2018

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“…Proteins and carbohydrates of V. radiata are easily digestible and create less flatulence than proteins derived from other legumes. V. radiata is highly sensitive to salty, and desiccated soils, and variations of temperature (very low or very high), during the flowering and seed/pod development stages, result in heavy losses to productivity (Baloda et al 2017). V. radiata fixes atmospheric nitrogen via root rhizobial symbiosis and improves soil fertility (Yaqub et al 2010).…”

mentioning

confidence: 99%

In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiata

Vaish

Awasthi

Tiwari

et al. 2018

Genome

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Plant glutathione S-transferases are integral to normal plant metabolism, and biotic and abiotic stress tolerance. GST gene family has been characterized in diverse plant species using molecular biology and bioinformatics approaches. In the current study, in silico analysis identified 44 GSTs in Vigna radiata. Of the total 44 GSTs identified, chromosomal locations of 31 GSTs were confirmed. The pI value of GST proteins ranged from 5.10 to 9.40. The predicted molecular weights ranged from 13.12 to 50 kDa. Subcellular localization analysis revealed that all GSTs were predominantly localized in the cytoplasm. The active site amino acids were confirmed to be serine in tau, phi, theta, zeta and TCHQD; cysteine in lambda, DHAR and omega; and tyrosine in EF1G. The gene architecture conformed to the 2 exon-1 intron and 3 exon-2 intron organization in case of tau and phi classes, respectively. MEME analysis identified 10 significantly conserved motifs with the width of 8 to 50 amino acids. The motifs identified were either specific to a specific GST class, or were shared by multiple GST classes. The results of the current study will be of potential importance in the characterization of GST gene family in V. radiata, an economically important leguminous crop.

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“…Bharadwaj et al [181] cloned a stress-responsive candidate gene SKP1 from mungbean. Stable transformation and expression of transgene (codA gene) for an osmoprotectant glycine betaine have also been achieved in mungbean through Agrobacterium mediated transformation system to improve the drought tolerance response [187]. Since drought stress is a quantitatively inherited trait, engineering crop for single gene integration is practically not feasible.…”

Section: Exploring Gene Families and Transcriptional Factors As Drought-responsive Markersmentioning

confidence: 99%

Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives

Singh

Tiwari³

et al. 2021

Agronomy

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Drought stress is considered a severe threat to crop production. It adversely affects the morpho-physiological, biochemical and molecular functions of the plants, especially in short duration crops like mungbean. In the past few decades, significant progress has been made towards enhancing climate resilience in legumes through classical and next-generation breeding coupled with omics approaches. Various defence mechanisms have been reported as key players in crop adaptation to drought stress. Many researchers have identified potential donors, QTLs/genes and candidate genes associated to drought tolerance-related traits. However, cloning and exploitation of these loci/gene(s) in breeding programmes are still limited. To bridge the gap between theoretical research and practical breeding, we need to reveal the omics-assisted genetic variations associated with drought tolerance in mungbean to tackle this stress. Furthermore, the use of wild relatives in breeding programmes for drought tolerance is also limited and needs to be focused. Even after six years of decoding the whole genome sequence of mungbean, the genome-wide characterization and expression of various gene families and transcriptional factors are still lacking. Due to the complex nature of drought tolerance, it also requires integrating high throughput multi-omics approaches to increase breeding efficiency and genomic selection for rapid genetic gains to develop drought-tolerant mungbean cultivars. This review highlights the impact of drought stress on mungbean and mitigation strategies for breeding high-yielding drought-tolerant mungbean varieties through classical and modern omics technologies.

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Transformation of Mungbean Plants for Salt and Drought Tolerance by introducing a gene for an osmoprotectant glycine betaine

Cited by 36 publications

References 30 publications

Potential, constraints and applications of in vitro methods in improving grain legumes

Potential, constraints and applications of in vitro methods in improving grain legumes

In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiata

Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives

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