Transformation of microspore-derived embryos of winter oilseed rape (Brassica napus L.) by usingAgrobacterium tumefaciens

Cegielska-Taras, Teresa; Pniewski, Tomasz; Szała, Laurencja

doi:10.1007/bf03195632

Cited by 23 publications

(20 citation statements)

References 22 publications

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“…These DH lines were chosen from two populations which were obtained by using an in vitro culture of isolated microspores (Cegielska-Taras et al, 2002). In the study, a H×Z population of doubled haploids was developed from F1 hybrids obtained from the cross of DH H 2 26 (black seeds) × DH Z114 (yellow seeds) and consisted of 27 DH lines.…”

Section: Methodsmentioning

confidence: 99%

Study of variation of tocochromanol and phytosterol contents in black and yellow seeds of Brassica napus L. doubled haploid populations

Cegielska-Taras

Nogala‐Kałucka

Szała

et al. 2016

Acta Sci Pol Technol Aliment

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Background. In the study, an analysis of tocopherols, plastochomanol-8 and phytosterols was conducted using DH lines obtained from F1 hybrids of reciprocal crosses between yellow-and black-seeded lines. Material and methods. The biological material for the study consisted of two DH populations of winter oilseed rape obtained from F1 hybrids of reciprocal crosses between two DH lines: yellow-and black-seeded. Seed color was determined using a ColorFlex spectrophotometer. Fat content was determined via pulsed NMR. The levels of tocopherols, and plastochromanol-8 are analyzed using HPLC. Phytosterol contents and composition were determined by the GC method. Results. The fat content of the black-seeded parental line was 49% and this was higher than that of the yellow-seeded parental line (44%). The fat content of DH line populations ranged from 44 to 51%. Total tocopherol content ranged from 460 to 602 mg/kg and the α-T/γ-T ratio was from 0.66 to 1.09. In parental lines H 2 -26 and Z-114 the total tocopherol content was 534 and 525 mg/kg, but the α-T/γ-T ratios were 0.81 and 1.21, respectively. The yellow-seeded parental line (Z-114) was characterized by a higher PC-8 content (81 mg/kg) than the H 2 -26 black-seeded parental line (58 mg/kg). The largest part of the total phytosterol content in seeds of both populations was β-sitosterol from 976 to 2148 mg/kg, followed by campasterol, from 636 to 1364 mg/kg, and brassicasterol from 375 to 678 mg/kg. The total tocopherol content ranged from 462 to 595 mg/kg (population HxZ) and from 460 to 602 mg/kg (population ZxH). Signifi cantly positive correlations were observed between the seed color with α-T (r = 0.38, p < 0.01), γ-T (r = -0,34, p < 0.01) and PC-8 content (r = 0.29, p < 0.01). Correlations between the seed color with total tocopherol and total phytosterol content were not noted. Conclusion. Considering the range of genetic variation among doubled haploids of two populations, selected DH lines may be good parents for further breeding programs focused on increasing the amount and improving the quality of oilseed rapeseed oil. However, further studies will also be made to determine the infl uence of the environment on bioactive compounds in rapeseed oil. Cross direction of parental DH lines: yellow-and black-seeded has some infl uence, albeit not statistically signifi cant, on the diversity of doubled haploid in their populations with regard to average fat, tocochromanol and phytosterol content.

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Section: Methodsmentioning

confidence: 99%

Study of variation of tocochromanol and phytosterol contents in black and yellow seeds of Brassica napus L. doubled haploid populations

Cegielska-Taras

Nogala‐Kałucka

Szała

et al. 2016

Acta Sci Pol Technol Aliment

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“…DHs obtained by tissue cultures of microspores and MDEs show uniformity, and as a complete homozygous, they can also be efficiently used in genetic transformation (Fukuoka et al, 1998;Nehlin et al, 2000;Takahata et al, 2005;Cegielska-Taras et al, 2008). The gene introduced into a haploid genome, which in this case was an MDE and subsequent chromosome doubling, gives rise to a homozygous transgenic oilseed rape (Cegielska-Taras et al, 2008b;Cegielska-Taras and Pniewski, 2011).…”

Section: Genetic Modification Of B Napusmentioning

confidence: 99%

Doubled haploids as a material for biotechnological manipulation and as a modern tool for breeding oilseed rape ( Brassica napus L.)

Cegielska-Taras¹,

Szała²,

Matuszczak³

et al. 2015

bta

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Haploids and doubled haploids (DHs) have been produced in Brassica ssp. using anther or isolated microspore cultures. Since 1982, when Lichter developed a method of isolated microspores culture, this technique has been constantly improved and modified. Haploids and DH of Brassica napus have been extensively used in genetic studies, such as gene mapping, location of quantitative trait locus, marker / trait association studies, and genomics. Furthermore, the oilseed rape haploid induction technique can nowadays be efficiently combined with several other plant biotechnological techniques, enabling several novel breeding achievements, such as mutation breeding, the breeding of hybrid varieties, genetic transformation, and resynthesis of B. napus. The development and improvement of in vitro androgenesis of oilseed rape allowed DH populations to be obtained on a large scale. Today DH lines are used in the genetic analysis of quantitative traits and to analyze the impact of the environment on the yield and quality of the seeds. Currently, DH technology has become more efficient and hence can be widely applied in winter oilseed rape breeding. Additionally, DH technology is used to shorten the process of breeding new varieties. An open pollinated B. napus cv. Monolit (Plant Breeding Strzelce Ltd., Co.) is the first Polish winter oilseed rape variety obtained using DH technology. The breeding cycle of this variety is about four years shorter compared to a classical breeding program. The second Polish cultivar obtained using DH technology, cv. Brendy (Plant Breeding Smolice Ltd., Co.) was developed in a similarly short period. In the breeding of new oilseed rape hybrid varieties, homozygous restorer lines are also exploited. Our basic research used oilseed rape DH technology with a focus on improving the qualitative traits of oilseed rape and is presented here, based on the major researches conducted worldwide as well as on the results obtained at the Plant Breeding and Acclimati

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“…In addition, microspores, MDEs and DH plants are being used for transformation studies. Different approaches have been reported, using Agrobacterium tumefaciens (Dormann et al 2001;Cegielska-Taras et al 2008), particle gun bombardment (Fukuoka et al 1998;Chen and Beversdorf 1994;Nehlin et al 2000), or microinjection (Guerche et al 1987;Jardinaud et al 1993;Huang 1992).…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

et al. 2016

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In Brassica species microspore derived doubled haploid lines are an important tool in breeding and research. A limiting step in the production of doubled haploid lines is the diploidization of the microspores. Strong differences have been observed in diploidization rates between different genotypes but little is know about the genetic control of these differences. To identify genomic regions that carry genetic factors controlling the diploidization rate of isolated microspores of rapeseed, marker segregations were compared between segregating populations of diploid and haploid microspore derived embryos and a BC 1 from a cross between 'Express 617' and 'RS239'. After map construction ten intervarietal substitution lines from the same cross were selected with donor segments covering five genomic regions that showed a pattern of skewed marker segregations across the three populations indicative of the segregation of genes controlling the diploidization rates. The diploidization rates of microspores of the ten lines ranged from 23.9 to 58.7 % while the recurrent parent 'Express 617' showed a rate of 52.5 %. For three lines the diploidization rates were significantly lower (P = 0.05) than the rate of 'Express 617'. By comparing donor segments between the significant and the non-significant lines, seven genomic regions that cover just between 4.17 and 6.16 % of the rapeseed genome were identified that may contain genetic factors controlling diploidization rates in rapeseed. In addition, one marker was found that has a high probability to be linked to such a factor. The significant lines represent an ideal material for further in depth studies of this trait.

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Transformation of microspore-derived embryos of winter oilseed rape (Brassica napus L.) by usingAgrobacterium tumefaciens

Cited by 23 publications

References 22 publications

Study of variation of tocochromanol and phytosterol contents in black and yellow seeds of Brassica napus L. doubled haploid populations

Study of variation of tocochromanol and phytosterol contents in black and yellow seeds of Brassica napus L. doubled haploid populations

Doubled haploids as a material for biotechnological manipulation and as a modern tool for breeding oilseed rape ( Brassica napus L.)

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

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