Study of variation of tocochromanol and phytosterol contents in black and yellow seeds of Brassica napus L. doubled haploid populations

Abstract

Background. In the study, an analysis of tocopherols, plastochomanol-8 and phytosterols was conducted using DH lines obtained from F1 hybrids of reciprocal crosses between yellow-and black-seeded lines. Material and methods. The biological material for the study consisted of two DH populations of winter oilseed rape obtained from F1 hybrids of reciprocal crosses between two DH lines: yellow-and black-seeded. Seed color was determined using a ColorFlex spectrophotometer. Fat content was determined via pulsed NMR. The levels of tocopherols, and plastochromanol-8 are analyzed using HPLC. Phytosterol contents and composition were determined by the GC method. Results. The fat content of the black-seeded parental line was 49% and this was higher than that of the yellow-seeded parental line (44%). The fat content of DH line populations ranged from 44 to 51%. Total tocopherol content ranged from 460 to 602 mg/kg and the α-T/γ-T ratio was from 0.66 to 1.09. In parental lines H 2 -26 and Z-114 the total tocopherol content was 534 and 525 mg/kg, but the α-T/γ-T ratios were 0.81 and 1.21, respectively. The yellow-seeded parental line (Z-114) was characterized by a higher PC-8 content (81 mg/kg) than the H 2 -26 black-seeded parental line (58 mg/kg). The largest part of the total phytosterol content in seeds of both populations was β-sitosterol from 976 to 2148 mg/kg, followed by campasterol, from 636 to 1364 mg/kg, and brassicasterol from 375 to 678 mg/kg. The total tocopherol content ranged from 462 to 595 mg/kg (population HxZ) and from 460 to 602 mg/kg (population ZxH). Signifi cantly positive correlations were observed between the seed color with α-T (r = 0.38, p < 0.01), γ-T (r = -0,34, p < 0.01) and PC-8 content (r = 0.29, p < 0.01). Correlations between the seed color with total tocopherol and total phytosterol content were not noted. Conclusion. Considering the range of genetic variation among doubled haploids of two populations, selected DH lines may be good parents for further breeding programs focused on increasing the amount and improving the quality of oilseed rapeseed oil. However, further studies will also be made to determine the infl uence of the environment on bioactive compounds in rapeseed oil. Cross direction of parental DH lines: yellow-and black-seeded has some infl uence, albeit not statistically signifi cant, on the diversity of doubled haploid in their populations with regard to average fat, tocochromanol and phytosterol content.

Lipid Metabolism and Improvement in Oilseed Crops: Recent Advances in Multi-Omics Studies

“…Despite having different gene expression patterns, seeds and carpels showed a synergistic relationship during seed development [105]. Similar studies have since been performed in other species [101,[106][107][108][109].…”

Doubled Haploids: Contributions of Poland’s Academies in Recognizing the Mechanism of Gametophyte Cell Reprogramming and Their Utilization in Breeding of Agricultural and Vegetable Species

“…Our institute was involved in a 5-year program of the Polish Ministry of Agriculture for the enhancement of natural antioxidants and bioactive substances, such as tocochromanols, sterols, and phenolic Acta Societatis Botanicorum Poloniae / 2022 / Volume 91 / Article 9128 Publisher: Polish Botanical Society compounds, in oilseed rape. ese studies were realized with the use of different populations of DH lines specially obtained for this program (Cegielska-Taras et al, 2016;Siger et al, 2015Siger et al, , 2018.…”

Current Research Developments on the Processing and Improvement of the Nutritional Quality of Rapeseed (Brassica napus L.)

“…Likewise, the identification of vitamin E biosynthetic genes has facilitated biotechnological efforts to improve the nutritional value and antioxidant concentration of crop plants . The possibility to genetically convert the bulk of the tocopherol in plant organs from the γ‐ to α‐ form was first demonstrated using the model plant Arabidopsis thaliana .…”

“…[199] Likewise, the identification of vitamin E biosynthetic genes has facilitated biotechnological efforts to improve the nutritional value and antioxidant concentration of crop plants. [200,201] The possibility to genetically convert the bulk of the tocopherol in plant organs from the γto αform was first demonstrated using the model plant Arabidopsis thaliana. [202] In this experiment, the tocopherol composition of seeds was shifted from 97% γtocopherol to 95% α-tocopherol by a genetic transformation with a cDNA for the tocopherol/tocotrienol methyltransferase under control of a strong seed-specific promoter.…”