Many nontraditional vegetable oils have been introduced to the market PRACTICAL APPLICATIONSPhenolic compounds have been reported to be present in all vegetable oils, which is very important for the oxidative stability of the polyunsaturated fatty acids of these oils. Additionally, edible oils rich in natural antioxidants 1 Corresponding

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Lupin seed globulin proteins form complexes with flavonoids, predominantly apigenin C-glycosides. Enzymes typical for the gastrointestinal tract were used to hydrolyze lupin seed globulins. Release of native flavonoids as a result of the proteolysis reaction was observed. Different analytical methods such as size exclusion chromatography, HPLC-MS, and fluorescence spectroscopy (steady-state fluorescence, fluorescence anisotropy, fluorescence lifetimes) were used for a detailed characterization of this phenomenon. Flavonoids liberated from lupin globulin proteins as a result of pancreatin-catalyzed digestion were bound by γ-conglutin resistant to this enzyme. Two possible mechanisms of this interaction may be suggested: hydrogen bonding between oligosaccharide chains of glycoproteins and the sugar moieties of the flavonoid glycosides or electrostatic attraction between positively charged γ-conglutin and flavonoids partially ionized at pH 7.5.

Increased interest manifested by consumers in products rich in biologically active components makes it necessary to minimize their loss during drying and storage of raw materials for food production. In this study, the effect of drying conditions and further storage of rapeseed on changes in contents of tocopherols (T) and was examined. Seeds of rape cv. Californium, Elektra and Livius, dried after harvest using the near-ambient drying method in a bed of 2-m thickness and air heated to a temperature of 60, 80 and 100C, were used. Contents of T and PC-8 were determined immediately after drying and after 6 and 12 months of storage at 10 Ϯ 2C. Quality and quantity of antioxidants in seeds were determined using high-performance liquid chromatography. The near-ambient seed drying method resulted in a decrease in T content by 6-11%, while that for hot air drying was 4-8%. Seed storage reduced the level of T by a further 23-30%. Similar dependences were found for PC-8. The analyzed results demonstrated the effect of varietal differences, drying conditions and storage time on the contents of T and PC-8 in rapeseed. PRACTICAL APLICATIONSDrying of rapeseed is one of the most energy-intensive stages of its production. At the same time, one needs to consider that these seeds, as 3 Corresponding biological material, are highly sensitive to thermal treatment. Thus, search for best drying conditions with special emphasis on the preservation of bioactive components that affect human health is necessary. The analyses carried out compared the range of loss of natural antioxidants, tocochromanols, during drying and further storage of rapeseed dried using an energy-saving nearambient drying and dried using hot air. Near-Ambient DryingSeeds of cv. Californium were dried using the near-ambient method from initial moisture content of 16.2% on a farm belonging to the Agricultural University of Poznań (at present, renamed to the Poznań University of Life Sciences). The Experimental Station presented by Gawrysiak-Witulska et al. (2008) was built on the basis of a 28-ton metal silo with a diameter of 3.2 m, equipped with a computer controller (type BIT-04) of the near-ambient drying process with a set of measurement probes, a fan and an electronic air heater. During the experiment, the BIT-04 controlled the operation of the air heater so that the air blown into the silo had an adequate drying potential. The experiment 526 M. GAWRYSIAK-WITULSKA, A. SIGER and M. NOGALA-KALUCKA

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