Transformation of horse skin cells by type‐c sarcoma viruses

Self Cite

Neoplastic transformation of fetal lamb kidney (FLK) cells by bovine leukemia virus (BLV) is reported. Morphological transformation was observed in FLK cells within a few weeks of BLV inoculation. BLV-transformed FLK cells had the following properties generally associated with viral transformation: (1) altered morphology; (2) increased growth rate; (3) colony formation in soft agar medium; (4) formation of large cell aggregates and growth in this aggregate form above an agar base; and (5) tumorigenicity in nude mice. The transformed cells contained BLV-specific p24 and gp51 antigens and were virus producers. BLV was detected in transformed cells by syncytial assay. The transformed cells yielded high-titered viral reverse transcriptase activity and viral particles. The transformed cells contained marker chromosomes (M1, M2, M3, M4 and M7) and a large submetacentric chromosome, but control cells did not. Thus, we demonstrated the neoplastic transformation of FLK cells by BLV.

Section: Resultssupporting

confidence: 65%

Neoplastic transformation of fetal lamb kidney cells by bovine leukemia virus

Rhim

Kraus

Arnstein

1983

Self Cite

“…Syncytia did not form when 11 8 M G cells, the parent KC free of RSV, were co-cultivated with the BV-infected cells or when non-infected control cells were grown with the KC cells. Rat XC cells carrying the RSV genome (Svoboda, 1960) have been successfully used to develop a mixed cytopathogenicity test for type-C viruses of murine (Klement et al, 1969;Rowe et al, 1970), feline (Rangan et al, 1972a) and Simian origin (Rangan et al, 1972b). However, our attempts to develop a similar XC test for BV failed, indicating that, while BV can interact effectively with RSV genome, it will not do so in the rat cells.…”

Section: Kc Co-cultivationmentioning

confidence: 99%

Interactions between endogenous baboon type‐C virus and oncogenic viruses. I. Syncytium induction and development of infectivity assay

Ahmed¹,

Korol²,

Larson³

et al. 1975

“…Ki-MSV, originally isolated as a murine erythroblastosis virus (Kirsten et al, 1967) acquired the capacity to induce sarcomas in vivo and to transform fibroblastic cells in vitro following passage in rats (Kirsten and Mayer, 1967), and has recently been shown to have replaced about 30% of the genomic sequences of the original murine erythroblastosis virus with rat-specific sequences (Roy-Burman and Klement, 1975). Ki-MSV appears to have a wider host range than other members of the MSV group (Rhim, 1975). The observations reported here also widen the range of organs known to be susceptible to transformation by Ki-MSV to include a secretory epithelial tissue of mesodermal origin and demonstrate that carcinomas, as well as sarcomas and neoplasms of the hematopoietic system, can be induced by this oncogenic agent.…”

mentioning

confidence: 99%

Transformation of cultured rat adrenocortical cells by kirsten murine sarcoma virus (ki‐msv)

Auersperg

Goddard

Hudson

et al. 1977

Two-week-old primary cultures of normal adult rat adrenal cortex were exposed to Kirsten murine sarcoma virus (KiMSV). Within a week, the adrenal cells, which are normally fusiform and aligned in parallel, became pleomorphic and piled up extensively. Saturation density increased from 5-10 x 10(4) to 5-10 x 10(5) cells/cm2, population doubling time during exponential growth decreased from 36-40 to 16h, acid production increased and the growth rate became independent of a reduction in serum concentration from 10% to 1%. Inoculation of 2 x 10(6) of these transformed cells into immuno-depressed rats produced rapidly growing tumors within 1 week. Histologically, the tumors were pleomorphic carcinomas with areas ranging from anaplasia to near-normal, highly differentiated adrenocortical tissue. In addition to histologic evidence of differentiation, metabolic studies using 14C-prognenolone showed that the transformed cells were capable of 20alpha reduction and delta5,3beta dehydrogenation, both characteristic of normal steroid-secreting tissues. The transformed adrenocortical cells produced infectious C-type virus as indicated by electron microscopy, 3H-uridine incorporation, and focus formation in NRK (normal rat kidney) cultures. The neutralization pattern of this virus resembled that of authentic Ki-MSV. The transformation of adrenocortical cells by K-MSV demonstrates the capacity of this agent to induce carcinomas in differentiated cells after short-term culture, and widens the range of tissues known to be susceptible to K-MSV to include a secretory epithelium of mesodermal origin.