Neoplastic transformation of fetal lamb kidney cells by bovine leukemia virus

Rhim, Johng S.; Kraus, Matthias H.; Arnstein, Paul

doi:10.1002/ijc.2910310620

Cited by 9 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BLV shares several biological properties with HTLV-I (Burny et al, 1980;Weiss, 1982) and, in fact, appears to be evolutionarily related judging from the appreciable homology between their core proteins (Oroszlan et al, 1982;Copeland et al, 1983b) and from the unique structures of their LTRs (Sagata et al, 1984a). The ability of BLV to transform cells in vitro (Onuma et al, 1981;Rhim et al, 1983), the absence of preferred chromosomal sites for proviral integration (Kettmann et al, 1983; our unpublished observation) and the presence of 5' half-truncated proviruses in the leukemic cells (Kettmann et al, 1982) also suggest that the 3' half of the BLV genome possesses some gene implicated in cellular transformation. Collectively, it appears that the genomic structure of BLV has substantial similarity to that of HTLV-I, although this has not been elucidated so far at a molecular (nucleotide) level.…”

Section: Introductionmentioning

confidence: 80%

Comparison of the entire genomes of bovine leukemia virus and human T-cell leukemia virus and characterization of their unidentified open reading frames.

Sagata¹,

Yasunaga²,

Ohishi³

et al. 1984

The EMBO Journal

View full text Add to dashboard Cite

We have compared the sequence of the entire genomes of bovine leukemia virus (BLV) and human T‐cell leukemia virus type I (HTLV‐I). Both the gag and pol genes show overall strong homologies indicating the close evolutionary relationship of the two retroviruses. However, a surface glycoprotein portion of the env gene shows no appreciable homology, which probably reflects a difference in their host ranges. The 3′ end portion of the BLV genome (designated as pXBL) contains an unidentified long open reading frame that has a typical protein‐coding property. The potential product of this open reading frame may be a glycoprotein of approximately 40 000 daltons. We note that its amino acid sequence shows low but appreciable homology, especially in its N‐terminal quarter, to that of the HTLV‐I counterpart (pX product), and we thus suggest that BLV pXBL and HTLV‐I pX have diverged from a common ancestral gene. It is tentatively concluded that both the putative pXBL and pX products are respectively produced from a spliced mRNA.

show abstract

Section: Introductionmentioning

confidence: 80%

Comparison of the entire genomes of bovine leukemia virus and human T-cell leukemia virus and characterization of their unidentified open reading frames.

Sagata¹,

Yasunaga²,

Ohishi³

et al. 1984

The EMBO Journal

View full text Add to dashboard Cite

show abstract

“…The presence of proviral DNA was determined in blood samples by nested PCR. In nested PCR, DNA from FLK-BLV cell line was used as positive control (20).…”

Section: Methodsmentioning

confidence: 99%

Application of in situ PCR for the Detection of Bovine Leukaemia Virus (BLV) Infection in Dendritic Cell cultures

Iwan

Szczotka

Kuźmak

2014

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

The aim of the study was to develop an in situ PCR (IS-PCR) method for detection of bovine leukemia virus (BLV) in cell cultures. Samples from five BLV positive and five BLV negative cows were collected and dendritic cells (DCs) from blood, bone marrow, spleen, and lymph node were cultured. Cultures prepared from healthy animals were infected with BLV. After two weeks, the cells were tested by nested PCR and IS-PCR for the presence of proviral DNA. As a positive control adherent cell line permanently infected with BLV was used. BLV was successfully detected by IS-PCR in DCs from naturally infected cattle and DCs infected in vitro. In control, non-infected DCs, the results of the reaction were negative. The results of provirus detection by IS-PCR were similar with these performed with nested PCR. Additionally, IS-PCR provides many advantages, like specific localisation of infection and smaller number of cells needed as template for PCR.

show abstract

“…4,21,26 An amplified, outer pol product was cloned into a pGEM cloning system f and used as a positive control. Proviral DNA was also extracted from a BLV persistently infected fetal lamb kidney cell line 25 and used as an additional positive control.…”

mentioning

confidence: 99%

Detection of Bovine Leukemia Virus in Blood and Milk by Nested and Real-Time Polymerase Chain Reactions

et al. 2003

View full text Add to dashboard Cite

Concerns about retroviruses in livestock and products derived from them have necessitated the development of tests to detect the bovine leukemia virus (BLV) in blood and milk from cattle. Dairy cattle (n = 101) from 5 different geographical areas were used for this study. A nested polymerase chain reaction (PCR) identified 98% of BLV seropositive cattle (n = 80) from blood and 65% from milk, whereas real-time PCR detected 94% of BLV seropositive cattle from blood and 59% from milk. Bovine leukemia virus was also detected by PCR in approximately 10% of seronegative cattle (n = 21), most likely because of early detection before seroconversion.

show abstract

Neoplastic transformation of fetal lamb kidney cells by bovine leukemia virus

Cited by 9 publications

References 15 publications

Comparison of the entire genomes of bovine leukemia virus and human T-cell leukemia virus and characterization of their unidentified open reading frames.

Comparison of the entire genomes of bovine leukemia virus and human T-cell leukemia virus and characterization of their unidentified open reading frames.

Application of in situ PCR for the Detection of Bovine Leukaemia Virus (BLV) Infection in Dendritic Cell cultures

Detection of Bovine Leukemia Virus in Blood and Milk by Nested and Real-Time Polymerase Chain Reactions

Contact Info

Product

Resources

About