Transfer of RNA from nucleus to cytoplasm in early development of fish

Neyfakh, Alexander A.; Kostomarova, A A; Burakova, T. A.

doi:10.1016/0014-4827(72)90584-8

Cited by 7 publications

(4 citation statements)

References 3 publications

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“…Jusqu'a ce stade, le dCveloppement de l'ceuf est assurC par les prot2ines et les ARN stock& pendant I'ovogCnkse . Les nCosynthkses ne dCmarrent qu'au stade de la miblastula (Rott et Sheveleva 1968;Kafiani et al 1969;Neyfakh et al 1972;Chaudhuri et al 1979): donc, si les chocs thermiques agissent en dCtCriorant les rCserves d'origine ovocytaire, leur remplacement ne pourra &re assurC avant le stade blastula. En particulier, on peut invoquer le r61e des polyamines dont le renouvellement rapide est un facteur de la regulation du mktabolisme face aux variations de la tempkrature (A. N. Neyfakh, communication personnelle) .…”

Section: Effets Dzye're's Du Choc Thermiqueunclassified

Résistance de l'œuf de carpe commune (Cyprinus carpio L. Cyprinidae) à des chocs thermiques chauds ou froids

Jaoul¹,

Roubaud²

1982

Can. J. Zool.

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R e p le 24 Dkcembre 198 1 JAOUL, A., et P. ROUBAUD. 1982. Rksistance de l'ceuf de carpe commune (Cyprinus carpio L. Cyprinidae) B des chocs thermiques chauds ou froids. Can. J. Zool. 60: 3409-34 19. La rksistance de l'embryon de la carpe commune (Cyprinus carpio L.) B un choc thermique froid ou chaud est CtudiCe en mesurant, jusqu'h l'kclosion, les pourcentages de survie au cours d'une incubation B la tempkrature constante de 20°C. Lorsque l'incubation a lieu dans un milieu saturC en oxygkne, un choc thermique de durCe de 1 h survenant pendant la pkriode de segmentation synchrone reste sans effet dans la g a m e de tempkratures de choc 8-28°C. Au stade de la gastrula, cet intervalle s'est Ctendu jusqu'h 0,3"C du cBtC froid et il a atteint 36°C B la fin de l'kpibolie. Lorsque l'incubation a lieu dans des conditions hypoxiques, un choc postkpibolique de 1 h n'a aucun effet s'il reste dans la gamme de tempdratures 1-36°C. Au contraire, un choc froid prCblastulCen de tempkrature infkrieure ou Cgale B 12°C ou un choc chaud prkgastrulken de tempkrature supkrieure ou Cgale B 30°C suffisent en 1 h pour provoquer une baisse significative de la rksistance ultCrieure de l'embryon B l'hypoxie. Une suroxygCnation pendant la durCe du choc ne modifie pas la rksistance embryonnaire. Pendant la segmentation synchrone, la sensibilitk au choc thermique froid peut fluctuer (peut-&re en relation avec le cycle mitotique); la thermosensibilitk dkpend aussi des qualitCs physiologiques des gamttes. Les caractkristiques de la rksistence B un choc thermique sont comparkes avec les profils des tempCratures dCcrites comme favorables pour des incubations en laboratoire B tempkrature constante ou pour les dCveloppements dans les fraytres naturelles. L'hypoxie ou l'hyperoxie ne peuvent &re le facteur dkterminant dans I'action des chocs thermiques; diverses hypoth2ses sont passkes en revue pour expliquer l'acquisition par I'embryon d'une rksistance croissante aux variations de la tempkrature du milieu. JAOUL, A., and P. ROUBAUD. 1982. Rksistance de l'ceuf de carpe commune (Cyprinus carpio L. Cyprinidae) B des chocs thermiques chauds ou froids. Can. J. Zool. 60: 3409-3419. The resistance of common carp (Cyprinus carpio L.) eggs to hot and cold shock has been studied by measuring the survival rate during the course of an incubation at a constant temperature of 20°C. When incubation was pursued in oxygen-saturated conditions, a 1-h-long thermal shock which occurred during the synchronous segmentation stage had no effect within the temperature range of 8-28°C. At gastrula stage, this interval reached 0.3"C and at the end of epiboly it reached 36°C. With hypoxic incubation conditions, a 1-h postepibolic shock had no effect within the range of 1-36°C. On the contrary, a 12°C (or less) preblastulean cold shock or a 30°C (or more) pregastrulean hot shock induced a significant loss of further resistance to hypoxy. An overoxygenation during the shock did not increase the embryonic resistance. During the synchronous segmentation, the sensitivity to cold s...

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Section: Effets Dzye're's Du Choc Thermiqueunclassified

Résistance de l'œuf de carpe commune (Cyprinus carpio L. Cyprinidae) à des chocs thermiques chauds ou froids

Jaoul¹,

Roubaud²

1982

Can. J. Zool.

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“…Grain counts were recalculated per cytoplasm and nuclei volumes. The data on cell and nuclei volumes were taken from [13,14]. A comparison of the results from tables 1 and 2 shows that in both cases the nuclei comprise 39-40% of the labelled RNA.…”

Section: Resultsmentioning

confidence: 99%

“…Blastoderms isolated from the yolk as in [ 11 ] were incubated in the presence of [3H]uridine [12]. All autoradiographic procedures were as in [13,14].…”

Section: Methodsmentioning

confidence: 99%

On the existence of free informosomes in the cytoplasm

et al. 1978

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“…The labelling intensity was estimated by counting the number of silver grains over background on equatorial sections of nuclei. The intensity of labelling in the whole nuclear volume is given by the formula valid for tritium: N = (2Dl3Qn, where N = total number of grains, D = diameter of the nucleus, I= section thickness (1 pm) and n = number of grains on the equatorial section (Neyfakh et al, 1972). The assimilation of the nuclei into spheres introduces a systematic error which is, however, the same for all early stages and thus the latter remain comparable.…”

Section: Introductionmentioning

confidence: 99%

Onset of RNA synthesis and poly (A) content of early rabbit embryos. Comparison with sheep

Kaňka¹,

Je²,

Šutovský³

1993

Reprod. Nutr. Dev.

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Summary ― RNA synthesis in 2-32 cell embryos, as assessed by a-amanitin-sensitive 3 H-uridine incorporation, was first detectable in 4-cell stage rabbit and 8-cell stage sheep embryos. In the rabbit, uridine incorporation was detectable at the 2-cell stage but was unaltered by a-amanitin, indicating synthesis of non-polymerase II-dependent RNA species. Initiation of mRNA synthesis as determined by in situ hybridization with 3 H-poly (U) probe was first detectable in late 2-cell stage rabbit and 4-cell stage sheep embryos. In the rabbit embryos, nuclear labelling increased from the late 2-cell stage to the 16-cell stage, following a pattern similar to that of 3 H-uridine incorporation. In contrast, the intensity of cytoplasmic labelling decreased from the 1-to the 8-cell stage and then increased up to the 32-cell stage. In sheep embryos, nuclear labelling by the poly (U) probe increased from the 4-to the 16-cell stage. It is concluded that initiation of transcription of the embryonic genome (mRNA) can be detected via the current methods used at the 4-cell stage in the rabbit and the 8-cell stage in the sheep.

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Transfer of RNA from nucleus to cytoplasm in early development of fish

Cited by 7 publications

References 3 publications

Résistance de l'œuf de carpe commune (Cyprinus carpio L. Cyprinidae) à des chocs thermiques chauds ou froids

Résistance de l'œuf de carpe commune (Cyprinus carpio L. Cyprinidae) à des chocs thermiques chauds ou froids

On the existence of free informosomes in the cytoplasm

Onset of RNA synthesis and poly (A) content of early rabbit embryos. Comparison with sheep

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