Transfer of cells with uptaken nanocomposite, magnetite-nanoparticle functionalized capsules with electromagnetic tweezers

Abstract

Targeted cell delivery via magnetically sensitive microcapsules of an applied magnetic field would advance localized cell transplantation therapy, by which healthy cells can be introduced into tissues to repair damaged or diseased organs. In the present research, we implement magnetically sensitive cells via an uptake of microcapsules containing magnetic nanoparticles in their walls. As is shown in an example of the MA-104 cell line, the magnetic polyelectrolyte multilayer capsules have no toxicity effect on the cells after internalization. Microscopy methods have been used to evaluate the uptake of capsules by the cells. Magnetically sensitive cells are retained in the capillary flow when the magnetic gradient field is applied (<200 T m-1), but they proliferate at the site of retention for several days after the magnet is removed. As an example of cell manipulation, we have demonstrated a novel methodology for cell sheet isolation and transfer using cells impregnated with magnetic microcapsules. A weak enzyme treatment is used to facilitate tissue engineering assemblies by cell monolayer deposition. This type of cell monolayer assembly has provided a 3D tissue engineering construction using an externally applied magnetic field, which is modelled in this study. The approach presented in this work opens perspectives for preclinical studies of tissue and organ repair.

Hierarchy of Hybrid Materials—The Place of Inorganics-in-Organics in it, Their Composition and Applications

“…Introduction of inorganic particles into hydrogel coatings allows the production of catalytically active interfaces (Agrawal et al, 2013), while on the other hand optical properties of hydrogels can be controlled through the addition of nanoparticles (Agrawal et al, 2013). Incorporation of magnetic nanoparticles into organic coatings has been used for the induction of release functionality (Hu et al, 2008) and manipulation of tissue for tissue engineering (Vidiasheva et al, 2018). Magnetic nanoparticles have also been used for adding magneto-responsive properties to magnetic hydrogels (Jaiswal et al, 2014).…”

Polymer Capsules with Hydrophobic Liquid Cores as Functional Nanocarriers

“…Magnetically responsive, liquid core capsules could be an attractive option for both drug delivery systems and cell therapy, including stem cells and immune cells used for regenerative medicine, and the treatment of genetic and cancer defects. Such systems allow for the magnetic guidance of living cells via uptake of microcapsules containing magnetic nanoparticles combined with the possibility of delivering bioactive molecules, either through sustained release or triggered release [ 173 , 174 , 175 ]. Podgórna et al introduced the method in which the hydrophobic suspension of Fe 3 O 4 nanoparticles was coated with a cationic polyelectrolyte layer with the aid of oil-soluble surfactant-docusate sodium salt [ 176 ].…”

Magnetically Actuated Manipulation and Its Applications for Cartilage Defects: Characteristics and Advanced Therapeutic Strategies

“…Even removing the external MF, m-MSCs could still remain at the defect site (Hori et al, 2011). After the MF exposure, m-MSCs have a good adhesion, like cell sheets covering to the defect surface, which can favor cell communication and signaling among cells that are capable of spontaneous matrix formation and further promote chondrogenesis and cartilage regeneration (Goncalves et al, 2017;Vidiasheva et al, 2018). Thus, MTD could make a potential homogeneous distribution of m-MSCs to cover the defect area with high adhesion rate, which have trended toward promoted chondrogenic differentiation and cartilage regeneration.…”