Transepithelial Transport of Macromolecular Substances in IL-4 Treated Human Intestinal T84 Cell Monolayers

Abstract

The effect of interleukin-4 (IL-4), a cytokine associated with allergy and inflammation, on the permeability of the intestinal epithelium was investigated. IL-4 reduced transepithelial electrical resistance (TER) and increased permeation to horseradish peroxidase (HRP) and Lucifer Yellow (LY) of human intestinal T84 cell monolayers. The increased permeation due to IL-4 treatment was also observed at 4 C. The permeability of T84 cell monolayers to -lactogulobulin (-Lg), ovalbumin (OVA), and fluorescein isothiocyanate (FITC)-dextran of various molecular sizes was also high in the IL-4-treated cell monolayers. Sodium azide (NaN 3 ), which inhibits ATP synthesis of the cells, did not inhibit the increases in these substances. Even 150 kDa FITC-dextran significantly permeated the T84 cells when the monolayers were treated with IL-4. These results suggest that fairly large molecules are able to permeate intestinal epithelial monolayers via the energy-independent paracellular pathway when the monolayers are exposed to excessive IL-4.

Novel and established intestinal cell line models – An indispensable tool in food science and nutrition

“…Immunoglobulin E (IgE), along with CD23, an antibody involved in allergic reactions may facilitate transepithelial antigen sampling (Li, et al, 2006). Similarly, allergenic proteins such as b-lactoglobulin and ovalbumin were able to permeate the intestinal monolayer via energy-independent paracellular pathways in the presence of excessive interleukin-4 (IL-4) concentrations (Mochizuki, Satsu, Totsuka, & Shimizu, 2009).…”

TGFβs Modulate Permeability of the Blood-Epididymis Barrier in an In Vitro Model

“…Based on our preparatory experiments [29] we established a BEB in vitro model. In this model a highly efficient barrier of 840 Ω x cm 2 was produced, which is in the range of TER observed in gut epithelium in vitro models [33] and is higher than described for in vitro models of the blood-testis barrier with less than 250 Ω x cm 2 [34] , [35] . In in vitro models of renal proximal tube up to 240 Ω x cm 2 were observed [36] .…”

Epithelial models to study food allergen-induced barrier disruption and immune activation

“…Similar aspects may apply for certain food derived allergens such as Peach LTP and peanut allergens (Table 2). In addition, IL-4 and IL-13 produced by T H 2 cells and/or ILC2, and tryptase secreted by mast cells, can enhance epithelial permeability via the IL-4/IL-13 receptor or PAR2 receptor respectively [19][20][21][22]. Beyond allergens increasing paracellular permeability and crossing the epithelial barrier via the transcellular route, IgE-allergen complexes can be transported over IEC via the low affinity IgE receptor CD23b [23].…”