Novel and established intestinal cell line models – An indispensable tool in food science and nutrition

Langerholc, Tomaž; Maragkoudakis, Petros A.; Jan, Wollgast; Gradišnik, Lidija; Cencič, Avrelija

doi:10.1016/j.tifs.2011.03.010

Cited by 115 publications

(93 citation statements)

References 93 publications

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“…As in other cancer-derived cell line models, signifi cant differences in the gene expression of transporters and metabolic enzymes from the normal human intestinal cells can affect the suitability of the model in refl ecting the in vivo permeability (Langerholc et al 2011 ). Similar protein expression was found in HT29 cells and the corresponding intestinal scrapings, of which some appear to be characteristic to human intestinal epithelium in vivo (Lenaerts et al 2007 ).…”

Section: Relevance To Human In Vivo Situationmentioning

confidence: 93%

HT29 Cell Line

Martínez-Maqueda

Miralles

Recio

2015

The Impact of Food Bioactives on Health

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The human colon adenocarcinoma cell line HT29, is not only used to study the biology of human colon cancers, but it is receiving special interest in studies focused on food digestion and bioavailability due to the ability to express characteristics of mature intestinal cells. In the differentiated phenotype, they are able to form a monolayer with tight junctions between cells and a typical apical brush border. In addition, these differentiated cells express brush-border-associated hydrolases typical of the small intestine although the enzymatic activity is lower than that found in vivo. Although they represent a valuable model due to their similarities with enterocytes of the small intestine, their limitations and the relevance to the in vivo situation are also considered in this chapter. The application of this cell line to transport studies of drugs and food compounds is illustrated, especially when the effect of the mucus layer is considered or used as co-culture in combination with Caco-2 cells. They have also been frequently used to study the intestinal immune response to bacterial infection, and microorganism survival, adhesion or invasion. Finally, the use of these cells to evaluate the effect of several food compounds and mucin secretion is summarized. Keywords HT29 • Cell differentiation • Transport studies • Intestinal cell OriginThe human colon adenocarcinoma cell line HT29 was isolated from a primary tumor of a 44 years old Caucasian female in 1964 by Fogh and Trempe ( 1975 ). Since then, many cell lines have been derived from human colon cancers. Initially, this cell line was used to study different aspects of the biology of human cancers. However, these cells have attracted attention due to the fact they were able to express characteristics of mature intestinal cells, such as enterocytes or mucus producing cells.

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Section: Relevance To Human In Vivo Situationmentioning

confidence: 93%

HT29 Cell Line

Martínez-Maqueda

Miralles

Recio

2015

The Impact of Food Bioactives on Health

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“…They can also participate in pathogen elimination due to their anti-invasive, antitoxin properties and anti-adhesive behaviour towards microorganisms. In addition, they can restore gut mucosal barrier integrity and function tipped by harmful stimuli, enteropathogenic or other bacterial invasion [4][5][6]. In few conclusive reviews it is discussed in detail how the probiotic bacteria can facilitate several intestinal cell protective mechanisms describing them as pivotal modulators of membrane dynamics, transepithelial permeability, epithelial responses to inflammatory mediators, ion secretion and barrier integrity through intracellular pathways.…”

Section: Introductionmentioning

confidence: 99%

Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

et al. 2012

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“…The cell culture models were proved to be a good alternative for animal studies and have emerged as one of the standard in vitro tools to predict in vivo intestinal absorption of various substances. Subsequently, they were found to be suitable to study intestinal transport of phytochemicals [Barrington et al, 2009;Deprez et al, 2001], and nowadays, cell line models are called an indispensable tool in food science and nutrition [Langerholc et al, 2011]. The studies of epithelial transport/bioavailability are performed in conditions mimicking physiological condi-tions, involving pH 6.0-7.4.…”

Section: Introductionmentioning

confidence: 99%

Stability of Phenolic Compounds Isolated from Cocoa, Green Tea and Strawberries in Hank’s Balanced Salt Solution under Cell Culture Conditions.

Kosińska¹,

Xie²,

Diering³

et al. 2012

Pol. J. Food Nutr. Sci.

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The study analysed the stability of polyphenols present in extracts isolated from cocoa, green tea and strawberries in Hank's Balanced Salt Solution buffers. The extracts were incubated under conditions commonly used for intestinal absorption experiments with human epithelial cells. The polyphenols were analysed with HPLC-DAD-FLD-MS method. The concentrations of most of the polyphenols monitored changed during 2 h of incubation. The fl avan-3-ols of cocoa exhibited similar stability and all were more stable at pH 6.5 than 7.4. The concentration of procyanidin B2 at pH 7.4 decreased to the highest extent. Green tea predominant catechins: epigallocatechin gallate and epigallocatechin, were very vulnerable in HBSS both at pH 6.5 and 7.4; their specific molecular structure may be responsible for this phenomenon. The anthocyanins of a strawberry extract were more stable at pH 6.5. The considerable increase in ellagic acid concentration at pH 7.4 might be explained by possible degradation of other compounds of the extract. It is highly recommended to perform stability tests before transport experiments in a cell culture model in order to avoid misinterpretation of results.

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Novel and established intestinal cell line models – An indispensable tool in food science and nutrition

Cited by 115 publications

References 93 publications

HT29 Cell Line

HT29 Cell Line

Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

Stability of Phenolic Compounds Isolated from Cocoa, Green Tea and Strawberries in Hank’s Balanced Salt Solution under Cell Culture Conditions.

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