Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

Gut epithelial inducible heat-shock proteins and their modulation by diet and the microbiota

“…This has been consistently demonstrated in various settings and GEC lines. 27,31,[34][35][36][37][38][39][40][41][42][43][44] However, this does not always translate to comparable results in vivo, 45 possibly because of insufficient (though significant) increases in iHSP levels.…”

“…70 Many gram-positive bacteria present in the human gut microbiota, such as Bifidobacterium breve, Lactobacillus paracasei, and Lactobacillus plantarum (but not Enterococcus faecalis), could induce expression of iHSP27 in Caco2 brush-border-expressing (Caco2-bbe) cells (Table 3). 30,39,40,43,44,71,72 A synthetic pentapeptide, also known as competent and sporulating factor from Bacillus subtilis, was shown to induce expression of HSP27 and protect gut epithelial cell (GEC) lines from oxidant injury. 42,72,73 Among gram-negative bacteria, E. coli can induce expression of gut epithelial iHSP, but some other bacteria, such as Enterobacter aerogenes, E. faecalis, and Proteus mirabilis, cannot.…”

Modulation of immunity and inflammatory gene expression in the gut, in inflammatory diseases of the gut and in the liver by probiotics

“…Paszti-Gere et al [30] (2012) investigated the immunomodulatory effect of the culture supernatant of five bacterial strains in a non-transformed cell line that was derived from porcine jejunal epithelial IPEC-J2 cells that had been previously subjected to oxidative stress with hydrogen peroxide. L. plantarum 2142 had significantly decreased the IL-8 and TNF-α mRNA levels with the concomitant upregulation of HSP70 gene expression.…”

“…cremoris FC IL-8 O'Flaherty et al [28] L. acidophilus NF-κB signaling Oksaharju et al [29] Bifidobacteria, lactobacilli, and P. freudenreichii FCER1A, FCER1G, IL-8, TNF-α, and IL-10 Paszti-Gere et al [30] L. plantarum 2142 and bifidobacteria IL-8 and TNF-α Zanello et al [31] Saccharomyces cerevisiae CNCM I-3856 PPAR-γ Latvala et al [32] Lactobacillus and Streptococcus species SOCS3 Ukena et al [33] E. coli Nissle 1917 MCP-1, MIP-2alpha and MIP-2beta Wang et al [34] L. casei Zhang TLR2, TLR3, TLR4, and TLR9 Kim et al [35] L. plantarum genomic DNA TLR2, TLR4, and TLR9 Cammarota et al [36] L. plantarum DSMZ 12028 TLR2 and TLR4 Tomosada et al [37] Eleven different probiotic strains MAPK and NF-κB pathways Gao et al [38] C. butyricum IL-8, IL-6, and TNF-α Isono et al [39] C. butyricum TO-A TLR-4 Huang et al [40] Bacillus species TLR-2 and TLR-4 Dendritic cells Bermudez-Brito et al [7] L. paracasei CNCM I-4034 TLR9, CASP8, and TOLLIP Weiss et al [48] L. acidophilus Genes encoding IFN, TLR-3, and IL-12 Rajput et al [49] S. boulardii and B. subtillis B10 MyD88, NF-κB, TLR-1, 2, 4, and 15 Latvala et al [50] Bifidobacteria, lactobacilli, and S. thermophilus THS TNF-α, IL-1β, IL-6, IL-10, IL-12, and IFN-γ Evrard et al [52] L. rhamnosus 35 IL12, TNF-α, IL1B, IL6, TGFB1, IL-23, and IL-8 Bermudez-Brito et al [55] B. breve CNCM I-4035 TLR-9 and TOLLIP Ayehunie et al [56] L. delbrueckii subsp. bulgaricus TLR-4, p38, and Iκ B Animal studies Matsumoto et al [57] B. animalis subsp.…”

Facultative to strict anaerobes ratio in the preterm infant microbiota

“…31,32 Interestingly, probiotic microorganism could also play a role as antioxidants, since strains displaying this property have been reported 33 and probiotic metabolites able to prevent the production of proinflammatory cytokines induced by oxidative stress have been recently identified. 34 Therefore, in the context of nutritional interventions the inclusion on infant formula of antioxidants, able to reduce the redox potential also in the while none of the preterm infants was exclusively in breast-feeding. In our cohort at 2 d of age only 2 preterm infants were exclusively breast-fed, 3 were on mixed feeding and 16 were on formula, while at 10 d only 3 infants remained exclusively formula-fed.…”