HT29 Cell Line

Abstract

The human colon adenocarcinoma cell line HT29, is not only used to study the biology of human colon cancers, but it is receiving special interest in studies focused on food digestion and bioavailability due to the ability to express characteristics of mature intestinal cells. In the differentiated phenotype, they are able to form a monolayer with tight junctions between cells and a typical apical brush border. In addition, these differentiated cells express brush-border-associated hydrolases typical of the small intestine although the enzymatic activity is lower than that found in vivo. Although they represent a valuable model due to their similarities with enterocytes of the small intestine, their limitations and the relevance to the in vivo situation are also considered in this chapter. The application of this cell line to transport studies of drugs and food compounds is illustrated, especially when the effect of the mucus layer is considered or used as co-culture in combination with Caco-2 cells. They have also been frequently used to study the intestinal immune response to bacterial infection, and microorganism survival, adhesion or invasion. Finally, the use of these cells to evaluate the effect of several food compounds and mucin secretion is summarized. Keywords HT29 • Cell differentiation • Transport studies • Intestinal cell OriginThe human colon adenocarcinoma cell line HT29 was isolated from a primary tumor of a 44 years old Caucasian female in 1964 by Fogh and Trempe ( 1975 ). Since then, many cell lines have been derived from human colon cancers. Initially, this cell line was used to study different aspects of the biology of human cancers. However, these cells have attracted attention due to the fact they were able to express characteristics of mature intestinal cells, such as enterocytes or mucus producing cells.

Effects of pharmacological calcimimetics on colorectal cancer cells over-expressing the human calcium-sensing receptor

“…In contrast, in Caco2 CaSR-GFP cells, the overexpression of the CaSR had no clear effect on the expression of these factors, but the treatment with NPS R-568 inhibited the transcription of the intestinal differentiation markers CDX2, DLL1 and DLL4. Both HT29 and Caco2 cell lines derive from colorectal adenocarcinomas, however they are very different in terms of genetic background and differentiation level [71,72]. Our data suggest that the cells genetic background and their pre-existing differentiation state affected the nature of the CaSR response.…”

Extending the Shelf-Life of Meat and Dairy Products via PET-Modified Packaging Activated With the Antimicrobial Peptide MTP1

“…In addition to antibacterial activity, cytotoxicity is another important parameter influencing the application of any material for industrial and medical purposes. Therefore, in order to ascertain the non-toxic behavior of MTP1-modified PETs, cell viability assay was carried out on the human colon cancer cell line HT-29, that is receiving special interest in studies focused on the effects of food products on human health (Martínez-Maqueda et al, 2015). To this aim, cell viability was determined by exposing these cells to functionalized polymers for different time intervals, using the non-conjugated PET disks as control.…”

Extractable Bacterial Surface Proteins in Probiotic–Host Interaction

“…This led to the development of recognized in vitro techniques ( Blum et al, 1999 ; Vesterlund et al, 2005 ). This includes the use of IECs lines such as Caco-2 ( Hirakata et al, 1998 ) and HT-29 ( Maoret et al, 1989 ; Gagnon et al, 2013 ; Martínez-Maqueda et al, 2015 ). Extracellular components are also used for adhesion, including laminin, fibronectin, collagen, and proteoglycan.…”