Epithelial models to study food allergen-induced barrier disruption and immune activation

Gavrović-Jankulović, Marija; Willemsen, Linette E. M.

doi:10.1016/j.ddmod.2016.09.002

Cited by 12 publications

(14 citation statements)

References 76 publications

(92 reference statements)

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“…Besides its hypothetical effects on barrier permeability, Per a 10 induces IL-6, IL-8/CXCL-8, and GM-CSF in A549 cells ( Kale and Arora, 2015 ). Finally, several food allergens with proteolytic activity affect intestinal epithelial barrier integrity, as reviewed in Gavrovic-Jankulovic and Willemsen (2015) , but the description of the underlying mechanisms is beyond the scope of this review.…”

Section: Mechanisms Of Epithelial Barrier Dysfunctionmentioning

confidence: 99%

Epithelial Barrier Dysfunction in Chronic Respiratory Diseases

2021

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Mucosal surfaces are lined by epithelial cells, which provide a complex and adaptive module that ensures first-line defense against external toxics, irritants, antigens, and pathogens. The underlying mechanisms of host protection encompass multiple physical, chemical, and immune pathways. In the lung, inhaled agents continually challenge the airway epithelial barrier, which is altered in chronic diseases such as chronic obstructive pulmonary disease, asthma, cystic fibrosis, or pulmonary fibrosis. In this review, we describe the epithelial barrier abnormalities that are observed in such disorders and summarize current knowledge on the mechanisms driving impaired barrier function, which could represent targets of future therapeutic approaches.

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Section: Mechanisms Of Epithelial Barrier Dysfunctionmentioning

confidence: 99%

Epithelial Barrier Dysfunction in Chronic Respiratory Diseases

2021

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“…63 Degradation of epithelial barrier by proteaseactive allergens facilitates the presentation of allergens to the immune system, increases allergic sensitivity, and contributes to allergic inflammatory reaction, thereby facilitating sensitivity to secondary allergens. 59,60,64 As a contact allergen, p-Phenylenediamine is used in hair dyes and causes allergic contact dermatitis. Occupationally exposed phenylenediamine induces extensive transcriptomic changes in skin barrier, including downregulation of TJ and stratum corneum proteins, even in the absence of clinical symptoms.…”

Section: Environmental Factors and The Epithelial Barrier Epithelial mentioning

confidence: 99%

Environmental factors in epithelial barrier dysfunction

Sözener

Cevhertas

Nadeau

et al. 2020

Journal of Allergy and Clinical Immunology

216

133

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The main interfaces controlling and attempting to homeostatically balance communications between the host and the environment are the epithelial barriers of the skin, gastrointestinal system, and airways. The epithelial barrier constitutes the first line of physical, chemical, and immunologic defenses and provides a protective wall against environmental factors. Following the industrial revolution in the 19th century, urbanization and socioeconomic development have led to an increase in energy consumption, and waste discharge, leading to increased exposure to air pollution and chemical hazards. Particularly after the 1960s, biological and chemical insults from the surrounding environment-the exposome-have been disrupting the physical integrity of the barrier by degrading the intercellular barrier proteins at tight and adherens junctions, triggering epithelial alarmin cytokine responses such as IL-25, IL-33, and thymic stromal lymphopoietin, and increasing the epithelial barrier permeability. A typical type 2 immune response develops in affected organs in asthma, rhinitis, chronic rhinosinusitis, eosinophilic esophagitis, food allergy, and atopic dermatitis. The aim of this article was to discuss the effects of environmental factors such as protease enzymes of allergens, detergents, tobacco, ozone, particulate matter, diesel exhaust, nanoparticles, and microplastic on the integrity of the epithelial barriers in the context of epithelial barrier hypothesis.

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“…Temperature is measured before and 30 minutes to 1 hour after allergen challenge, but this parameter can also be monitored over time. 21,22 Animals sensitized to a given food matrix or protein may display a significant reduction in body temperature (0.5-10°C) 3,4 compared with that of naive animals. For an adequate level of sensitivity, 5-16 animals per group should have their temperatures measured using a rectally inserted thermal probe, 29 but it is also possible to measure changes over time for individual animals using an electronic ID transponder implanted subcutaneously.…”

Section: Me a Surement Of Body Temper Aturementioning

confidence: 99%

“…A variety of in vitro and in vivo models have been developed that address the factors and mechanisms involved in the sensitization to food proteins. [1][2][3][4] Currently, approaches are being developed using protein chemistry and in vitro and in silico methods to characterize food proteins and derivatives that arise during product processing and reformulation, which may explain why certain food proteins induce sensitization of the immune system, while others are tolerated. 5,6 However, elucidating the mechanisms underlying allergen sensitization is a complex, multidimensional problem that often requires a wide range of additional in vivo and ex vivo experimentation, 5 as a wide range of molecules, tissues, and cells play a role in the mechanisms underlying food allergen sensitization.…”

Section: Introductionmentioning

confidence: 99%

“…A variety of in vitro and in vivo models have been developed that address the factors and mechanisms involved in the sensitization to food proteins . Currently, approaches are being developed using protein chemistry and in vitro and in silico methods to characterize food proteins and derivatives that arise during product processing and reformulation, which may explain why certain food proteins induce sensitization of the immune system, while others are tolerated .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overview of in vivo and ex vivo endpoints in murine food allergy models: Suitable for evaluation of the sensitizing capacity of novel proteins?

Castan

Bøgh

Maryniak

et al. 2019

Allergy

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Significant efforts are necessary to introduce new dietary protein sources to feed a growing world population while maintaining food supply chain sustainability. Such a sustainable protein transition includes the use of highly modified proteins from side streams or the introduction of new protein sources that may lead to increased clinically relevant allergic sensitization. With food allergy being a major health problem of increasing concern, understanding the potential allergenicity of new or modified proteins is crucial to ensure public health protection. The best predictive risk assessment methods currently relied on are in vivo models, making the choice of endpoint parameters a key element in evaluating the sensitizing capacity of novel proteins. Here, we provide a comprehensive overview of the most frequently used in vivo and ex vivo endpoints in murine food allergy models, addressing their strengths and limitations for assessing sensitization risks. For optimal laboratory‐to‐laboratory reproducibility and reliable use of predictive tests for protein risk assessment, it is important that researchers maintain and apply the same relevant parameters and procedures. Thus, there is an urgent need for a consensus on key food allergy parameters to be applied in future food allergy research in synergy between both knowledge institutes and clinicians.

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Epithelial models to study food allergen-induced barrier disruption and immune activation

Cited by 12 publications

References 76 publications

Epithelial Barrier Dysfunction in Chronic Respiratory Diseases

Epithelial Barrier Dysfunction in Chronic Respiratory Diseases

Environmental factors in epithelial barrier dysfunction

Overview of in vivo and ex vivo endpoints in murine food allergy models: Suitable for evaluation of the sensitizing capacity of novel proteins?

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