Transcriptomic and metabolomic analyses reveal several critical metabolic pathways and candidate genes involved in resin biosynthesis in Pinus massoniana

Bai, Qingsong; He, Boxiang; Cai, Yanling; Lian, Huiming; Zhang, Qian

doi:10.1007/s00438-019-01624-1

Cited by 18 publications

(19 citation statements)

References 48 publications

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“…Transcriptome sequencing is a feasible and economical technique for generating relatively comprehensive sequencing data within a short time, and this technique has become popular in plant research 19,28 . Previous studies have demonstrated that the AP2/ERF gene family is one of the most abundant transcriptional factor families in Masson pine 19,33 . AP2/ERF TFs are thought to be involved in the response to drought stress, the response to pine wood nematode disease and the biosynthesis of resin in Masson pine, the most abundant resin-producing species worldwide, but Masson pine is also threatened by pine wood nematode disease 34,35 .…”

Section: Discussionmentioning

confidence: 99%

“…For the past few years, Masson pine clones that are highly resistant to pine wilt disease have been screened in southern China 36 . Moreover, Masson pine is a good resource for identifying genes associated with resin biosynthesis and pine wood nematode disease resistance 22,33 . However, few AP2/ERF genes have been reported in Masson pine until now, and these genes have rarely been reported across Pinaceae species.…”

Section: Discussionmentioning

confidence: 99%

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Identification, classification, and characterization of AP2/ERF superfamily genes in Masson pine (Pinus massoniana Lamb.)

Zhu

Chen

Zhang

et al. 2021

Sci Rep

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Transcription factors (TFs) play crucial regulatory roles in controlling the expression of the target genes in plants. APETALA2/Ethylene-responsive factors (AP2/ERF) are part of a large superfamily of plant-specific TFs whose members are involved in the control of plant metabolism, development and responses to various biotic and abiotic stresses. However, the AP2/ERF superfamily has not been identified systematically in Masson pine (Pinus massoniana), which is one of the most important conifer in southern China. Therefore, we performed systematic identification of the AP2/ERF superfamily using transcriptome sequencing data from Masson pine. In the current study, we obtained 88 members of the AP2/ERF superfamily. All PmAP2/ERF members could be classified into 3 main families, AP2 (7 members), RAV (7 members), ERF (73 members) families, and a soloist protein. Subcellular localization assays suggested that two members of PmAP2/ERF were nuclear proteins. Based on pine wood nematode (PWN) inoculated transcriptome and qPCR analysis, we found that many members of PmAP2/ERF could respond to PWN inoculation and PWN related treatment conditions in vitro. In general, members of the AP2/ERF superfamily play an important role in the response of Masson pine responds to PWN. Furthermore, the roles of the AP2/ERF superfamily in other physiological activities of Masson pine remain to be further studied.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification, classification, and characterization of AP2/ERF superfamily genes in Masson pine (Pinus massoniana Lamb.)

Zhu

Chen

Zhang

et al. 2021

Sci Rep

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“…Resin from Boswellia has been viewed as one of the significant defense responses to wounding and pathogenic attacks ( Cabrita, 2018 ; Trapp and Croteau, 2001 ). Resin, synthesized via methylerythritol 4-phosphate (MEP) or mevalonic acid (MVA) pathways ( Bai et al., 2019 ; Mao et al., 2019 ), is transported to wounded sites through resin canals ( Khan et al., 2018b ; Mengistu et al., 2013 ). The resin is composed of more than 300 chemical constituents ( Al-Harrasi et al., 2019a ) identified as monoterpenes (KEGG pathway: map00902) and diterpenes (KEGG pathway: map00904), and triterpenes (KEGG pathway: map00909; map01062).…”

Section: Resultsmentioning

confidence: 99%

Genome structure and evolutionary history of frankincense producing Boswellia sacra

et al. 2022

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“…Transcriptomic analysis was carried out in masson pine secondary xylem. The phenotype data of trait resin-tapping capacity (RYC) was calculated by formula described in previous study (Zeng et al, 2013;Bai et al, 2020). The masson pines carrying high (H), medium (M), and low (L) RYCs, three lines for each, were selected for RNA-seq.…”

Section: Candidate Gene Identification and Transcriptomic Analysismentioning

confidence: 99%

“…Primer 3 (version 4.1.0) was used to design the specific primers (Untergasser et al, 2012). Actin was used as reference gene to normalize the relative expression profiles (Liu et al, 2015;Bai et al, 2020). The primers of all genes were listed in Table S2.…”

Section: Validation Of Expression Levels Of the Putative Genesmentioning

confidence: 99%

Genome-wide association study of terpenoids in resin reveals candidate genes for resin yield in Pinus massoniana

Bai¹,

Zhang²,

Cai³

et al. 2020

Dendrobiology

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Masson pine (Pinus massoniana) is an important species for resin tapping in China. Molecular assisted selection (MAS) has been gradually introduced in to the selection and breeding of masson pine. Until now, the lack of connection between genome information and phenotypic data has hindered the breeding and selection of important chemical compounds of xylem resin. In this study, gas chromatography mass spectrometer (GC-MS) was employed for qualitative and quantitative analysis of chemical compounds of resin collected from 69 masson pine germplasm resources. Then, genome-wide association study (GWAS) was conducted for the terpenoids with higher heritability. A total of 15 primary compounds, including six diterpenes and nine monoterpenes/sesquiterpenes, were detected from xylem resin. By using five GWAS methods, 42 associated SNPs were identified from these masson pines. The P. taeda genome database was used for detecting candidate genes related to the associated SNPs. By using transcriptome data, several members of PKc_like super family, Cytochrome P450, AP2-EFR, F-box, and ABC transporter were found to be highly expressed in the masson pines with higher resin-yielding capacity. The terpenoids associated SNPs can be directly used for MAS. These candidate genes, especially the two F-box members, will contribute to the study of terpenoid biosynthesis mechanisms in the future.

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Transcriptomic and metabolomic analyses reveal several critical metabolic pathways and candidate genes involved in resin biosynthesis in Pinus massoniana

Cited by 18 publications

References 48 publications

Identification, classification, and characterization of AP2/ERF superfamily genes in Masson pine (Pinus massoniana Lamb.)

Identification, classification, and characterization of AP2/ERF superfamily genes in Masson pine (Pinus massoniana Lamb.)

Genome structure and evolutionary history of frankincense producing Boswellia sacra

Genome-wide association study of terpenoids in resin reveals candidate genes for resin yield in Pinus massoniana

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