Transcriptome profiling of Fusarium solani f. sp. eumartii -infected potato tubers provides evidence of an inducible defense response

D‘Ippólito, Sebastián; Martin, María Laura; Salcedo, María Florencia; Atencio, Hugo Marcelo; Casalongué, Claudia Anahí; Godoy, Andrea Verónica; Fiol, Diego Fernando

doi:10.1016/j.pmpp.2010.09.002

Cited by 23 publications

(17 citation statements)

References 55 publications

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“…Consistent with the phenotypic observations, the transgenic leaves had subdued induction of pr-1 and osm gene transcripts compared to their robust induction in the WT leaves within day 1 of pathogen inoculation. Since these genes in potato tubers form a part of hypersensitive defense response against this fungus [80], it is evident that msrA3 expression interferes with the pathogen-mediated HR. The suppressive effect of the msrA3 expression on HR induction was further supported by the pattern of induction or lack thereof of cat and apx gene transcripts in the transgenics.…”

Section: Discussionmentioning

confidence: 99%

Expression of an Engineered Heterologous Antimicrobial Peptide in Potato Alters Plant Development and Mitigates Normal Abiotic and Biotic Responses

et al. 2013

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Antimicrobial cationic peptides (AMPs) are ubiquitous small proteins used by living cells to defend against a wide spectrum of pathogens. Their amphipathic property helps their interaction with negatively charged cellular membrane of the pathogen causing cell lysis and death. AMPs also modulate signaling pathway(s) and cellular processes in animal models; however, little is known of cellular processes other than the pathogen-lysis phenomenon modulated by AMPs in plants. An engineered heterologous AMP, msrA3, expressed in potato was previously shown to cause resistance of the transgenic plants against selected fungal and bacterial pathogens. These lines together with the wild type were studied for growth habits, and for inducible defense responses during challenge with biotic (necrotroph Fusarium solani) and abiotic stressors (dark-induced senescence, wounding and temperature stress). msrA3-expression not only conferred protection against F. solani but also delayed development of floral buds and prolonged vegetative phase. Analysis of select gene transcript profiles showed that the transgenic potato plants were suppressed in the hypersensitive (HR) and reactive oxygen species (ROS) responses to both biotic and abiotic stressors. Also, the transgenic leaves accumulated lesser amounts of the defense hormone jasmonic acid upon wounding with only a slight change in salicylic acid as compared to the wild type. Thus, normal host defense responses to the pathogen and abiotic stressors were mitigated by msrA3 expression suggesting MSRA3 regulates a common step(s) of these response pathways. The stemming of the pathogen growth and mitigating stress response pathways likely contributes to resource reallocation for higher tuber yield.

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Section: Discussionmentioning

confidence: 99%

Expression of an Engineered Heterologous Antimicrobial Peptide in Potato Alters Plant Development and Mitigates Normal Abiotic and Biotic Responses

et al. 2013

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“…Generally, studies on differentially expressed genes upon infection of potato with pathogens focus on the host's response (Beyer et al 2001;D'Ippólito et al 2010;Derksen et al 2013;El-Bebany 2010;Gao et al 2013;Gyetvai et al 2012), however, no studies have been done on the down-regulation of fungal genes in these pathosystems. In our study, the high abundance of EST sequences belonging to the delta-endotoxin CytB family prompted us to characterize the corresponding cDNA sequence from R. solani AG-3 Rs114 and study its behavior under different biotic interactions.…”

Section: Carbohydrate-binding Genesmentioning

confidence: 99%

“…However, the multi-year survival of R. solani in the soil reduces the effectiveness of such practices. Currently, no resistant potato varieties are commercially available, despite global genome sequencing and functional analysis efforts of potato and other pathogens (Beyer et al 2001;Cubeta et al 2014;D'Ippólito et al 2010;Derksen et al 2013;El-Bebany 2010;Gao et al 2013;Gyetvai et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

et al. 2014

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Rhizoctonia solani is a plant pathogenic fungus that causes black scurf on tubers and stem and stolon canker on underground parts of potato plant. Early in the season, the fungus attacks germinating sprouts underground before they emerge from the soil. Damage at this stage results in delayed emergence of weakened plants with poor and uneven stands. The mechanism underlying this phenomenon has been investigated in this study by coupling a cDNA-suppression subtractive hybridization (SSH) library to differential screening to identify transcripts of R. solani that are down-regulated during infection of potato sprouts. We report on the identification of 33 unique genes with functions related to carbohydrate binding, vitamin synthesis, pathogenicity, translation, ATP and nucleic acid binding and other categories. RACE-PCR was used to clone and characterize the first full-length cDNA clones, RSENDO1 and RSGLYC1 that encode for an eukaryotic delta-endotoxin CytB protein and an intracellular glycosyl hydrolase, respectively. Quantitative real-time PCR revealed the down-regulation of RSENDO1 during infection of potato sprouts and the up-regulation of RSGLYC1 when the fungus was grown on a cellulose-based nutrient medium. In contrast, additional experiments have highlighted the down-regulation of RSENDO1 when R. solani was co-cultured with the mycoparasite Stachybotrys elegans and the bacterial antagonist Bacillus subtilis B26. These results advance our understanding of R. solani-potato interaction in subterranean parts of the plant. Such approaches could be considered in building an efficient integrated potato disease management program.

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“…RNA-Seq results typically show high levels of reproducibility for both technical and biological replicates [13,14]. During the past decade, the genes and gene networks associated with important potato tuber traits, such as the tuber formation [15][16][17], antiviral properties [18][19][20], and starch biosynthesis [21][22][23], have been identified and functionally characterized using RNA-Seq technology. So far, knowledge of plant tuberization was mainly obtained from studies in potato.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Key Pathways and Hormone Activities Involved in Early Microtuber Formation of Dioscorea opposita

Zhao

Dong

et al. 2020

BioMed Research International

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Chinese yam (Dioscorea opposita) is an important tuberous crop used for both food and medicine. Despite a long history of cultivation, the understanding of D. opposita genetics and molecular biology remains scant, which has limited its genetic improvement. This work presents a de novo transcriptome sequencing analysis of microtuber formation in D. opposita. We assembled cDNA libraries from different stages during the process of microtuber formation, designated as initial explants (EXP), axillary bud proliferation after three weeks (BUD), and microtuber visible after four weeks (MTV). More differentially expressed genes (DEGs) and pathways were identified between BUD vs. EXP than in MTV vs. BUD, indicating that proliferation of the axillary bud is the key stage of microtuber induction. Gene classification and pathway enrichment analysis showed that microtuber formation is tightly coordinated with primary metabolism, such as amino acid biosynthesis, ribosomal component biosynthesis, and starch and sucrose metabolism. The formation of the microtuber is regulated by a variety of plant hormones, including ABA. Combined with analysis of physiological data, we suggest that ABA positively regulates tuberization in D. opposita. This study will serve as an empirical foundation for future molecular studies and for the propagation of D. opposita germplasm in field crops.

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Transcriptome profiling of Fusarium solani f. sp. eumartii -infected potato tubers provides evidence of an inducible defense response

Cited by 23 publications

References 55 publications

Expression of an Engineered Heterologous Antimicrobial Peptide in Potato Alters Plant Development and Mitigates Normal Abiotic and Biotic Responses

Expression of an Engineered Heterologous Antimicrobial Peptide in Potato Alters Plant Development and Mitigates Normal Abiotic and Biotic Responses

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

Transcriptome Analysis Reveals Key Pathways and Hormone Activities Involved in Early Microtuber Formation of Dioscorea opposita

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