Transcriptome profile of human neuroblastoma cells in the hypomagnetic field

Abstract

Research has shown that the hypomagnetic field (HMF) can affect embryo development, cell proliferation, learning and memory, and in vitro tubulin assembly. In the present study, we aimed to elucidate the molecular mechanism by which the HMF exerts its effect, by comparing the transcriptome profiles of human neuroblastoma cells exposed to either the HMF or the geomagnetic field. A total of 2464 differentially expressed genes (DEGs) were identified, 216 of which were up-regulated and 2248 of which were down-regulated after exposure to the HMF. These DEGs were found to be significantly clustered into several key processes, namely macromolecule localization, protein transport, RNA processing, and brain function. Seventeen DEGs were verified by real-time quantitative PCR, and the expression levels of nine of these DEGs were measured every 6 h. Most notably, MAPK1 and CRY2, showed significant up-and down-regulation, respectively, during the first 6 h of HMF exposure, which suggests involvement of the MAPK pathway and cryptochrome in the early bio-HMF response. Our results provide insights into the molecular mechanisms underlying the observed biological effects of the HMF. hypomagnetic field, geomagnetic field, transcriptome profile, massively parallel sequencing, MAPK1, CRY2 Citation:Mo WC, Liu Y, Bartlett PF, He RQ. Transcriptome profile of human neuroblastoma cells in the hypomagnetic field.

Biological effects of the hypomagnetic field: An analytical review of experiments and theories

“…According to the data of the Table, closest to the primary physical level are studies that report on the involvement of gene expression in magnetoreception, e.g. [80, 108, 120, 123] and [139, 144, 156, 162, 166, 173], with different genes expressing in different situations. This suggests that the MF targets are rather physical and distributed, but they operate as magnetic receptors only in some specific conditions.…”

Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase

“…The HMF inhibits mitochondrial function in mouse myocardium and primary muscle cells (Fu et al, 2016; Nepomnyashchikh et al, 1997). By using transcriptome profiling, we found that the differentially expressed genes in the HMF-exposed human neuroblastoma SH-SY5Y cells were involved in the process of metabolism and oxidative stress (Mo et al, 2014). Moreover, Martino and colleagues found that the HMF reduces H 2 O 2 production in fibrosarcoma HT1080 and pancreatic AsPC-1 cancer cells (Martino and Castello, 2011).…”

Planarian regeneration in space: Persistent anatomical, behavioral, and bacteriological changes induced by space travel

“…On Earth, biological systems are also subject to the naturally varying geomagnetic field (GMF) (Dubrov, 1978). This variation in geomagnetic disturbance has been shown to impact not only animal behavior (Beischer, 1971;Zamoshchina et al, 2012), but also medically relevant phenomena such as ciliary motion (Sandoze, Svanidze, & Didimova, 1995), stem cell function (Mo, Liu, Bartlett, & He, 2014), cardiovascular regulation (Cornelissen et al, 2001;Feigin et al, 2014;Gmitrov & Gmitrova, 2004;Stoupel, 2006;Stoupel et al, 2014), the autonomic nervous system (Baevsky, Petrov, & Chernikova, 1998), memory (Wang, Xu, Li, Li, & Jiang, 2003;Xiao, Wang, Xu, Jiang, & Li, 2009;Zhang et al, 2004), and the interactions between neurons (Shibib, Brock, & Gosztony, 1987). Magnetic field reversals may even have placed selective pressures on organisms that have contributed to subsequent extinction (Hays, 1971;Plotnick, 1980) and morphological change (Harrison & Funnel, 1964), and planaria have specifically been shown to be sensitive to weak magnetic fields (Brown, 1962b.…”