Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase

Abstract

Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field (HMF), on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species (ROS) in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase (SOD) activity and expression, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect.Electronic supplementary materialThe online version of this article (doi:10.1007/s13238-017-0403-9) contains supplementary material, which is available to authorized users.

Hypomagnetic fields cause anxiety in adult male mice

“…Martino and Castello [] also verified that HMF stress suppressed intracellular hydrogen peroxide production, which has been correlated with superoxide dismutase (SOD) activity. Zhang et al [] confirmed that HMF reduces hydrogen peroxide production in human neuroblastoma cells and inhibits the activity of CuZn superoxide dismutase. Therefore, HMF can be considered a stress factor that induces oxidative stress and affects the production of reactive oxygen species (ROS).…”

The Hyperfine Coupling Radical Pair Mechanism of Biological Effects on Weak Magnetic Fields

“…For example, a HMF can significantly suppress Arabidopsis flowering in light [14]. Because of the HMF as a key environmental factor when long-term and longdistance space mission in outer space, and also in some magnetic shielding conditions on the ground,the biological effect of the HMF should be seriously considered [73]. Some studies on the mechanisms underlying the biological effects of a HMF have been carried out [6].…”

“…In Ouyang and Li [7] the author and his partner gave the point: Equation 1 may be one quantum mechanism of biological effects on a HMF. Ambient alternating fields always exist [73,74] can they influence the biological effects on a HMF ? The author thinks ambient alternating fields can influence the biological effects on a HMF just like on GMF as discussed in part 2.That is to say Equation 2 may be one possible quantum mechanism of the biological effects of a HMF when considering ambient alternating fields.…”

“…The author thinks ambient alternating fields can influence the biological effects on a HMF just like on GMF as discussed in part 2.That is to say Equation 2 may be one possible quantum mechanism of the biological effects of a HMF when considering ambient alternating fields. In Zhang, et al [73]…”

The geomagnetic field effects on animals: A review

“…(2) 自由基途径: 动物地磁导航的感光受体 自由基感磁模型认为外界磁场的变化会调控细胞内 的自由基电子自旋状态, 影响不同能级自由基产物 的数量, 例如隐花色素Cry感磁过程 [59,98] , 而体外研 究发现细胞经亚磁场处理后, 通常会引起胞内活性 氧自由基(ROS)的变化. 不同的细胞类型反应不同, 肿瘤细胞系表现胞内ROS水平的下降 [99,100] , 而原代 培养的小鼠肌肉细胞内ROS水平上升 [101] , ROS的变 化破坏了细胞正常的氧化还原平衡, 从而可能引起 与其相关的一些反应. 例如ROS过多可能引起线粒 体细胞膜的损伤, 细胞凋亡等 [102] .…”