Transcriptome and digital gene expression analysis of herbaceous peony (Paeonia lactiflora Pall.) to screen thermo-tolerant related differently expressed genes

Tao, Jun; Hao, Zhaojun; Wei, Mengran; Gong, Shusheng; Zhao, Daqiu

doi:10.1007/s13258-016-0465-8

Cited by 24 publications

(20 citation statements)

References 51 publications

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“…In this respect, the effects of high temperature stress on P. lactiflora physiology and biochemistry were investigated, and it was found that high temperature-tolerant P. lactiflora cultivars could effectively reduce stress damage by enhancing antioxidant enzyme activities and heat shock protein gene expression (Wu et al 2016). Moreover, transcriptome and digital gene expression analysis of P. lactiflora were used to screen for thermo-tolerant-related differentially expressed genes (Hao et al 2016). Additionally, Zhao et al (2015) found that shade could ameliorate high temperatureinduced growth inhibition in P. lactiflora.…”

Section: Introductionmentioning

confidence: 99%

Exogenous trehalose confers high temperature stress tolerance to herbaceous peony by enhancing antioxidant systems, activating photosynthesis, and protecting cell structure

Zhao

Hao

et al. 2019

Cell Stress and Chaperones

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Herbaceous peony (Paeonia lactiflora Pall.) is an excellent ornamental plant, which is usually stressed by summer high temperatures, but little is known about its relevant measures. In this study, the effects of trehalose on alleviating high temperature-induced damage in P. lactiflora were examined. High temperature stress in P. lactiflora increased production of reactive oxygen species (ROS), including superoxide anion free radical (O 2 •−) and hydrogen peroxide (H 2 O 2), enhanced both malondialdehyde (MDA) content and relative electrical conductivity (REC), decreased superoxide dismutase (SOD) activity, increased catalase (CAT) activity, inhibited photosynthesis, and destroyed cell structure. However, exogenous trehalose effectively alleviated its high temperature-induced damage. Trehalose decreased O 2 •− and H 2 O 2 accumulation, MDA content, and REC, increased the activities of antioxidant enzymes, enhanced photosynthesis, improved cell structure, and made chloroplasts rounder. Additionally, trehalose induced high temperaturetolerant-related gene expressions to different degrees. These results indicated that trehalose decreased the deleterious effect of high temperature stress on P. lactiflora growth by enhancing antioxidant systems, activating photosynthesis, and protecting cell structure. These findings indicate the potential application of trehalose for managing high temperatures in P. lactiflora cultivation.

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Section: Introductionmentioning

confidence: 99%

Exogenous trehalose confers high temperature stress tolerance to herbaceous peony by enhancing antioxidant systems, activating photosynthesis, and protecting cell structure

Zhao

Hao

et al. 2019

Cell Stress and Chaperones

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“…Research on the abiotic stress resistance of Paeonia has mainly focused on plant responses to low versus high temperature, drought, salt, and heavy metals. Under high-temperature (35.75/27.5°C, average daily maximum/minimum temperature) stress, heatresistant P. lactiflora 'Zifengyu' showed enhanced activity of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) and the expression of HSP, which removed reactive oxygen species effectively and prevented plants from dying 169,170 . HSP70-overexpressing transgenic plants were tolerant to high temperature 171,172 .…”

Section: Abiotic Stress Resistancementioning

confidence: 99%

Germplasm resources and genetic breeding of Paeonia: a systematic review

Yang

Sun

et al. 2020

Hortic Res

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Members of the genus Paeonia, which consists of globally renowned ornamentals and traditional medicinal plants with a rich history spanning over 1500 years, are widely distributed throughout the Northern Hemisphere. Since 1900, over 2200 new horticultural Paeonia cultivars have been created by the discovery and breeding of wild species. However, information pertaining to Paeonia breeding is considerably fragmented, with fundamental gaps in knowledge, creating a bottleneck in effective breeding strategies. This review systematically introduces Paeonia germplasm resources, including wild species and cultivars, summarizes the breeding strategy and results of each Paeonia cultivar group, and focuses on recent progress in the isolation and functional characterization of structural and regulatory genes related to important horticultural traits. Perspectives pertaining to the resource protection and utilization, breeding and industrialization of Paeonia in the future are also briefly discussed.

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“…In herbaceous peony, the focus of stress tolerance research has been on high-temperature stress and related genes. In a study by Hao et al (2016), three P. lactiflora cultivars 'Zi Feng Yu', 'Hong Yan Zheng Hui', and 'Da Fu Gui' with different thermo-tolerant abilities were selected as materials for transcriptome and digital gene expression analysis. The focus of subsequent research involved the screening of a total of 161 heat stress-responsive genes, including HSP genes.…”

Section: Genes Related To Stress Tolerancementioning

confidence: 99%

Advances in molecular biology of Paeonia L.

Fan

Wang

Dong

et al. 2019

Planta

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Molecular biology can serve as a tool to solve the limitations of traditional breeding and cultivation techniques related to flower patterns, the improvement of flower color, and the regulation of flowering and stress resistance. These characteristics of molecular biology ensured its significant role in improving the efficiency of breeding and germplasm amelioration of Paeonia. This review describes the advances in molecular biology of Paeonia, including: (1) the application of molecular markers; (2) genomics, transcriptomics, proteomics, metabolomics, and microRNA studies; (3) studies of functional genes; and (4) molecular biology techniques. This review also points out select limitations in current molecular biology, analyzes the direction of Paeonia molecular biology research, and provides advice for future research objectives.

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Transcriptome and digital gene expression analysis of herbaceous peony (Paeonia lactiflora Pall.) to screen thermo-tolerant related differently expressed genes

Cited by 24 publications

References 51 publications

Exogenous trehalose confers high temperature stress tolerance to herbaceous peony by enhancing antioxidant systems, activating photosynthesis, and protecting cell structure

Exogenous trehalose confers high temperature stress tolerance to herbaceous peony by enhancing antioxidant systems, activating photosynthesis, and protecting cell structure

Germplasm resources and genetic breeding of Paeonia: a systematic review

Advances in molecular biology of Paeonia L.

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