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BackgroundHeat shock proteins (HSPs) are found extensively in Eukaryotes and are involved in stress tolerance. However, their functions in herbaceous peony (Paeonia lactiflora Pall.) under high temperature stress are poorly characterized.ResultsIn this study, the genomic sequence of P. lactiflora HSP70, designated PlHSP70, was isolated. Its full-length was 3635 bp, and it contained a large 1440-bp intron. The encoded protein with a molecular weight of 71 kDa was localized in the cytoplasm of the cell. PlHSP70 transcription was detected in P. lactiflora and increased with the treatment of high temperature stress. The constitutive overexpression of PlHSP70 in Arabidopsis thaliana obviously conferred tolerance to high temperature stress by affecting different physiological and biochemical indices. Transgenic A. thaliana plants exhibited higher chlorophyll fluorescence values than the wild-type (WT) when exposed to high temperature stress. The accumulation of hydrogen peroxide (H2O2), superoxide anion free radical (O2·-) and relative electric conductivity (REC) were significantly lower in the transgenic A. thaliana plants compared to the WT. In addition, more intact cell membranes, chloroplasts and starch grains, and fewer plastoglobuli were found in the PlHSP70-overexpressing transgenic lines than in the WT.ConclusionsAll of these results indicated that PlHSP70 possessed the ability to improve the tolerance to high temperature in transgenic A. thaliana, which could provide a theoretical basis to improve high temperature tolerance of P. lactiflora by future genetic manipulation.Electronic supplementary materialThe online version of this article (10.1186/s12864-019-5448-0) contains supplementary material, which is available to authorized users.

Paclobutrazol (PBZ) has been associated with effects on the photosynthetic capacity of plants. PBZ affects the growth and development of plants in general. However, little is known about the effects of PBZ on photosynthetic performance and related anatomical features of herbaceous peony (Paeonia lactiflora Pall.) leaves. In the present study, PBZ application resulted in a significant reduction in peony plant height. Furthermore, PBZ application significantly increased photosynthetic rate (Pn), transpiration rate (Tr) and water use efficiency (WUE), but significantly decreased intercellular CO 2 concentration (Ci) at some stages from the bolting stage to the bud stage of the plants, compared to controls. Moreover, PBZ application increased the maximum quantum yield of PSII photochemistry (Fv/Fm), coefficient of photochemical quenching (qP) and intrinsic PSII efficiency (Φ PSII), but decreased the coefficient of non-photochemical quenching (qN) and non-photochemical quenching (NPQ). Leaves treated with PBZ had a heavy aggregation of chloroplasts close to the cell wall, with distinct grana lamellae, more and bigger starch grains (on average for a chloroplast), and fewer plastoglobuli, as compared to the control. PBZ increased chlorophyll content (SPAD) and the number of chloroplasts in individual cells in the foliar ultrastructure. PBZ-treated leaves had a darker green color with decreased luminosity (L*) and increased hue angle (h •). The results indicated that plants treated with PBZ were superior in terms of increased photosynthetic characteristics when compared with untreated controls. The direct cause of the increase in Pn and leaf greenness of PBZ-treated P. lactiflora may be the increase in chlorophyll content.

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Herbaceous peony (Paeonia lactiflora Pall.) is popular worldwide because of its gorgeous flower colour, and the yellow flower is the rarest. However, its mechanism of yellow formation is still unexplored from the post-translational level. In this study, the anatomy of the petal, cell sap pH and metal elements were investigated in bicoloured flower cultivar ‘Jinhui’ with red outer-petal and yellow inner-petal, and the yellow formation was influenced by the anatomy of petal, while not by the cell sap pH and metal elements. Subsequently, microRNAs sequencing (miRNA-seq) was used to identify small RNAs (sRNAs). A total of 4,172,810 and 3,565,152 specific unique sRNAs were obtained, 207 and 204 conserved miRNAs and 38 and 42 novel miRNAs were identified from red outer-petal and yellow inner-petal, respectively, which were confirmed by subcloning. Among these miRNAs, 163 conserved and 28 novel miRNAs were differentially expressed in two wheel of petals. And 5 differentially expressed miRNAs and their corresponding target genes related to yellow formation were screened, and their dynamic expression patterns confirmed that the yellow formation might be under the regulation of miR156e-3p-targeted squamosa promoter binding protein-like gene (SPL1). These results improve the understanding of miRNA regulation of the yellow formation in P. lactiflora.