Transcriptome analysis of the unfolded protein response in hemocytes of Litopenaeus vannamei

Chen, Yihong; Yuan, Fenghua; Bi, Haitao; Zhang, Ze-Zhi; Yue, Haitao; Yuan, Kai; Chen, Yong-Gui; Wen, Su; He, Jianguo

doi:10.1016/j.fsi.2015.10.027

Cited by 32 publications

(8 citation statements)

References 41 publications

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“…High-throughput RNA sequencing (RNA-Seq) is an efficient technology to analyze gene expression, discover transcripts, and select differentially expressed genes (DEGs) ( 20 ). This technology has been used to study the molecular basis of certain gene transcription processes ( 21 ). Ren et al found several genes related to immunity through the transcriptome profiles of M. japonicus following infection with V. parahemolyticus or WSSV ( 22 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Litopenaeus vannamei Reveals That Triosephosphate Isomerase-Like Genes Play an Important Role During Decapod Iridescent Virus 1 Infection

Liao

Wang

et al. 2020

Front. Immunol.

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Decapod iridescent virus 1 (DIV1) results in severe economic losses in shrimp aquaculture. However, little is known about the physiological effect of DIV1 infection on the host. In this study, we found that the lethal dose 50 of DIV1-infected Litopenaeus vannamei after 48, 72, 96, and 156 h were 4.86 × 10 6 , 5.07 × 10 5 , 2.13 × 10 5 , and 2.38 × 10 4 copies/μg DNA, respectively. In order to investigate the mechanisms of DIV1 infection, a comparative transcriptome analysis of hemocytes from L. vannamei , infected or not with DIV1, was conducted. The BUSCO analysis showed that the transcriptome was with high completeness (complete single-copy BUSCOs: 57.3%, complete duplicated BUSCOs: 41.1%, fragmentation: 0.8%, missing: 0.8%). A total of 168,854 unigenes were assembled, with an average length of 601 bp. Based on homology searches, Kyoto Encyclopedia of Genes and Genomes (KEGG), gene ontology (GO), and cluster of orthologous groups of proteins (KOG) analysis, 62,270 (36.88%) unigenes were annotated. Among them, 1,112 differentially expressed genes (DEGs) were identified, of which 889 genes were up-regulated and 223 genes were down-regulated after DIV1 infection. These genes were mainly annotated to the major metabolic processes such as fructose and mannose metabolism, carbon metabolism, and inositol phosphate metabolism. Among these metabolic pathways, the triosephosphate isomerase ( TPI ) family was the most eye-catching DEG as it participates in several metabolic processes. Three types of TPI, LvTPI-like, LvTPI-Blike , and LvTPI-Blike1 , were obtained for gene silencing by RNA interference. The results showed that LvTPI-like and LvTPI-Blike1 silencing caused a high mortality rate among L. vannamei . However, LvTPI-like and LvTPI-Blike silencing reduced DIV1 replication in DIV1-infected L. vannamei . All the results indicated that TPI-like genes play an important role during DIV1 infection, which provides valuable insight into the infection mechanism of DIV1 in shrimp and may aid in preventing viral diseases in shrimp culture.

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Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Litopenaeus vannamei Reveals That Triosephosphate Isomerase-Like Genes Play an Important Role During Decapod Iridescent Virus 1 Infection

Liao

Wang

et al. 2020

Front. Immunol.

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“…We confirmed that IRE1‐XBP1 pathway was activated by LPS injection. It has been reported that IRE1 and PERK induced fatty acid, phospholipid and cholesterol biosynthetic pathways (Chen et al, 2016). Moreover, ammonia treatment of Penaeus monodon caused significant changes to apoptosis‐related genes such as PERK (Li et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide induced unfolded protein response and related anti‐lipopolysaccharide factor expression in Pacific white shrimp, Litopenaeus vannamei

Wang

Ning

et al. 2021

Aquaculture Research

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Litopenaeus vannamei (L. vannamei), also known as Pacific white shrimp, is one of the largest cultured shrimp species in the world, accounting for more than 70 per cent of global shrimp production for its rapid growth, tender meat and high nutritional value (Loker et al., 2010). Unfortunately, L. vannamei is susceptible to bacteria which can lead to massive death of shrimp, and cause huge economic losses to the farming industry (Walker & Winton, 2010). Therefore, it is necessary to properly understand the pathogenesis of bacterial

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“…The results were accordance with previous studies in L . vannamei [ 90 , 91 ] and M . rosenbergii [ 92 ], suggesting that HSP21 was involved in the reponse to WSSV infection and may play an important role in anti-virus responses in M .…”

Section: Discussionmentioning

confidence: 99%

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

Zhao

Sun

et al. 2018

PLoS ONE

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White spot syndrome virus (WSSV) is one of the most devastating pathogens of cultured shrimp, responsible for massive loss of its commercial products worldwide. The oriental river prawn Macrobrachium nipponense is an economically important species that is widely farmed in China and adult prawns can be infected by WSSV. However, the molecular mechanisms of the host pathogen interaction remain unknown. There is an urgent need to learn the host pathogen interaction between M. nipponense and WSSV which will be able to offer a solution in controlling the spread of WSSV. Next Generation Sequencing (NGS) was used in this study to determin the transcriptome differences by the comparison of control and WSSV-challenged moribund samples, control and WSSV-challenged survived samples of hepatopancreas in M. nipponense. A total of 64,049 predicted unigenes were obtained and classified into 63 functional groups. Approximately, 4,311 differential expression genes were identified with 3,308 genes were up-regulated when comparing the survived samples with the control. In the comparison of moribund samples with control, 1,960 differential expression genes were identified with 764 genes were up-regulated. In the contrast of two comparison libraries, 300 mutual DEGs with 95 up-regulated genes and 205 down-regulated genes. All the DEGs were performed GO and KEGG analysis, overall a total of 85 immune-related genes were obtained and these gene were groups into 13 functions and 4 KEGG pathways, such as protease inhibitors, heat shock proteins, oxidative stress, pathogen recognition immune receptors, PI3K/AKT/mTOR pathway, MAPK signaling pathway and Ubiquitin Proteasome Pathway. Ten genes that valuable in immune responses against WSSV were selected from those DEGs to furture discuss the response of host to WSSV. Results from this study contribute to a better understanding of the immune response of M. nipponense to WSSV, provide information for identifying novel genes in the absence of genome of M. nipponense. Furthermore, large number of transcripts obtained from this study could provide a strong basis for future genomic research on M. nipponense.

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Transcriptome analysis of the unfolded protein response in hemocytes of Litopenaeus vannamei

Cited by 32 publications

References 41 publications

Comparative Transcriptome Analysis of Litopenaeus vannamei Reveals That Triosephosphate Isomerase-Like Genes Play an Important Role During Decapod Iridescent Virus 1 Infection

Comparative Transcriptome Analysis of Litopenaeus vannamei Reveals That Triosephosphate Isomerase-Like Genes Play an Important Role During Decapod Iridescent Virus 1 Infection

Lipopolysaccharide induced unfolded protein response and related anti‐lipopolysaccharide factor expression in Pacific white shrimp, Litopenaeus vannamei

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

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