Transcriptome analysis of primary podocytes reveals novel calcium regulated regulatory networks

Abstract

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Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus nonciliated cells

“…S4C). Podoplanin had previously been shown to be a cell surface marker useful for sorting of podocytes, while Prominin1 has previously been demonstrated to mark the parietal epithelial and tubular cells ( 57 , 60 – 64 ). Podoplanin + Prominin1 − cells showed >10-fold higher expression of Wt1, the master transcription factor of podocytes, than Podoplanin + Prominin1 + cells (fig.…”

“…To further study the causative functions of NSL complex-mediated transcriptional changes in podocytes, we established a primary podocyte culture system. Published primary podocyte culture systems rely on genetic tagging of the podocyte lineage by crossing Nphs2-Cre with the mTmG reporter (56)(57)(58). Because of the early onset of the phenotype in our Kansl2-pKO mice, we attempted to isolate and culture primary podocytes from 1-week-old mice before the onset of the phenotypes described earlier.…”