Transcriptome analysis of neuropeptides and G-protein coupled receptors (GPCRs) for neuropeptides in the brown planthopper Nilaparvata lugens

Tanaka, Yoshiaki; Suetsugu, Yoshitaka; Yamamoto, Kimiko; Noda, Hiroaki; Shinoda, Tetsuro

doi:10.1016/j.peptides.2013.07.027

Cited by 120 publications

(142 citation statements)

References 41 publications

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“…Based on the lack of evidence for TRHR ligands in protostomes, except in Platynereis (22), it was proposed that TRH neuropeptides have been lost in this lineage, or at least in Ecdysozoa (21)(22)(23)53). Our in silico search revealed the presence of TRH-like peptides in nematodes, arthropods, annelids, and mollusks.…”

Section: Discussionmentioning

confidence: 94%

“…In silico studies and peptidomics previously identified 31 FLP and 52 NLP precursors in C. elegans (51,52), but none of the corresponding peptides show sequence similarity to deuterostomian TRHs or were able to activate TRHR-1 in cellculture cells. Given the presence of orphan protostomian TRHR orthologs (19)(20)(21), we hypothesized that an as yet unknown family of neuropeptide precursors in these phyla might encode the cognate ligand of TRHR-1, and we performed an in-depth in silico search. Our search identified the trh-1 precursor (C30H10.6) as the most promising candidate, because this precursor shows a phylogenetic distribution similar to protostomian TRHRs and encodes two peptides with TRH-like sequence features in terms of length and biochemical properties.…”

Section: Discussionmentioning

confidence: 99%

“…TRHR orthologs were identified in the annelid Platynereis dumerilii and in the genomes and transcriptomes of mollusks, arthropods, and Caenorhabditis elegans (19)(20)(21). However, with the exception of Platynereis and molluscan EFLGamides, no ortholog of the vertebrate TRH precursor was found in any protostomian genome (21,22). Therefore, it has been proposed that the HPT axis of vertebrates,…”

Section: ) Tripeptide Trhs Most Predicted Echinoderm Trhs Are Tetrapmentioning

confidence: 99%

“…beets@kuleuven.be. including TRH, has been lost in protostomes, at least in the ecdysozoan phyla (arthropods and nematodes) (21)(22)(23)(24) 1B). We challenged receptorexpressing cells with a synthetic library of more than 250 known and predicted C. elegans peptides, but none was able to activate TRHR-1.…”

Section: Significancementioning

confidence: 99%

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Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans

Sinay

Mirabeau

Depuydt

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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In vertebrates thyrotropin-releasing hormone (TRH) is a highly conserved neuropeptide that exerts the hormonal control of thyroidstimulating hormone (TSH) levels as well as neuromodulatory functions. However, a functional equivalent in protostomian animals remains unknown, although TRH receptors are conserved in proto-and deuterostomians. Here we identify a TRH-like neuropeptide precursor in Caenorhabditis elegans that belongs to a bilaterian family of TRH precursors. Using CRISPR/Cas9 and RNAi reverse genetics, we show that TRH-like neuropeptides, through the activation of their receptor TRHR-1, promote growth in C. elegans. TRH-like peptides from pharyngeal motor neurons are required for normal body size, and knockdown of their receptor in pharyngeal muscle cells reduces growth. Mutants deficient for TRH signaling have no defects in pharyngeal pumping or isthmus peristalsis rates, but their growth defect depends on the bacterial diet. In addition to the decrease in growth, trh-1 mutants have a reduced number of offspring. Our study suggests that TRH is an evolutionarily ancient neuropeptide, having its origin before the divergence of protostomes and deuterostomes, and may ancestrally have been involved in the control of postembryonic growth and reproduction.thyrotropin-releasing hormone | C. elegans | neuropeptide | molecular evolution | growth regulation A fter Harris's initial proposal on the hypothalamic control of pituitary secretion (1), it took almost two decades to identify the first hypophysiotropic molecule. In 1969 the groups of Schally and Guillemin isolated the tripeptide pQHP-NH 2 (2, 3), named "thyrotropin-releasing hormone" (TRH). The sequence of TRH is fully conserved across all vertebrates, indicating that strong evolutionary pressure has acted to preserve its structure (4). In all vertebrate phyla TRH is synthesized from a larger precursor protein (preproTRH) that contains five to eight copies of the TRH sequence (4). Following the explosion of genome and transcriptome sequence data, preproTRH was identified in chordate species lacking a bona fide pituitary, e.g., cephalochordates (5), and in the genomes of more ancient deuterostomes, including echinoderms (6, 7). In contrast to vertebrate (pQHP-NH 2 ) and chordate (pQSP-NH 2 ) tripeptide TRHs, most predicted echinoderm TRHs are tetrapeptidesLike vertebrate TRH, they are small peptides with an N-terminal pyroglutamate, a C-terminal amide (-NH 2 ) group, and amino acids with aromatic or cyclic side chains at the second and third positions. TRH therefore is widely distributed throughout the deuterostomian lineage of the Animal Kingdom, suggesting an ancient origin for this neuropeptide hormone.In mammals, hypothalamic TRH is the prime regulator of the set point of thyroid-stimulating hormone (TSH) synthesis and secretion by the anterior pituitary thyrotrophs (9). TSH secretion stimulates the thyroid gland to produce the thyroid hormones (THs) thyroxine (T 4 ) and triiodothyronine (T 3 ). The hypothalamus-pituitary-thyroid (HPT) axis is essentia...

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: ) Tripeptide Trhs Most Predicted Echinoderm Trhs Are Tetrapmentioning

confidence: 99%

Section: Significancementioning

confidence: 99%

See 2 more Smart Citations

Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans

Sinay

Mirabeau

Depuydt

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…2). However, Tanaka et al (2014) proposed that RPRC001000 would be a thyrotropin releasing hormone receptor (THRH), closely related to FaLPR and ETHR (Tanaka et al, 2014), because it clusters with crustaceans and arachnids THRH.…”

Section: Biogenic Aminesmentioning

confidence: 99%

Identification of G protein coupled receptors for opsines and neurohormones in Rhodnius prolixus. Genomic and transcriptomic analysis

Ons

Lavore

Sterkel

et al. 2016

Insect Biochemistry and Molecular Biology

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Genome‐wide characterization and transcriptomic analyses of neuropeptides and their receptors in an endoparasitoid wasp, Pteromalus puparum

Teng

et al. 2019

Arch Insect Biochem Physiol

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In insects, neuropeptides constitute a group of signaling molecules that act in regulation of multiple physiological and behavioral processes by binding to their corresponding receptors. On the basis of the bioinformatic approaches, we screened the genomic and transcriptomic data of the parasitoid wasp, Pteromalus puparum, and annotated 36 neuropeptide precursor genes and 33 neuropeptide receptor genes. Compared to the number of precursor genes in Bombyx mori (Lepidoptera), Chilo suppressalis (Lepidoptera), Drosophila melanogaster (Diptera), Nilaparvata lugens (Hemiptera), Apis mellifera (Hymenoptera), and Tribolium castaneum (Coleoptera), P. puparum (Hymenoptera) has the lowest number of neuropeptide precursor genes. This lower number may relate to its parasitic life cycle. Transcriptomic data of embryos, larvae, pupae, adults, venom glands, salivary glands, ovaries, and the remaining carcass revealed stage‐, sex‐, and tissue‐specific expression patterns of the neuropeptides, and their receptors. These data provided basic information about the identity and expression profiles of neuropeptides and their receptors that are required to functionally address their biological significance in an endoparasitoid wasp.

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Transcriptome analysis of neuropeptides and G-protein coupled receptors (GPCRs) for neuropeptides in the brown planthopper Nilaparvata lugens

Cited by 120 publications

References 41 publications

Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans

Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans

Identification of G protein coupled receptors for opsines and neurohormones in Rhodnius prolixus. Genomic and transcriptomic analysis

Genome‐wide characterization and transcriptomic analyses of neuropeptides and their receptors in an endoparasitoid wasp, Pteromalus puparum

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