Transcriptome analysis indicates that GmAGAMOUS-Like 15 may enhance somatic embryogenesis by promoting a dedifferentiated state

Perry, Sharyn E.; Zheng, Qi; Zheng, Yumei

doi:10.1080/15592324.2016.1197463

Cited by 17 publications

(18 citation statements)

References 27 publications

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“…We also found that transgenic expression of RcAGL15 reduces IAA and GA contents, but increases CTK and ABA contents, in accordance with previous findings that AGL15 directly and indirectly controls components of the auxin signaling pathway, in both Arabidopsis and soybean in a manner, that would limit auxin signaling (Zheng et al 2016).…”

Section: Transgenic Ectopic Expression Of Rcagl15 Changes Endogenoussupporting

confidence: 92%

“…A decrease in biologically active GA caused an increase in SE, whereas addition of active GA (GA 3 ) reduced SE (Wang et al 2004). Recent studies have shown that GhAGL15s is preferentially expressed during SE and promotes embryogenic callus formation in Gossypium hirsutum (Yang et al 2014), while AGAMOUS-Like 15 may enhance SE by promoting a dedifferentiated state in Glycine max (Perry et al 2016). AGL15 negatively regulates auxin signaling in both Arabidopsis and soybean, at many levels of the pathway, PLBs are somatic embryonic structures that develop from intermediary callus, and differ in terms of developmental process, morphological characters and microscopic structure from other kinds of somatic embryos of higher plants (Tian et al 2008).…”

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confidence: 99%

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A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis

Liu

et al. 2017

Can. J. Plant Sci.

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We isolated a gene encoding a putative MADS-box protein designated RcAGL15 from PLB (Protocorm-like body, a somatic embryogenetic structure) of Rosa canina. RcAGL15 encodes a predicted protein of 251 amino acid residues with a molecular weight of 28 kDa and theoretical isoelectric point of 7.5. RT-PCR shows that RcAGL15 is mainly expressed in PLB and regenerated PLB, but not in leaves of aseptic seedlings, incubated leaflets before callus formation, calli, rhizoids or leaves of regenerated shoots. Transgenic, ectopic expression of RcAGL15 in Arabidopsis changes numbers of floral organs and reduces contents of indole-3-acetic acid, but increases levels of abscisic acid. Thus RcAGL15 appears to be a new MADS-box gene that plays regulatory roles in the morphological development and numbers of flower organ, as well as somatic embryogenesis.

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Section: Transgenic Ectopic Expression Of Rcagl15 Changes Endogenoussupporting

confidence: 92%

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confidence: 99%

A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis

Liu

et al. 2017

Can. J. Plant Sci.

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“…Overexpression of GmAGL15 in explants, as well as the induction of SE in the cv Jack wild type, both up-regulate a common set of genes that are significantly overrepresented for response to stress (Zheng and Perry, 2014). Additionally, soybean orthologs of Arabidopsis genes encoding transcription factors associated with dedifferentiation show increased transcript in 35S pro :GmAGL15 explants compared with the cv Jack wild type and in response to the induction of SE in the cv Jack wild type for 3 dac (Perry et al, 2016). Thus, 35S pro :(Gm)AGL15 may prime tissue to be more competent to 2,4-D by the expression of stress-and dedifferentiation-associated genes, yet it may allow an appropriate level of auxin signaling.…”

Section: How Does Repression Of the Auxin Response Lead To Increased Se?mentioning

confidence: 97%

Gene Regulation by the AGL15 Transcription Factor Reveals Hormone Interactions in Somatic Embryogenesis

Zheng

et al. 2016

Plant Physiol.

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The MADS box transcription factor Arabidopsis (Arabidopsis thaliana) AGAMOUS-LIKE15 (AGL15) and a putative ortholog from soybean (Glycine max), GmAGL15, are able to promote somatic embryogenesis (SE) in these plants when ectopically expressed. SE is an important means of plant regeneration, but many plants, or even particular cultivars, are recalcitrant for this process. Understanding how (Gm)AGL15 promotes SE by identifying and characterizing direct and indirect downstream regulated genes can provide means to improve regeneration by SE for crop improvement and to perform molecular tests of genes. Conserved transcription factors and the genes they regulate in common between species may provide the most promising avenue to identify targets for SE improvement. We show that (Gm)AGL15 negatively regulates auxin signaling in both Arabidopsis and soybean at many levels of the pathway, including the repression of AUXIN RESPONSE FACTOR6 (ARF6) and ARF8 and TRANSPORT INHIBITOR RESPONSE1 as well as the indirect control of components via direct expression of a microRNA-encoding gene. We demonstrate interaction between auxin and gibberellic acid in the promotion of SE and document an inverse correlation between bioactive gibberellic acid and SE in soybean, a difficult crop to transform. Finally, we relate hormone accumulation to transcript accumulation of important soybean embryo regulatory factors such as ABSCISIC ACID INSENSITIVE3 and FUSCA3 and provide a working model of hormone and transcription factor interaction in the control of SE.

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“…Previous work has shown that transient activation or ectopic expression of one or several transcription factors can trigger the transition from NEC to EC or increase the frequency of EC formation. These include WOX5 [73], WUSHEL [74], WRKY2 [75], GRD/RKD4 [76], BBM [77], LEC1 [54], FUS3 [78], ABI3 [79], and AGL15 [80]. For example, the AP2/ERF TF BBM in plants induced plentiful cell regeneration and was used to produce a large number of somatic embryos that could developed to seedlings [81].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

Parris

et al. 2020

Preprint

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• Background • Genotype independent transformation and whole plant regeneration through somatic embryogenesis relies heavily on the intrinsic ability of a genotype to regenerate. • Results • In this study, gene expression profiles of a highly regenerable Gossypium hirsutum L. cultivar, Jin668, were analyzed at two critical developmental stages during somatic embryogenesis, non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells. The rate of EC formation in Jin668 is 96%. Differential gene expression analysis revealed a total of 5,333 differentially expressed genes (DEG) with 2,534 upregulated and 2,799 downregulated in EC. A total of 144 genes were unique to NEC cells and 174 genes unique to EC. Clustering and enrichment analysis identified genes upregulated in EC that function as transcription factors/DNA binding, phytohormone response, oxidative reduction, and regulators of transcription; while genes categorized in methylation pathways were downregulated. Four key transcription factors were identified based on their sharp upregulation in EC tissue; LEAFY COTYLEDON 1 (LEC1), BABY BOOM (BBM), FUSCA (FUS3) and AGAMOUS-LIKE15 with distinguishable subgenome expression bias. • Conclusions • This comparative analysis of NEC and EC transcriptomes gives new insights into the genetic underpinnings of somatic embryogenesis in cotton.

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Transcriptome analysis indicates that GmAGAMOUS-Like 15 may enhance somatic embryogenesis by promoting a dedifferentiated state

Cited by 17 publications

References 27 publications

A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis

A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis

Gene Regulation by the AGL15 Transcription Factor Reveals Hormone Interactions in Somatic Embryogenesis

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

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