A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis

Abstract

We isolated a gene encoding a putative MADS-box protein designated RcAGL15 from PLB (Protocorm-like body, a somatic embryogenetic structure) of Rosa canina. RcAGL15 encodes a predicted protein of 251 amino acid residues with a molecular weight of 28 kDa and theoretical isoelectric point of 7.5. RT-PCR shows that RcAGL15 is mainly expressed in PLB and regenerated PLB, but not in leaves of aseptic seedlings, incubated leaflets before callus formation, calli, rhizoids or leaves of regenerated shoots. Transgenic, ectopic expression of RcAGL15 in Arabidopsis changes numbers of floral organs and reduces contents of indole-3-acetic acid, but increases levels of abscisic acid. Thus RcAGL15 appears to be a new MADS-box gene that plays regulatory roles in the morphological development and numbers of flower organ, as well as somatic embryogenesis.

In the name of the rose: a roadmap for rose research in the genome era

“…To circumvent such limitations in the meantime, some rose genes have been studied in heterologous systems, including Tobacco 131 , Pelargonium 132 and Arabidopsis 85,133–135 . Systems for transient expression in rose have been developed by agroinfiltration in petals (e.g.…”

AGL15 Promotion of Somatic Embryogenesis: Role and Molecular Mechanism

“…For example, plant transformation in soybean is accelerated when overexpression of AGL15 enhances somatic embryogenesis, which could be utilized for producing elite cultivars. Similarly, AGL15 could provide a vital role in scaling up production of valuable crops such as coffee through propagation and genetic transformation (Etienne et al, 2018 ; Xu et al, 2018 ). Other crops could benefit from examining AGL15 expression in their species, and possibly enhancing transformation strategies via better regeneration by SE.…”

“…The non-embryogenic line (A17) showed no such increase. Ghadirzadeh-Khorzoghi et al ( 2019 ) showed increased PvAGL15 transcript in embryogenic explants of pistachio, while Xu et al ( 2018 ) showed RcAGL15 associated with protocorm-like bodies in rose.…”

A Cucumber AGAMOUS-LIKE 15 (AGL15) MADS-Box Gene Mediates Abnormal Leaf Morphology in Arabidopsis

“…In addition to controlling floral transition, ectopic expression of AtAGL15 or AtAGL18 in Arabidopsis can also enhance somatic embryogenesis, affect leaf morphogenesis, reduce fertility, and delay floral organ senescence and abscission [14,16,17], indicating the redundant functions of AtAGL15 and AtAGL18 in these developmental processes. AtAGL15 orthologs have been functionally characterized in other plants, such as cotton (G. hirsutum) [18], soybean (Glycine max) [19][20][21], Rosa canina [22], and Brassica juncea [23]. In addition, AGL15 members can control diverse developmental processes by modulating the hormone signaling pathways.…”

“…For example, AtAGL15 and GmAGL15 negatively regulate auxin signaling pathway to promote somatic embryogenesis in Arabidopsis and soybean, and this regulation is integrated with GA metabolism and ethylene signaling pathway [19,20,24,25]. Overexpression of an AtAGL15 ortholog from R. canina in Arabidopsis altered floral organ morphology and numbers, as well as somatic embryogenesis, with a reduction in IAA and GA contents [22].…”