Transcriptome analysis and postharvest behavior of the kiwifruit ‘Actinidia deliciosa’ reveal the role of ethylene-related phytohormones during fruit ripening

Salazar, J.; Zapata, Patricio; González, Makarena; Pacheco, Igor; Bastías, Macarena; Meneses, Claudio; Jorquera, Claudia; Moreno, Israel; Shinya, Paulina; Infante, Rodrigo

doi:10.1007/s11295-021-01493-z

Cited by 17 publications

(9 citation statements)

References 70 publications

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“…To infer the potential functions of AcLHCs in regulating kiwifruit development and response to ethylene treatment, we re-analyzed the expression profiles of AcLHCs in different fruit developmental stages treated with or without ethylene ( Figure 3 C). The results reveal that the expression levels of five AcLHCs ( AcLhca3.1/2.1/2.2 , AcSEP2 , and AcSEP3.1 ) were significantly down-regulated and one AcLHC ( AcLhcb8 ) was down-regulated with the ethylene treatment ( Table S4 ), verifying that those six AcLHCs could respond for kiwifruit degreening caused by the ethylene treatment [ 31 ].…”

Section: Resultsmentioning

confidence: 88%

Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

Luo

Abid

et al. 2022

IJMS

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Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays a vital role in photosynthesis. However, the reported knowledge of LHCs in kiwifruit is inadequate and poorly understood. In this study, we identified 42 and 45 LHC genes in Actinidia chinensis (Ac) and A. eriantha (Ae) genomes. Phylogenetic analysis showed that the kiwifruit LHCs of both species were grouped into four subfamilies (Lhc, Lil, PsbS, and FCII). Expression profiles and qRT-PCR results revealed expression levels of LHC genes closely related to the light, temperature fluctuations, color changes during fruit ripening, and kiwifruit responses to Pseudomonas syringae pv. actinidiae (Psa). Subcellular localization analysis showed that AcLhcb1.5/3.1/3.2 were localized in the chloroplast while transient overexpression of AcLhcb3.1/3.2 in tobacco leaves confirmed a significantly increased content of chlorophyll a. Our findings provide evidence of the characters and evolution patterns of kiwifruit LHCs genes in kiwifruit and verify the AcLhcb3.1/3.2 genes controlling the chlorophyll a content.

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Section: Resultsmentioning

confidence: 88%

Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

Luo

Abid

et al. 2022

IJMS

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“…It is worth noting that two genes were upregulated, PSII core complex gene psbY (MD06G1160500) and PSII reaction center gene PSB28 (MD10G1176000). The evidence of genes that encode proteins linked to photosynthesis in the previous study as psbY is indicating that the slowdown in the degradation of chlorophylls by 1-MCP would also affect other photosystem II-related proteins [ 61 ]. PsbY gene up-regulation expression may play a critical role in photosynthetic systems under freezing stress.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated

Zhou

Liu

et al. 2021

BMC Genomics

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Background Freezing temperatures are an abiotic stress that has a serious impact on plant growth and development in temperate regions and even threatens plant survival. The wild apple tree (Malus sieversii) needs to undergo a cold acclimation process to enhance its freezing tolerance in winter. Changes that occur at the molecular level in response to low temperatures are poorly understood in wild apple trees. Results Phytohormone and physiology profiles and transcriptome analysis were used to elaborate on the dynamic response mechanism. We determined that JA, IAA, and ABA accumulated in the cold acclimation stage and decreased during freezing stress in response to freezing stress. To elucidate the molecular mechanisms of freezing stress after cold acclimation, we employed single molecular real-time (SMRT) and RNA-seq technologies to study genome-wide expression profiles in wild apple. Using the PacBio and Illumina platform, we obtained 20.79G subreads. These reads were assembled into 61,908 transcripts, and 24,716 differentially expressed transcripts were obtained. Among them, 4410 transcripts were differentially expressed during the whole process of freezing stress, and these were examined for enrichment via GO and KEGG analyses. Pathway analysis indicated that “plant hormone signal transduction”, “starch and sucrose metabolism”, “peroxisome” and “photosynthesis” might play a vital role in wild apple responses to freezing stress. Furthermore, the transcription factors DREB1/CBF, MYC2, WRKY70, WRKY71, MYB4 and MYB88 were strongly induced during the whole stress period. Conclusions Our study presents a global survey of the transcriptome profiles of wild apple trees in dynamic response to freezing stress after two days cold acclimation and provides insights into the molecular mechanisms of freezing adaptation of wild apple plants for the first time. The study also provides valuable information for further research on the antifreezing reaction mechanism and genetic improvement of M. sieversii after cold acclimation.

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“…Preharvest foliar applications to control fruit diseases using aminoethoxyvinylglycine (AVG) [ 21 , 22 ], SA [ 23 , 24 ], and chitosan [ 25 , 26 ] have also been claimed to diminish ethylene production. In this aspect, transcriptome testing (RNA sequencing) has been used as a valuable tool to study the molecular mechanism of fruit quality changes in apricot [ 2 , 27 , 28 ], persimmon [ 9 ], kiwifruit [ 29 , 30 ], pummelo [ 31 ], and banana fruit [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Yield, Fruit Quality, and Storability of ‘Canino’ Apricot in Response to Aminoethoxyvinylglycine, Salicylic Acid, and Chitosan

Elmenofy

Okba

Salama

et al. 2021

Plants

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Ethylene plays a pivotal role in the climacteric fruit ripening and senescence process. The effect of three ethylene inhibitors on the yield, quality, and storability of ‘Canino’ apricot fruit was studied. Foliar sprays of distilled water (control), aminoethoxyvinylglycine (AVG) (150 and 100 mg·L−1), salicylic acid (SA) (4 and 2 mM), and chitosan (2.5% and 1.5%) were applied 30 and 15 days before harvest. Results indicated that the high concentrations of AVG and SA recorded the lowest percentage of preharvest fruit drop and, hence, the highest yield. Trees receiving either concentration of AVG showed the highest fruit firmness. High concentrations of all three ethylene inhibitors reduced fruit weight loss, total carotenoids, and soluble solid content (SSC), but increased total acidity (TA) during cold storage (2 °C). A high score of overall taste acceptability was observed with a higher concentration of SA, which was also recorded the lowest fruit malondialdehyde content (MDA) at harvest and during storage. The highest concentrations of SA and chitosan recorded no decay for 28 days of storage. Gene expression analysis reflected higher expression of PaACS1 gene with the highest concentrations of ethylene inhibitors, suggesting that SA (4 mM) is recommended for optimal yield, quality, and storability of ‘Canino’ apricot fruit grown under Egyptian conditions.

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Transcriptome analysis and postharvest behavior of the kiwifruit ‘Actinidia deliciosa’ reveal the role of ethylene-related phytohormones during fruit ripening

Cited by 17 publications

References 70 publications

Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated

Yield, Fruit Quality, and Storability of ‘Canino’ Apricot in Response to Aminoethoxyvinylglycine, Salicylic Acid, and Chitosan

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