Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

Luo, Jingchu; Abid, Muhammad; Tu, Jing; Gao, Puxing; Wang, Zupeng; Huang, Hongwen

doi:10.3390/ijms23126528

Cited by 11 publications

(5 citation statements)

References 61 publications

(84 reference statements)

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“…Although the LHC proteins in apples vary in length, the clustered LHC proteins share the same protein motif location and gene structure. It is consistent with previous studies at LHC family in the kiwifruit [53], which indicated that this family is highly conserved during evolution. The LHC gene is more widely distributed on the apple genome and is most abundant on chr.17 (Fig.…”

Section: Discussionsupporting

confidence: 93%

The MhLHCB15 gene interacts with interacting proteins to regulate iron deficiency tolerance in Malus halliana

Dong,

Zhang,

Cheng

et al. 2024

Preprint

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Background Iron (Fe) plays a crucial role as a micronutrient in facilitating plant growth and development. Alterations in the availability of iron can trigger a response resulting in iron deficiency, ultimately affecting both plant growth and crop yield. Results A total of 33 MhLHC family members were identified in Malus halliana based on the apple genome database under Fe deficiency. The study comprehensively analyzed the physicochemical properties, gene structures, conserved motif compositions, evolutionary relationships, and chromosomal distributions of these family members. Additionally, 15 genes were selected for qRT-PCR to verify whether they responded to abiotic stress. It was demonstrated that MhLHCB15 significantly responded to Fe deficiency stress. Among them, Further investigation was conducted on the functional properties of MhLHCB15 in Arabidopsis and apple calli. Overexpression of MhLHCB15 enhanced Fe deficiency tolerance of in both Arabidopsis and apple calli, which evoked a variety of biochemical changes: transgenic Arabidopsis displayed higher photosynthetic efficiency (Tr, Gs, and Pn) and fluorescence parameters (qP, F0, Fm, and Fv/Fm) and lower Ci compared to the WT. The chlorophyll content, photosynthetic products (SS, St and SP), and enzyme activities (SOD, POD and CAT) of transgenic A. thaliana and apple calli were markedly higher compared to the WT, however, the MDA content and ROS accumulation were less than the WT. Moreover, the FCR activity of transgenic Arabidopsis and apple calli was stronger in comparison to the WT. Conclusions In summary, overexpression of the MhLHCB15 gene enhanced Arabidopsis and apple calli tolerance to Fe deficiency stress. In this study, the interaction proteins MhSGR1 and MhTHF1 of MhLHCB15 were screened, which contributes to further analyze the regulation of MhLHCB15 on Fe deficiency tolerance of M. halliana.

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Section: Discussionsupporting

confidence: 93%

The MhLHCB15 gene interacts with interacting proteins to regulate iron deficiency tolerance in Malus halliana

Dong,

Zhang,

Cheng

et al. 2024

Preprint

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“…The higher expression of PSB genes under BL than under RL was also detected in Scots pine seedlings [ 50 ]. Previous studies found that LHC gene expression directly influences chlorophyll accumulation [ 51 , 52 , 53 ]. In our study, the total chlorophyll content in leaves has a similar change trend as the gene expression levels of MeLHCA1 and MeLHCA3 after BL or RL treatment.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Morphological, Physiological, Anatomical and Molecular Responses of Cassava Seedlings to Different Light Qualities

Zhou,

Li,

Fernie

et al. 2023

IJMS

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Light quality is highly important for growth control of in vitro plant cultures. Here, we investigated the effect of blue light (BL), red light (RL) and combined red and blue light (RBL) on in vitro cassava growth. Our results indicate that RL facilitated radial elongation of cassava and increased stomatal conductance as well as glucose, sucrose, fructose and starch content in leaves and cellulose content in the stem. It also enhanced SOD and POD activities but decreased the stomatal density and chlorophyll and carotenoid content in leaves. In addition, RL leads to shorter palisade cells, denser chloroplasts and more starch granules. These phenotypic changes were inverted following BL treatment. The expression levels of photosynthesis-related genes MeLHCA1, MeLHCA3, MePSB27-2, MePSBY, MePETE1 and MePNSL2 in leaves were at their lowest following RL treatment, while the expression levels of MePSB27-2, MePSBY, MePETE1 and MePNSL2 were at their highest after BL treatment. The phenotypic changes after RBL treatment were between the values observed for the RL and BL treatments alone. Moreover, the responses of SC8 and SC9 cassava varieties to light quality were largely conserved. As such, we believe that the results of this study lay the foundation for controlling the in vitro growth of cassava seedlings by light quality.

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“…Light-harvesting chlorophyll a/b-binding (LHC) proteins play indispensable roles in capturing solar energy during photosynthesis, photoprotection of photosystem II (PSII) and alleviation of oxidative stress caused by stress conditions ( de Bianchi et al., 2008 ; Luo et al., 2022 ). To study their contributions to low temperature stress, we identified a total of 45 LpLhc superfamily proteins in the L. perenne genome ( Table S12 ).…”

Section: Discussionmentioning

confidence: 99%

Combined genomic and transcriptomic analysis reveals the contribution of tandem duplication genes to low-temperature adaptation in perennial ryegrass

Wang

Fan

et al. 2023

Front. Plant Sci.

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Perennial ryegrass (Lolium perenne L.) is an agronomically important cool-season grass species that is widely used as forage for ruminant animal production and cultivated in temperate regions for the establishment of lawns. However, the underlying genetic mechanism of the response of L. perenne to low temperature is still unclear. In the present study, we performed a comprehensive study and identified 3,770 tandem duplication genes (TDGs) in L. perenne, and evolutionary analysis revealed that L. perenne might have undergone a duplication event approximately 7.69 Mya. GO and KEGG pathway functional analyses revealed that these TDGs were mainly enriched in photosynthesis, hormone-mediated signaling pathways and responses to various stresses, suggesting that TDGs contribute to the environmental adaptability of L. perenne. In addition, the expression profile analysis revealed that the expression levels of TDGs were highly conserved and significantly lower than those of all genes in different tissues, while the frequency of differentially expressed genes (DEGs) from TDGs was much higher than that of DEGs from all genes in response to low-temperature stress. Finally, in-depth analysis of the important and expanded gene family indicated that the members of the ELIP subfamily could rapidly respond to low temperature and persistently maintain higher expression levels during all low temperature stress time points, suggesting that ELIPs most likely mediate low temperature responses and help to facilitate adaptation to low temperature in L. perenne. Our results provide evidence for the genetic underpinning of low-temperature adaptation and valuable resources for practical application and genetic improvement for stress resistance in L. perenne.

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Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content

Cited by 11 publications

References 61 publications

The MhLHCB15 gene interacts with interacting proteins to regulate iron deficiency tolerance in Malus halliana

The MhLHCB15 gene interacts with interacting proteins to regulate iron deficiency tolerance in Malus halliana

Integrated Analysis of Morphological, Physiological, Anatomical and Molecular Responses of Cassava Seedlings to Different Light Qualities

Combined genomic and transcriptomic analysis reveals the contribution of tandem duplication genes to low-temperature adaptation in perennial ryegrass

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