Transcriptional Profiling Reveals Novel Interactions between Wounding, Pathogen, Abiotic Stress, and Hormonal Responses in Arabidopsis,

Abstract

Mechanical wounding not only damages plant tissues, but also provides pathways for pathogen invasion. To understand plant responses to wounding at a genomic level, we have surveyed the transcriptional response of 8,200 genes in Arabidopsis plants. Approximately 8% of these genes were altered by wounding at steady-state mRNA levels. Studies of expression patterns of these genes provide new information on the interactions between wounding and other signals, including pathogen attack, abiotic stress factors, and plant hormones. For example, a number of wound-responsive genes encode proteins involved in pathogen response. These include signaling molecules for the pathogen resistance pathway and enzymes required for cell wall modification and secondary metabolism. Many osmotic stress-and heat shock-regulated genes were highly responsive to wounding. Although a number of genes involved in ethylene, jasmonic acid, and abscisic acid pathways were activated, many in auxin responses were suppressed by wounding. These results further dissected the nature of mechanical wounding as a stress signal and identified new genes that may play a role in wounding and other signal transduction pathways.

MYB transcription factor genes as regulators for plant responses: an overview

“…The family of R2R3-MYB-like transcription factors has repeatedly been implicated in JAdependent defense responses. For instance, the OsLTR1 gene from rice regulated JA-dependent defense whereas AtMYB15, AtMYB34, AtMYB51 and AtMYB75 were associated with the wound response or resistance against insect herbivores (Cheong et al 2002;Johnson and Dowd 2004). The BOTRYTIS SUSCEPTIBLE 1(BOS1) gene encoded an R2R3 MYB transcription factor protein which was found to be involved in biotic as well as abiotic stress response.…”

Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses

“…Transcriptome analyses have generated considerable data, which show extensive overlapping on gene expression between biotic and abiotic stresses. Many of these overlapped genes encode signaling components, including transcription factors and protein kinases [5][6][7][8][9].…”

Expression Profiles in Barley Microspore Embryogenesis

“…A multi-dimensional stress response was observed, since genes related to water deficit, osmotic stress, oxidative stress, phosphate starvation, cold stress, and response to wounding and pathogens were found. This is in agreement with the existence of complex networks in abiotic and biotic stress responses (Cheong et al 2002). Among these stress-response genes, genes encoding glutathione S-transferases (GSTs) were overrepresented, representing a total of 2% of the up-regulated genes.…”