Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone

Tonchev, Anton B.; Yamashima, Tetsumori; Sawamoto, Kazunobu

doi:10.1016/j.neuroscience.2006.01.053

Cited by 21 publications

(18 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the central nervous system, Olig2 has been implicated in regulating the multipotent neural progenitors that lie in the subventricular zone of the adult rat brain (Buffo et al, 2005; Takemura, 2005). Under stressed conditions, these neural progenitor cells begin proliferating and expressing both Olig2 and Pax6; however, Olig2 is expressed in the neuronal precursors and not the proliferative precursors in higher mammals (Hack et al, 2004; Tonchev et al, 2006). In mouse, Olig2 expression was strongly upregulated in glial cells in response to a stab wound to the brain and inhibition of Olig2 expression forced these cells to prematurely differentiate (Buffo, 2007; Buffo et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration

Thummel

Kassen

Enright

et al. 2008

Experimental Eye Research

182

199

View full text Add to dashboard Cite

The adult zebrafish retina exhibits a robust regenerative response following light-induced photoreceptor cell death. This response is initiated by the Müller glia proliferating in the inner nuclear layer (INL), which gives rise to neuronal progenitor cells that continue to divide and migrate to the outer nuclear layer (ONL), where they differentiate into rod and cone photoreceptors. We previously conducted a microarray analysis of retinal gene expression at 16, 31, 51, 68, and 96 hours of constant intense-light treatment to identify genes and their corresponding proteins that may be involved in the generation and proliferation of the neuronal progenitor cells. We examined the expression of two candidate transcription factors, Pax6 and Ngn1, and one candidate transgene, olig2:EGFP, in the regenerating light-damaged retina. We compared the temporal and spatial expression patterns of these markers relative to PCNA (proliferating cell nuclear antigen), an established marker for proliferating cells in the zebrafish retina, and the Tg(gfap:EGFP) nt11 transgenic line that specifically labels Müller glial cells. We found that Müller glial cells dedifferentiate during regeneration, based on the loss of cell-specific markers such as GFAP (glial fibrillary acidic protein) and glutamine synthetase following their reentry into the cell cycle to produce neuronal progenitors. Pax6 expression was first detected in the proliferating neuronal progenitors by 51 hours of constant light treatment, which is significantly after the Müller glia first reenter the cell cycle after 31 hours of light. This suggests that Pax6 expression increases in neuronal progenitors, rather than in the proliferating Müller glia. EGFP expression from the olig2 promoter was first detected by 68 hours of constant light treatment in the dedifferentiated Müller glia, with Pax6 expressed in the closely-associated proliferating neuronal progenitors migrating to the ONL. Both Pax6 and olig2 expression persisted until three days post light-treatment, when the neuronal progenitors begin differentiating into new rod and cone photoreceptors. Ngn1 protein expression was initially detected in proliferating neuronal progenitors at 68 hours of light treatment. However, Ngn1 expression persisted in a subset of the INL nuclei until 17 days post-light treatment. Using the Tg(gfap:EGFP) nt11 transgenic line, Ngn1 was localized to the Müller glial nuclei that were reestablished following the regenerative response. These markers, therefore, can be used to identify different cell types at particular stages of retinal regeneration: neuronal progenitor formation, proliferation, and the reestablishment of the Müller glia cells. These markers will be important to further characterize the regeneration response in other

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration

Thummel

Kassen

Enright

et al. 2008

Experimental Eye Research

182

199

View full text Add to dashboard Cite

show abstract

“…In contrast to mammals, long-lasting progenitors display a radial morphology in the adult brains of birds, reptiles and fishes. (5,23,26) It remains (49,51,(66)(67)(68)(69) Similar to mammals, in zebrafish, the neurogenic cascades controlling the commitment and differentiation of progenitors towards a neuronal fate seem to share common molecular signals in embryonic and postembryonic stages. These include the general involvement of Notch and bHLH proneural and neurogenic factors.…”

Section: Multipotentiality Of Rodent and Zebrafish Adult Neural Progementioning

confidence: 99%

Adult neurogenesis in non‐mammalian vertebrates

Chapouton¹,

Jagasia²,

2007

View full text Add to dashboard Cite

Adult neurogenesis is an exciting and rapidly advancing field of research. It addresses basic biological questions, such as the how and why of de novo neuronal production during adulthood, as well as medically relevant issues, including the potential link between adult neural stem cells and psychiatric disorders, or how stem cell manipulation might be used as a strategy for neuronal replacement. Current research mainly focuses on rodents, but we review here recent examination of non‐mammalian vertebrates, which demonstrates that bona fide adult neural stem cells exist in these species. Importantly, especially in teleost fish, these cells can be abundant and located in various brain areas. Hence, non‐mammalian vertebrate species provide invaluable comparative material for extracting core mechanisms of adult neural stem cell maintenance and fate. BioEssays 29:745–757, 2007. © 2007 Wiley Periodicals, Inc.

show abstract

“…Our previous study demonstrated that at 28 days after hypoxia treatment, myelinated axons in the cerebral cortex, hippocampus and lateral ventricle of rats were visible in the normal group, but not obvious in the hypoxia group[37]. Another study showed that expression of various transcription factors, such as Olig1, was upregulated in the subependymal region of adult mammals after cerebral ischemia, and participated in the ischemic pathological process[38]. Hypoxia and ischemia mediated nerve demyelination.…”

Section: Discussionmentioning

confidence: 99%

Negative regulation of miRNA-9 on oligodendrocyte lineage gene 1 during hypoxic-ischemic brain damage

2014

View full text Add to dashboard Cite

Oligodendrocyte lineage gene 1 plays a key role in hypoxic-ischemic brain damage and myelin repair. miRNA-9 is involved in the occurrence of many related neurological disorders. Bioinformatics analysis demonstrated that miRNA-9 complementarily, but incompletely, bound oligodendrocyte lineage gene 1, but whether miRNA-9 regulates oligodendrocyte lineage gene 1 remains poorly understood. Whole brain slices of 3-day-old Sprague-Dawley rats were cultured and divided into four groups: control group; oxygen-glucose deprivation group (treatment with 8% O2 + 92% N2 and sugar-free medium for 60 minutes); transfection control group (after oxygen and glucose deprivation for 60 minutes, transfected with control plasmid) and miRNA-9 transfection group (after oxygen and glucose deprivation for 60 minutes, transfected with miRNA-9 plasmid). From the third day of transfection, and with increasing culture days, oligodendrocyte lineage gene 1 expression increased in each group, peaked at 14 days, and then decreased at 21 days. Real-time quantitative PCR results, however, demonstrated that oligodendrocyte lineage gene 1 expression was lower in the miRNA-9 transfection group than that in the transfection control group at 1, 3, 7, 14, 21 and 28 days after transfection. Results suggested that miRNA-9 possibly negatively regulated oligodendrocyte lineage gene 1 in brain tissues during hypoxic-ischemic brain damage.

show abstract

Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone

Cited by 21 publications

References 57 publications

Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration

Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration

Adult neurogenesis in non‐mammalian vertebrates

Negative regulation of miRNA-9 on oligodendrocyte lineage gene 1 during hypoxic-ischemic brain damage

Contact Info

Product

Resources

About