Negative regulation of miRNA-9 on oligodendrocyte lineage gene 1 during hypoxic-ischemic brain damage

Abstract

Oligodendrocyte lineage gene 1 plays a key role in hypoxic-ischemic brain damage and myelin repair. miRNA-9 is involved in the occurrence of many related neurological disorders. Bioinformatics analysis demonstrated that miRNA-9 complementarily, but incompletely, bound oligodendrocyte lineage gene 1, but whether miRNA-9 regulates oligodendrocyte lineage gene 1 remains poorly understood. Whole brain slices of 3-day-old Sprague-Dawley rats were cultured and divided into four groups: control group; oxygen-glucose deprivation group (treatment with 8% O2 + 92% N2 and sugar-free medium for 60 minutes); transfection control group (after oxygen and glucose deprivation for 60 minutes, transfected with control plasmid) and miRNA-9 transfection group (after oxygen and glucose deprivation for 60 minutes, transfected with miRNA-9 plasmid). From the third day of transfection, and with increasing culture days, oligodendrocyte lineage gene 1 expression increased in each group, peaked at 14 days, and then decreased at 21 days. Real-time quantitative PCR results, however, demonstrated that oligodendrocyte lineage gene 1 expression was lower in the miRNA-9 transfection group than that in the transfection control group at 1, 3, 7, 14, 21 and 28 days after transfection. Results suggested that miRNA-9 possibly negatively regulated oligodendrocyte lineage gene 1 in brain tissues during hypoxic-ischemic brain damage.

The role of hypoxia in stem cell regulation of the central nervous system: From embryonic development to adult proliferation

“…136 HIF-1α-induced oligodendrocyte lineage gene-1 expression promotes the growth of oligodendrocytes and triggers the repair of hypoxic-induced neuronal myelin damage. 137…”

“…HIF signaling activated‐patient‐derived iPSCs transplanted into the brain during the subacute phase of white matter stroke induced the proliferation and remyelination of endogenous oligodendrocyte precursors and promoted axonal budding and, thus, cognitive recovery effects 136 . HIF‐1α‐induced oligodendrocyte lineage gene‐1 expression promotes the growth of oligodendrocytes and triggers the repair of hypoxic‐induced neuronal myelin damage 137 …”