Transcription Factor FOXO3a Is a Negative Regulator of Cytotoxicity of Fusarium mycotoxin in GES-1 Cells

Yang, Yunxia; Yu, Song; Liu, Na; Xu, Haibin; Gong, Yunyun; Wu, Yongning; Wang, Peilong; Su, Xiaoou; Liao, Yu-Cai; Saeger, Sarah De; Humpf, Hans‐Ulrich; Wu, Aibo

doi:10.1093/toxsci/kfy216

Cited by 12 publications

(16 citation statements)

References 35 publications

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“…The inhibition rate reached 80% at all concentrations after 72 hours of DON treatment. Similar experimental phenomena have also been confirmed in the study by Yang et al [56]. In addition, Dai’s [57] research also demonstrated that DON not only causes dose-dependent cytotoxicity, but also causes time-dependent cytotoxicity of mouse endometrial stromal cells.…”

Section: Resultssupporting

confidence: 77%

The Degradation of Deoxynivalenol by Using Electrochemical Oxidation with Graphite Electrodes and the Toxicity Assessment of Degradation Products

Xiong

Zhao

et al. 2019

Toxins

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Deoxynivalenol (DON) is a common mycotoxin, which is known to be extremely harmful to human and livestock health. In this study, DON was degraded by electrochemical oxidation (ECO) using a graphite electrode and NaCl as the supporting electrolyte. The graphite electrode is advantageous due to its electrocatalytic activity, reusability, and security. The degradation process can be expressed by first-order kinetics. Approximately 86.4% of DON can be degraded within 30 min at a potential of 0.5 V. The degradation rate reached 93.2% within 30 min, when 0.5 V potential was used for electrocatalyzing a 10 mg/L DON solution. The degradation rate of DON in contaminated wet distiller’s grain with solubles (WDGS) was 86.37% in 60 min. Moreover, results from the cell counting kit-8 (CCK-8) and 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining assay indicated that ECO reduced the DON-induced cytotoxicity and apoptotic bodies in a gastric epithelial cell line (GES-1) compared to the DON-treated group. These findings provide new insights into the application of ECO techniques for degrading mycotoxins, preventing food contamination, and assessing DON-related hazards.

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Section: Resultssupporting

confidence: 77%

The Degradation of Deoxynivalenol by Using Electrochemical Oxidation with Graphite Electrodes and the Toxicity Assessment of Degradation Products

Xiong

Zhao

et al. 2019

Toxins

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“…NFKB is also reported to modulate HIF-1α expression, the main transcription factor activated by low oxygen concentrations [29]. Another transcription factor, FOXO3a, plays a crucial role in DON toxicity, both through its documented role in the regulation of steroidogenesis as well as its regulation of apoptosis [30]. Accordingly, we evaluated the expression of RelA , FOXO3A, and HIF-1α as well as ERK and JNK.…”

Section: Resultsmentioning

confidence: 99%

“…DON was previously reported to modulate HIF-1α expression in the process of oxidative stress in chickens [36] and constitutively modulates the expression of NFΚB in the inhibition of cancer cell growth [37]. In human gastric epithelial cells, the toxicity of DON was associated with the modulation of apoptosis regulated by FOXO3a [30]. Our results are consistent with these reports and present a significant increase in the expression of RelA , HIF-1α, and FOXO3a after exposure of PCa cells to DON.…”

Section: Discussionmentioning

confidence: 99%

Deoxynivalenol Modulates the Viability, ROS Production and Apoptosis in Prostate Cancer Cells

Habrowska-Górczyńska

Kowalska

Urbanek

et al. 2019

Toxins

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Deoxynivalenol (DON), known as vomitoxin, a type B trichothecene, is produced by Fusarium. DON frequently contaminates cereal grains such as wheat, maize, oats, barley, rye, and rice. At the molecular level, it induces ribosomal stress, inflammation and apoptosis in eukaryotic cells. Our findings indicate that DON modulates the viability of prostate cancer (PCa) cells and that the response to a single high dose of DON is dependent on the androgen-sensitivity of cells. DON appears to increase reactive oxygen species (ROS) production in cells, induces DNA damage, and triggers apoptosis. The effects of DON application in PCa cells are influenced by the mitogen-activated protein kinase (MAPK) and NFΚB- HIF-1α signaling pathways. Our results indicate that p53 is a crucial factor in DON-associated apoptosis in PCa cells. Taken together, our findings show that a single exposure to high concentrations of DON (2–5 µM) modulates the progression of PCa.

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“…The gastrointestinal tract is often an important target for first exposure to these dietary toxins [ 30 , 31 ]. It is reported that FB1 can affect the health of the gastrointestinal tract (GIT) in animals [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells

Jia

Liu

et al. 2020

IJMS

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Fumonisin contaminates food and feed extensively throughout the world, causing chronic and acute toxicity in human and animals. Currently, studies on the toxicology of fumonisins mainly focus on fumonisin B1 (FB1). Considering that FB1, fumonisin B2 (FB2) and fumonisin B3 (FB3) could coexist in food and feed, a study regarding a single toxin, FB1, may not completely reflect the toxicity of fumonisin. The gastrointestinal tract is usually exposed to these dietary toxins. In our study, the human gastric epithelial cell line (GES-1) was used as in vitro model to evaluate the toxicity of fumonisin. Firstly, we found that they could cause a decrease in cell viability, and increase in membrane leakage, cell death and the induction of expression of markers for endoplasmic reticulum (ER) stress. Their toxicity potency rank is FB1 > FB2 >> FB3. The results also showed that the synergistic effect appeared in the combinations of FB1 + FB2 and FB1 + FB3. Nevertheless, the combinations of FB2 + FB3 and FB1 + FB2 + FB3 showed a synergistic effect at low concentration and an antagonistic effect at high concentration. We also found that myriocin (ISP-1) could alleviate the cytotoxicity induced by fumonisin in GES-1 cells. Finally, this study may help to determine or optimize the legal limits and risk assessment method of mycotoxins in food and feed and provide a potential method to block the fumonisin toxicity.

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Transcription Factor FOXO3a Is a Negative Regulator of Cytotoxicity of Fusarium mycotoxin in GES-1 Cells

Cited by 12 publications

References 35 publications

The Degradation of Deoxynivalenol by Using Electrochemical Oxidation with Graphite Electrodes and the Toxicity Assessment of Degradation Products

The Degradation of Deoxynivalenol by Using Electrochemical Oxidation with Graphite Electrodes and the Toxicity Assessment of Degradation Products

Deoxynivalenol Modulates the Viability, ROS Production and Apoptosis in Prostate Cancer Cells

Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells

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