Transcript expression of mitochondria related genes is correlated with bovine oocyte selection by BCB test

Opiela, Jolanta; Lipiński, Daniel; Słomski, Ryszard; Kątska-Książkiewicz, L.

doi:10.1016/j.anireprosci.2009.07.007

Cited by 34 publications

(27 citation statements)

References 18 publications

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“…In contrast with a previous study in which we found higher abundance of DNMT1 in BCBþ oocytes (Wei et al 2011), in the present study we found significantly lower DNMT1 expression in Ex oocytes, which is against all odds because maternal nuclear DNMT1 is required for the maintenance of methylation imprints in preimplantation embryos during the first two cell cycles (Wei et al 2011). Similarly, COX1, a respiratory chain protein encoded by mtDNA (Scarpulla 1997), has been suggested to be vital for bovine oocyte developmental competence (May-Panloup et al 2005;Opiela et al 2009a). However, expression of COX1 was lower in oocytes of higher viability (Ex oocytes) in the present study, which is in agreement with results of our recent study , implying lower abundance in fully grown compared with growing oocytes.…”

Section: Correlation Between Zp Characteristics and Developmental Capcontrasting

confidence: 99%

Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression

Mohammadi‐Sangcheshmeh

Held

Rings

et al. 2014

Reprod. Fertil. Dev.

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In the present study, equine oocytes were classified into groups of presumably high and low developmental competence according to cumulus morphology, as well as oocyte ability to metabolise brilliant cresyl blue (BCB) stain. All oocytes were evaluated individually in terms of morphometry, zona pellucida birefringence (ZPB) and relative abundance of selected candidate genes. Oocytes with an expanded cumulus (Ex), representing those with presumably high developmental competence, had a significantly thicker zona (18.2 vs 17.3µm) and a significantly higher ZPB (64.6 vs 62.1) than oocytes with a compacted cumulus (Cp). Concurrently, oocytes classified as highly developmentally competent (BCB+) had a significantly thicker zona (18.8 vs 16.1µm) and significantly higher ZPB (63.1 vs 61.3) compared with oocytes classified as having low developmental competence. Expression of TFAM, STAT3 and CKS2 was significantly higher in Ex compared with Cp oocytes, whereas expression of COX1, ATPV6E and DNMT1 was lower. Together, the data reveal that developmentally competent equine oocytes are larger in size, have higher ZPB values and exhibit a typical genetic signature of maternally derived transcripts compared with oocytes with lower in vitro developmental competence.

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Section: Correlation Between Zp Characteristics and Developmental Capcontrasting

confidence: 99%

Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression

Mohammadi‐Sangcheshmeh

Held

Rings

et al. 2014

Reprod. Fertil. Dev.

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“…Oocytes selection using BCB test is a useful method to categorize competent oocytes. It has been reported that BCB+ oocytes have significantly higher mRNA level of genes involved in mitochondrial biogenesis, showing that this may be one of the factors for their high developmental competence [28].…”

Section: Discussionmentioning

confidence: 99%

Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining

Zare

Farahani

Salehi

et al. 2015

J Assist Reprod Genet

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“…Oocytes selected by BCB did not show any differences in RE in any of the studied genes, in spite of the higher embryo development observed in BCBC oocytes. However, in bovine oocytes, Opiela et al (2010) found higher RE of the COX1 gene in immature BCBC with respect to BCBK and Torner et al (2008) showed a higher RE of S100A10 in matured BCBK than BCBC oocytes. During IVM in prepubertal sheep oocytes, we found a significant decreasing mRNA RE in S100A10 in BCBC oocytes.…”

Section: Discussionmentioning

confidence: 99%

Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep

Catalá¹,

Izquierdo²,

Uzbekova³

et al. 2011

Reproduction

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The aim of this study was to test the Brilliant Cresyl Blue (BCB) stain to select prepubertal sheep oocytes for in vitro blastocyst production. Oocyte diameter, mitochondrial activity, maturation-promoting factor (MPF) activity and mRNA relative expression (RE) of genes related to metabolism (ATPase Na C /K C transporting a 1 (ATP1A1) and cytochrome c oxidase subunit 1 (COX1)) and constitutive function of the cell (cytoplasmic polyadenylation-element-binding protein (CPEB) and S100A10) were assessed. Immature oocytes were exposed to different BCB concentrations (13, 26, 39 and 52 mM) and classified according to their cytoplasm colouration as grown BCBC (blue cytoplasm) and growing BCBK (colourless cytoplasm). Staining oocytes with 13 mM BCB during 60 min allows selection of (BCBC) the largest (123.66 mm) and most competent oocytes to develop to the blastocyst stage (21%) with a higher number of cells (69.71G6.19S.E.M.) compared with non-stained BCBK oocytes (106.82 mm, 9% and 45.91G3.35 S.E.M. respectively). Mitochondrial activity, assessed by MitoTracker Orange CMTMRos probe, was significantly higher in BCBC than in BCBK oocytes after in vitro maturation (3369 and 1565 AU respectively). MPF activity was assessed by CDC2 kinase activity assay showing significantly higher activity at metaphase II stage in BCBC than in BCBK oocytes (1.479G0.09 and 1.184G0.05 optical density respectively). The genes analysed in this work, ATP1A1, COX1, CPEB and S100A10, did not show significant effect in mRNA RE between BCB selected oocytes. In conclusion, BCB stains larger and more competent oocytes to develop to the blastocyst stage with more active mitochondria and MPF activity and higher blastocyst cell number.

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Transcript expression of mitochondria related genes is correlated with bovine oocyte selection by BCB test

Cited by 34 publications

References 18 publications

Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression

Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression

Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining

Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep

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