Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining

Zare, Zohreh; Farahani, Reza Masteri; Salehi, Mohammad; Piryaei, Abbas; Novin, Marefat Ghaffari; Fathabadi, Fatemeh Fadaei; Mohammadi, Moslem; Dehghani-Mohammadabadi, Maryam

doi:10.1007/s10815-015-0430-5

Cited by 40 publications

(35 citation statements)

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“…In the present study, treatment of culture medium with TQ was shown to reduce ROS generation and enhance GSH production (Figure ), which, in turn, had profound effects on the fertilization rate. Such results have previously been shown with L‐carnitine, melatonin, resveratrol and cysteine supplementation in culture media (De Matos & Furnus, ; Nikmard et al, ; Wang et al, ; Zare et al, ). It has also been shown that TQ could reduce oxidative stress levels via its modulatory role on NF‐κb signaling (Arif et al, ).…”

Section: Discussionsupporting

confidence: 75%

“…results are indicating that the treatment of culture medium with 10 μM-TQ does not change the cyclin B expression, but it significantly increases transcription levels of Mapk and Cdk1 genes which result to an increase of polar body extrusion in treated oocytes. These observations are consistent with previous findings, that the upregulation of Cdk1 transcript levels during IVM supplementation with L-carnitine significantly improved MPF activity at MII stage oocytes(Zare et al, 2015).…”

supporting

confidence: 93%

“…Therefore duration time was gradually increased to 22–24 hr, which the highest maturation rate was seen in the oocytes. Thus in this study likewise the other reports, (Hosseini et al, ; Jafarzadeh et al, ; Junk, Dharmarajan, & Yovich, ; Zare et al, ), GV oocytes were incubated for 22–24 hr during IVM. After this time, the expansion rate was evaluated before any manipulations, and then the oocytes were detached from cumulus cells by mechanical pipetting for evaluation of nuclear maturation.…”

Section: Methodssupporting

confidence: 75%

“…The transcription levels of some specific genes are considered as essential biomarkers to evaluate the oocyte development. It has been reported that gene expression from GV to GV breakdown in the oocytes, could be changed by undesirable and favorable alterations in culture medium (Zare et al, ). Thus during IVM, we investigated the effects of TQ on oocyte maturation, by assessing the relative abundance of three critical maternal mRNA transcripts ( Cdk1, Cyclin B and Mapk ), related to nuclear maturation (Figure a).…”

Section: Discussionmentioning

confidence: 99%

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Thymoquinone reduces intracytoplasmic oxidative stress and improves epigenetic modification in polycystic ovary syndrome mice oocytes, during in‐vitro maturation

Eini

Bidadkosh

Nazarian

et al. 2019

Molecular Reproduction Devel

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Although in‐vitro maturation (IVM) of oocytes has been presented as an alternative treatment to traditional stimulated in‐vitro fertilization, the culture condition can be improved by natural antioxidants. Thus, we investigated the protective effect of Thymoquinone (TQ) during IVM in the polycystic ovary syndrome (PCOS) mice model. The induction of PCOS was made by dehydroepiandrosterone via subcutaneous injection, in prepubertal female B6D2F1‐mice. After 21 days later, germinal vesicle (GV)‐stage‐oocytes were extracted and incubated in IVM media containing 0, 1.0, 10.0, and 100.0 μM of TQ. To assess fertilization and blastulation rates, after 22–24 hr, the treated oocytes were fertilized in‐vitro with epididymal spermatozoa. Some other oocytes were evaluated for maturation, epigenetic, and oxidative stress markers. Similarly, the mRNA expression of epigenetic enzymes genes (Dnmt1 and Hdac1), three maternally derived genes (Mapk, CyclinB, and Cdk1) and apoptosis‐related genes (Bax and Bcl2) were assessed. Our results showed that the maturation, fertilization, and blastulation rates were significantly higher in the 10.0 μM TQ‐treated group compared with the untreated group and likewise with in‐vivo matured oocytes. The Bax expression was reduced in 10.0 μM TQ matured oocytes, but Bcl2, Dnmt1, Hdac1, Cdk1, and Mapk were upregulated in this group compared to other groups. Furthermore, dimethylation of histone‐3 at lysine‐9 (H3K9m2) and DNA methylation were significantly increased whereas H4K12 acetylation (H4K12ac) was decreased in the 10.0 μM TQ‐treated group in comparison with control and in‐vivo matured oocytes. Therefore, our results are suggesting that 10.0 μM TQ may enhance the developmental competence of PCOS oocytes via the modulation of oxidative stress and epigenetic alterations.

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Section: Discussionsupporting

confidence: 75%

supporting

confidence: 93%

Section: Methodssupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Thymoquinone reduces intracytoplasmic oxidative stress and improves epigenetic modification in polycystic ovary syndrome mice oocytes, during in‐vitro maturation

Eini

Bidadkosh

Nazarian

et al. 2019

Molecular Reproduction Devel

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“…Thus, l-Cn, whose role is to deliver long-chain fatty acids to mitochondria for catabolism, was shown to decrease LB in the cells, which indirectly could indicate an increase in the energetic status of the cells. Indeed, it has been suggested that l-Cn ameliorates maturation of mouse oocytes and early embryo development (Zare et al 2015), while decreasing DNA damage and improving the in vitro blastocyst development rate in mouse embryos (Abdelrazik et al 2009). It could, therefore, be hypothesized that in the presence of l-Cn, zygotes with limited energetic reserves could not proceed to development and thus only healthy embryos could reach the stage of 2-cells.…”

Section: Discussionmentioning

confidence: 99%

l-Carnitine affects preimplantation embryo development toward infertility in mice

et al. 2016

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Abstractl-Carnitine (l-Cn), despite the beneficial role as energy-generating substance delivering long-chain fatty acids to the β-oxidation pathway in mitochondria, has been accused to cause an endometriosis-like state to BALB/c mice manifested by increased inflammatory cytokines in serum and peritoneal fluid, accumulation of immune cells in the peritoneal cavity and uterine walls and most importantly, correlating to infertility. Exploring this type of infertility, the effect of l-Cn on preimplantation embryo development, ovarian integrity and systemic maternal immunity was studied. Using nonlinear microscopy analysis, which was shown to be a powerful tool for determining embryo quality by quantitatively estimating the lipid body (LB) content of the cells, it was shown that in vitro and in vivo administration of l-Cn significantly decreased LB mean area in zygotes. Daily intraperitoneal administration of 2.5 mg l-Cn for 3, 4 and 7 days to mice significantly decreased the percent of normal zygotes. However, only the 7-day treatment persisted by affecting 2-and 8-cell stage embryos, while almost abolishing blastocyst development. Such effects were accompanied by abnormal ovarian histology, showing increased numbers of corpora luteus and elevated progesterone concentration in the serum. In addition, it was shown that the 7-day l-Cn treatment pushed maternal systemic immunity toward inflammation and immunosuppression by increasing CD11b-, CD25-and CD11bGr1-positive cells in spleen, which opposed the necessity for immunostimulation at these early stages of pregnancy. In conclusion, the results presented here demonstrated that elevated doses of l-Cn affect early stages of embryo development, leading to infertility.Reproduction (2016) 152 283-291

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Biological roles of l‐carnitine in oocyte and early embryo development

Liu

Weng

et al. 2021

Molecular Reproduction Devel

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Poor oocyte quality is responsible for female infertility. Multiple studies have been carried out to find supplements to enhance oocyte quality and mitigate infertility problems. L-carnitine and its derivatives have diverse roles in developing oocytes and early embryos. This review focuses on the in vitro and in vivo studies that using L-carnitine alone or in combination with other supplements for oocyte quality enhancement. The key roles of L-carnitine in oocyte quality and embryo growth were summarized, and the underlying mechanism was also elucidated. L-carnitine helps in the lipid metabolism process by controlling the transfer of fatty acids to mitochondria for β-oxidation. L-carnitine modulates glucose metabolism and enhances respiratory chain enzyme activity. Furthermore, it acts as an antioxidant to prevent oxidative damage and inhibit apoptosis, a signal in response to oxidative stress. Results show the potential of L-carnitine as a potential agent in assisted reproductive technology to improve oocyte quality and the subsequent embryonic development.cryopreservation, embryo development, in vitro maturation, in vivo models, L-Carnitine, oocyte quality | INTRODUCTIONOocyte developmental potential is associated with its capacity to successfully resume meiosis, be fertilized, implanted in the uterus, and finally develop into a healthy offspring (Dunning et al., 2014). Poor oocyte quality is one of the major causes of decline in female fertility. To date, enormous nutrients and pharmaceutical drugs have been proposed to enhance oocyte quality and attenuate reproductive disorders, such as different types of hormones, proteins, growth factors, and antioxidants. However, adverse effects and incidences have been reported on other systems (Rutkowski & Grzegorczyk, 2012). Considering this point, several experimental studies have been carried out in vivo or in vitro to

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Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining

Cited by 40 publications

References 54 publications

Thymoquinone reduces intracytoplasmic oxidative stress and improves epigenetic modification in polycystic ovary syndrome mice oocytes, during in‐vitro maturation

Thymoquinone reduces intracytoplasmic oxidative stress and improves epigenetic modification in polycystic ovary syndrome mice oocytes, during in‐vitro maturation

l-Carnitine affects preimplantation embryo development toward infertility in mice

Biological roles of l‐carnitine in oocyte and early embryo development

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