Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression

Mohammadi‐Sangcheshmeh, Abdollah; Held, Eva; Rings, F.; Ghanem, Nasser; Salilew‐Wondim, Dessie; Tesfaye, Dawit; Sieme, Harald; Schellander, K.; Hoelker, M.

doi:10.1071/rd12303

Cited by 11 publications

(9 citation statements)

References 70 publications

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“…This may explain the increased expression of COX1, CYTB, ND2, and ND4 in cumulus cells of R5. A study showed that the expression of COX1 is higher in oocytes with compacted cumulus than in oocytes with expanded cumulus 33 . When cell lines containing cumulus cells are cultured in serum-starvation medium, mitochondrial viability is dramatically higher than that in cells cultured under control conditions 34 .…”

Section: Discussionmentioning

confidence: 99%

Repeated superovulation may affect mitochondrial functions of cumulus cells in mice

Xie

Wang

Zhang

et al. 2016

Sci Rep

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Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely affects the mitochondrial functions of cumulus cells remains unclear. In this study, mice were divided into three groups: superovulation once (R1); superovulation three times (R3), and superovulation five times (R5). We evaluated the effects of repeated superovulation on mitochondrial DNA copies (mtDNA) and observed decreased mtDNA copies per cell with increasing number of superovulation cycles. Further, we investigated the DNA methylation status in exon 2 and the mRNA expression level of nuclear-encoded DNA polymerase gamma A (PolgA). The results showed that the DNA methylation levels of PolgA in R1 and R5 were slightly lower than in R3. Additionally, the altered DNA methylation in PolgA coincided with the changes in PolgA expression in cumulus cells. We also found that the mRNA expression of COX1, CYTB, ND2, and ND4 was altered by repeated superovulation in cumulus cells. Thus, repeated superovulation had adverse effects on mitochondrial function.

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Section: Discussionmentioning

confidence: 99%

Repeated superovulation may affect mitochondrial functions of cumulus cells in mice

Xie

Wang

Zhang

et al. 2016

Sci Rep

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“…Currently, in vitro manipulation and maturation of cumulus-oocyte complexes constitute the main channel to produce assisted reproductive technology-ready oocytes in mammals. To augment the developmental competence of oocytes cultured in vitro, a variety of techniques and methods have been pursued, such as the brilliant cresyl blue (BCB) 3 test, zona imaging, optimization of culture system, and screening of biomarkers (3,4).…”

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confidence: 99%

Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation

Liu

Wang

Liu

et al. 2018

Journal of Biological Chemistry

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The brilliant cresyl blue (BCB) test is used in both basic biological research and assisted reproduction to identify oocytes likely to be developmentally competent. However, the underlying molecular mechanism targeted by the BCB test is still unclear. To explore this question, we first confirmed that BCB-positive porcine oocytes had higher rates of meiotic maturation, better rates of cleavage and development into blastocysts, and lower death rates. Subsequent single-cell transcriptome sequencing on porcine germinal vesicle (GV)-stage oocytes identified 155 genes that were significantly differentially expressed between BCB-negative and BCB-positive oocytes. These included genes such as ,, ,, ,, and , which are enriched in functionally important signaling pathways including cell cycle regulation, oocyte meiosis, spliceosome formation, and nucleotide excision repair. In BCB-positive GV oocytes that additionally had a lower frequency of DNA double-strand breaks, the CDC5L protein was significantly more abundant./CDC5L inhibition by short interference (si)RNA or antibody microinjection significantly impaired porcine oocyte meiotic maturation and subsequent parthenote development. Taken together, our single-oocyte sequencing data point to a potential new role for CDC5L in porcine oocyte meiosis and early embryo development, and supports further analysis of this protein in the context of the BCB test.

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“…Recently several studies [8][9][10][11][12] including our own [13][14][15][16], tried to introduce and highlight the usefulness of a test called brilliant cresyl blue (BCB) staining test for screening the most competent oocytes. The test is based on glucose-6-phosphate dehydrogenase (G6PDH) activity during oocyte development.…”

Section: Introductionmentioning

confidence: 99%

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Abazari-Kia

Mohammadi‐Sangcheshmeh

Dehghani-Mohammadabadi

et al. 2013

J Assist Reprod Genet

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Purpose To associate glucose-6-phosphate dehydrogenase (G6PDH) activity in goat oocytes with intracellular glutathione (GSH) content, meiotic competence, developmental potential, and relative abundance of Bax and Bcl-2 genes transcripts. Methods Goat oocytes were exposed to brilliant cresyl blue (BCB) staining test and categorized into BCB + (bluecytoplasm), and BCB − (colorless-cytoplasm) groups. A group of oocytes were not exposed to BCB test and was considered as a control group. After maturation in vitro, a group of oocytes were used for determination of nuclear status and intracellular GSH content while another group was subjected to parthenogenetic activation followed by in vitro embryo culture.Results We found that BCB + oocytes not only yielded higher rate of maturation, but also showed an increased level of intracellular GSH content than BCB − and control oocytes.Furthermore, BCB + oocytes produced more blastocysts than BCB − and control oocytes. Our data revealed that the expression of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) genes were interacted with G6PDH-activity in mature oocyte, their surrounding cumulus cells, and blastocyst-stage embryos.Conclusions The results of this study demonstrate that selection of goat oocytes based on G6PDH-activity through the BCB test improves their developmental competence, increases intracellular GSH content, and affects the expression of the apoptosis-related genes.

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Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression

Cited by 11 publications

References 70 publications

Repeated superovulation may affect mitochondrial functions of cumulus cells in mice

Repeated superovulation may affect mitochondrial functions of cumulus cells in mice

Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

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