Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Abazari-Kia, Amir Hossein; Mohammadi‐Sangcheshmeh, Abdollah; Dehghani-Mohammadabadi, Maryam; Jamshidi-Adegani, Fatemeh; Veshkini, Abbas; Zhandi, Mahdi; Çınar, Mehmet Ulaş; Salehi, Mohammad

doi:10.1007/s10815-013-0159-y

Cited by 31 publications

(20 citation statements)

References 45 publications

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“…Highly developmental competent goat BCB+ oocytes have previously been shown to increase the intracellular GSH content compared with BCB-(with low developmental competence) and control oocytes (Abazari-Kia et al, 2014). The results of the present study indicated enhancement of oocytes glutathione content linearly with increasing concentrations of royal jelly supplemented to IVM medium, which subsequently increased maturation rate.…”

Section: Discussionsupporting

confidence: 54%

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Veshkini¹,

Mohammadi‐Sangcheshmeh²,

Ghanem³

et al. 2018

Anim Reprod

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Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes was enhanced when RJ was added to IVM medium at any supplementation compared with control. Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration of 5 mg/ml than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different in the control and RJtreated group at 5 mg/ml. However, number of apoptotic cells per blastocyst was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile of Bax, and p53 was down-regulated while Bcl-2 was upregulated in oocytes treated with RJ at 5 and 10 mg/ml compared with the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production through increasing maturation rate. RJ seems to improve the IVM microenvironment by reducing expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of apoptosis in blastocysts.

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Section: Discussionsupporting

confidence: 54%

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Veshkini¹,

Mohammadi‐Sangcheshmeh²,

Ghanem³

et al. 2018

Anim Reprod

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“…Therefore, we examined the expression of BCL2 in this study. BCL2, as an antiapoptotic gene, has also been reported as a potential target of WT1 (Loeb 2006) and it is expressed in oocytes and embryos (Abazari-Kia et al 2014). We have found that the level of anti-apoptotic gene BCL2 was downregulated at every preimplantation stage of WT1 downregulated group, which suggests that the apoptosis induced by WT1 knockdown is mediated through WT1 in porcine oocytes and embryos BCL2 down-regulation.…”

Section: Wt1 In Porcine Oocytes and Embryosmentioning

confidence: 63%

Effects of WT1 down-regulation on oocyte maturation and preimplantation embryo development in pigs

et al. 2014

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The Wilms' tumour 1 (WT1) gene originally identified as a tumour suppressor associated with WTs encodes a zinc finger-containing transcription factor that is expressed in multiple tissues and is an important regulator of cellular and organ growth, proliferation, development, migration and survival. However, there is a deficiency of data regarding the expression and function of WT1 during oocyte maturation and preimplantation embryonic development. Herein, we sought to define the expression characteristics and functions of WT1 during oocyte maturation and preimplantation embryonic development in pigs. We show that WT1 is expressed in porcine oocytes and at all preimplantation stages in embryos generated by ICSI. We then evaluated the effects of down-regulating WT1 expression at germinal vesicle and early ICSI stages using a recombinant plasmid (pGLV3-WT1-shRNA). Down-regulation of WT1 did not affect oocyte maturation but significantly decreased preimplantation embryonic development and increased apoptosis in blastocysts. These results indicate that WT1 plays important roles in the development of porcine preimplantation embryos.

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“…Several efforts have been made to find connections between the characteristics of caprine oocytes and their apoptosis incidence as well as the influence of the latter on embryonic development. It has been shown that the diameter of healthy oocytes correlates with the incidence of apoptosis (Anguita et al, 2009), follicle size correlates with DNA fragmentation in oocytes (Romaguera et al, 2010), and glucose-6-phosphate dehydrogenase (G6PDH) activity of goat oocytes (determined by brilliant cresyl blue staining) enhances embryonic development and increases Bcl-2 expression in blastocysts (Abazari-Kia et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Use of retinoids during oocyte maturation diminishes apoptosis in caprine embryos

Conceição

Moura

Ferreira-Silva

et al. 2015

Acta Veterinaria Hungarica

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Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mechanisms governing this phenomenon have not been characterised. The aim of the present study was to evaluate if the incidence of apoptosis is affected by the addition of retinyl acetate (RAc) and 9-cis-retinoic acid (RA) during in vitro maturation (IVM) of caprine oocytes. Embryonic development was recorded on days 3 and 8 post-fertilisation, and apoptosis was measured by caspase activity and DNA fragmentation (TUNEL assay). Control zygotes had lower capacity to cleave and reach the blastocyst stage (24.45 ± 2.32 and 5.32 ± 0.81, respectively) than those of RAc-(29.96 ± 1.62 and 7.94 ± 0.93, respectively) and RA-treated groups (30.12 ± 1.51 and 7.36 ± 1.02, respectively). Oocytes and blastocysts positive for TUNEL assay were more frequent, respectively, in the controls (8.20 ± 0.78, 8.70 ± 1.05) than in RAc (5.60 ± 0.52, 4.80 ± 0.51) and RA (6.40 ± 0.69, 5.40 ± 0.69). Caspase activity did not differ between control oocytes (7.20 ± 0.91), RAc (6.60 ± 0.68) and RA (7.30 ± 0.67), but it was reduced in RAc-(5.05 ± 0.62) and RA-treated blastocysts (5.75 ± 0.22) compared to controls (8.35 ± 0.71). These results indicate that the addition of retinoids during IVM increases the developmental potential of goat embryos with a concomitant reduction in apoptosis rates.

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Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Cited by 31 publications

References 45 publications

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Effects of WT1 down-regulation on oocyte maturation and preimplantation embryo development in pigs

Use of retinoids during oocyte maturation diminishes apoptosis in caprine embryos

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