Transcranial direct current stimulation alleviates seizure severity in kainic acid-induced status epilepticus rats

Wu, Yuan-Hua; Chien, Miao Er; Huang, Chih Hsu; Chiang, Chia Chu; Lin, Chou Ching K.; Durand, Dominique M.

doi:10.1016/j.expneurol.2020.113264

Cited by 11 publications

(22 citation statements)

References 52 publications

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“…The chronic rat model of spontaneous seizures following KAinduced SE was adopted in this study [20,23,24] (Supplementary Methods). Four weeks after KA-induced SE, chronic spontaneous seizures and interictal spikes appeared [25], and the rats were subjected to the tDCS experiment.…”

Section: Chronic Rat Model Of Ka-induced Spontaneous Seizuresmentioning

confidence: 99%

“…Animals were placed on the stereotaxic apparatus under anesthesia to implant a plastic cannula (1 cm-height, 1 mm-inner radius) fixed on the skull, with the cannula center located 3 mm posterior to the Bregma on the central sagittal fissure above the dorsal hippocampus. The cannula was filled with 0.9% normal saline to serve as a plugin site for the c-tDCS electrode pin [20,26,27]. An EKG electrode (30 Â 22 mm 2 pad, Ambu/NF50K) was placed at the dorsal shoulder to serve as a-tDCS electrode and fixed with conductive gel during stimulation [13].…”

Section: Depth Eeg Implantation and Tdcs Assemblymentioning

confidence: 99%

“…Rats were subjected to tDCS (1 mA, 30 min per day) or sham (1 mA, 30 s per day) for five consecutive days. This setting has been shown to alleviate seizure severity in SE rats [20] and chosen for our study. Experiments were performed under identical isoflurane gas anesthesia to reduce stress and ensure stability during stimulation, and all procedures were identical in both groups.…”

Section: Tdcs Stimulation and Eeg Recording Protocolmentioning

confidence: 99%

“…Interictal spikes were automatically detected using the following criteria: (1) peak amplitude > 10 times the baseline amplitude and > 1 mV, and (2) width at the half maximum of the peak < 200 ms with an inter-peak-interval to the prior detected spike > 70 ms [20]. The baseline amplitude for each rat was calculated by averaging the absolute amplitude values from three segments of 2-min EEGs starting at 0, 20, and 40 min from the 1-h EEG.…”

Section: Spike Detectionmentioning

confidence: 99%

“…Understanding how tDCS modulates oscillations and affects neuronal excitability in the epileptic brain is important to determine tDCS' mechanism of action. Ictal EEG oscillations have been shown to be altered in tDCS-treated status epilepticus (SE) rats [20]. Low frequency stimulation was reported to reduce seizures by deep brain stimulation [21] and optogenetic stimulation [22].…”

Section: Introductionmentioning

confidence: 99%

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Delta oscillation underlies the interictal spike changes after repeated transcranial direct current stimulation in a rat model of chronic seizures

Chien

Chiang

et al. 2021

Brain Stimulation

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Background: Transcranial direct current stimulation (tDCS) provides a noninvasive polarity-specific constant current to treat epilepsy, through a mechanism possibly involving excitability modulation and neural oscillation. Objective: To determine whether EEG oscillations underlie the interictal spike changes after tDCS in rats with chronic spontaneous seizures. Methods: Rats with kainic acid-induced spontaneous seizures were subjected to cathodal tDCS or sham stimulation for 5 consecutive days. Video-EEG recordings were collected immediately pre-and poststimulation and for the subsequent 2 weeks following stimulation. The acute pre-post stimulation and subacute follow-up changes of interictal spikes and EEG oscillations in tDCS-treated rats were compared with sham. Ictal EEG with seizure behaviors, hippocampal brain-derived neurotrophic factor (BDNF) protein expression, and mossy fiber sprouting were compared between tDCS and sham rats. Results: Interictal spike counts were reduced immediately following tDCS with augmented delta and diminished beta and gamma oscillations compared with sham. Cathodal tDCS also enhanced delta oscillations in normal rats. However, increased numbers of interictal spikes with a decrease of delta and theta oscillations were observed in tDCS-treated rats compared with sham during the following 2 weeks after stimulation. Resuming tDCS suppressed the increase of interictal spike activity. In tDCS rats, hippocampal BDNF protein expression was decreased while mossy fiber sprouting did not change compared with sham. Conclusions: The inverse relationship between the changes of delta oscillation and interictal spikes during tDCS on and off stimulation periods indicates that an enhanced endogenous delta oscillation underlies the tDCS inhibitory effect on epileptic excitability.

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