Tracking lipid profiles of Jatropha curcas L. seeds under different pruning types and water managements by low-field and HR-MAS NMR spectroscopy

Santos, Otávio Neto Almeida; Folegatti, Marcos Vinícius; Dutra, Lívia Macedo; Andrade, Irineu Pedro de Sousa; Júnior, Eder Duarte Fanaya; Lena, Bruno Patias; Barison, Andersson; Santos, Alan Diego da Conceição

doi:10.1016/j.indcrop.2017.09.066

Cited by 5 publications

(3 citation statements)

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“…biopsies) and monitoring treatment in brain, prostate, breast, and cervical cancers . Moreover, it has been especially used in the analysis of foodstuffs, such as the identification of lipids, carbohydrates, and small metabolites . This technique has several advantages in metabolomic studies, as it permits plant genotypes to be differentiated such as genetically modified organisms, different phenotypes, and chemotaxonomy identification .…”

Section: Introductionmentioning

confidence: 99%

“…5,6 Moreover, it has been especially used in the analysis of foodstuffs, such as the identification of lipids, carbohydrates, and small metabolites. 3,[7][8][9][10] This technique has several advantages in metabolomic studies, as it permits plant genotypes to be differentiated such as genetically modified organisms, 11 different phenotypes, 12,13 and chemotaxonomy identification. 4,14,15 HR-MAS NMR is also widely used for quality control, such as determining the purity, authenticity, and origin of several plant-based products.…”

Section: Introductionmentioning

confidence: 99%

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Important issues in plant tissues analyses by HR‐MAS NMR

Flores

Martinelli

Pinto

et al. 2018

Phytochemical Analysis

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Important issues in plant tissues analyses by HR‐MAS NMR

Flores

Martinelli

Pinto

et al. 2018

Phytochemical Analysis

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“…The HR-MAS NMR-based metabolomics studies in plants have been used for a wide range of applications. The influences of biotic and abiotic stress on the metabolic profile in plants has been widely studied by HR-MAS NMR [ 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ]. Metabolomics can help in understanding developmental processes, like fruit ripening.…”

Section: Applications Of Hr-mas Nmr In Plant Metabolomicsmentioning

confidence: 99%

HR-MAS NMR Applications in Plant Metabolomics

2021

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Metabolomics is used to reduce the complexity of plants and to understand the underlying pathways of the plant phenotype. The metabolic profile of plants can be obtained by mass spectrometry or liquid-state NMR. The extraction of metabolites from the sample is necessary for both techniques to obtain the metabolic profile. This extraction step can be eliminated by making use of high-resolution magic angle spinning (HR-MAS) NMR. In this review, an HR-MAS NMR-based workflow is described in more detail, including used pulse sequences in metabolomics. The pre-processing steps of one-dimensional HR-MAS NMR spectra are presented, including spectral alignment, baseline correction, bucketing, normalisation and scaling procedures. We also highlight some of the models which can be used to perform multivariate analysis on the HR-MAS NMR spectra. Finally, applications of HR-MAS NMR in plant metabolomics are described and show that HR-MAS NMR is a powerful tool for plant metabolomics studies.

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