Evaluation of Lipoxygenase Activity in Common Beans by UV and NMR Spectroscopy: Proposal for a Complementary Technique for Enzymatic Studies

Silva, Andressa Kuhnen; Oliveira, Gerlon de Almeida Ribeiro; Bassinello, P. Z.; Lanna, Anna Cristina; Novotny, E. H.; Lião, Luciano M.

doi:10.1007/s12161-019-01499-3

Cited by 5 publications

(1 citation statement)

References 33 publications

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“…According to our previous study that the linolic acid group could be oxidized by lipoxygenase to yield linoleic acid hydroperoxide, LA-OPA was subjected to the oxidization, and the generation of the corresponding LAOOH-OPA was monitored by the absorption spectrum and the thiobarbituric acid (TBA) assay . In the presence of lipoxygenase, the unsaturated linoleic acid was oxidated and the conjugated diene would be generated, with a characteristic absorption at 235 nm in UV–vis spectrum . As shown in Figure S3 of the absorption spectrum, the peak around 235 nm was observed, suggesting the successful production of LAHP.…”

Section: Resultsmentioning

confidence: 99%

Self-Deliverable Peptide-Mediated and Reactive-Oxygen-Species-Amplified Therapeutic Nanoplatform for Highly Effective Bacterial Inhibition

Huang

Yao

et al. 2021

ACS Appl. Mater. Interfaces

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An "antibiotic-free strategy" provides a viable option to address bacterial infections, especially for the "superbug" challenge. However, the undesirable antibacterial activity of antibiotic-free agents hinders their practical applications. In this study, we developed a combination antibacterial strategy of coupling peptide-drug therapy with chemodynamic therapy (CDT) to achieve the effective bacterial inhibition. An amphiphilic oligopeptide (LAOOH-OPA) containing a therapeutic unit of D (KLAK) 2 peptide and a hydrophobic linoleic acid hydroperoxide (LAHP) was designed. The positively charged D (KLAK) 2 peptide with an α-helical conformation enabled rapid binding with microbial cells via electrostatic interaction and subsequent membrane insertion to deactivate the bacterial membrane. When triggered by Fe 2+ , moreover, LAHP could generate singlet oxygen ( 1 O 2 ) to elicit lipid bilayer leakage for enhanced bacteria inhibition. In vitro assays demonstrated that the combination strategy possessed excellent antimicrobial activity not only merely toward susceptible strains (Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli) but also toward methicillinresistant Staphylococcus aureus (MRSA). On the mouse skin abscess model induced by S. aureus, self-assembled LAOOH-OPA exhibited a more significant bacteria reduction (1.4 log 10 reduction) in the bioburden compared to that of the standard vancomycin (0.9 log 10 reduction) without apparent systemic side effects. This combination antibacterial strategy shows great potential for effective bacterial inhibition.

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Section: Resultsmentioning

confidence: 99%