Tracing Effects of Na+ on Morphology, Phosphate Metabolism and Accumulated Compounds of a Sugar Beet Root by NMR.

Kano, Hiromi; Ishida, Noriko; Takagishi, Hidejiro; Shirata, Kazuto; Koizumi, Masaru

doi:10.1626/jcs.62.95

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…This finding suggests that trehalose maintains a higher degree of turgor in the petals than do the other sugars. Additionally, TNBT staining, which reflects tissue viability (Iwaya-Inoue et al, 1998;Kano et al, 1993;Rosa and Tsou, 1961), indicated that the parenchyma tissue adjacent to the vascular bundles of the florets was alive in the petals from the trehalose-treated spikes, but not in the control (Fig. 5).…”

Section: Discussionmentioning

confidence: 98%

Trehalose Delays Senescence in Cut Gladiolus Spikes

Otsubo¹,

Iwaya‐Inoue

2000

HortSci

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Florets of cut gladiolus (Gladiolus ×grandiflora hort. cv. Fujinoyuki) spikes kept at 25 °C under 14 h light/10 h dark condition showed severe wilting 4 days after flower opening. Treatment with 0.1 m trehalose prolonged vase-life 2 days, whereas inhibitors and other sugars had no effect. The upper florets also opened properly in trehalose-treated spikes, but not in cycloheximide-treated ones. After 4 days, the first florets of trehalose-treated spikes maintained water content more effectively than did controls or spikes treated with other sugars. The parenchyma adjacent to vascular bundles in the petals of trehalose-treated spikes maintained viability for 4 days. These results suggest that trehalose preserves cell viability in gladiolus spikes, thereby enhancing water uptake into petal tissues.

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Section: Discussionmentioning

confidence: 98%

Trehalose Delays Senescence in Cut Gladiolus Spikes

Otsubo¹,

Iwaya‐Inoue

2000

HortSci

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“…In the past 2 decades, some research has attempted to follow the sodium uptake in plant systems by 23 Na NMR spectroscopy, e.g., in cell suspensions (2-4) and in excised tissue (5)(6)(7)(8). Most of these studies were done with the aid of paramagnetic shift reagents (2)(3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

“…Most of these studies were done with the aid of paramagnetic shift reagents (2)(3)(4)(5)(6)(7)(8). By adding paramagnetic shift reagents (9) to the suspending medium, the intra-and extracellular fractions of the sodium signal can be separated.…”

Section: Introductionmentioning

confidence: 99%

23Na and 1H NMR Microimaging of Intact Plants

Olt

Krötz

Komor

et al. 2000

Journal of Magnetic Resonance

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“…In plant sciences, applications range from imaging of fruits, seeds, roots or shoot structures (Fedotov et al, 1969 ; Brown et al, 1986 ; Kuchenbrod et al, 1995 ; Köckenberger et al, 2004 ; Van As et al, 2009 ; Borisjuk et al, 2012 ) to measuring water status or water flow in plant organs (Köckenberger, 2001 ; Windt et al, 2006 ). Pioneering work on storage organs has been conducted on sugar beet (Kano et al, 1993 ; Macfall and Johnson, 1994 ) and Zantedeschia tuber (Robinson et al, 2000 ), which both were excavated prior to MRI measurements. Here, we employed MRI to monitor belowground storage organs of sugar beet in pots up to 117 mm inner diameter for which appropriate MRI instruments and protocols had to be established.…”

Section: Introductionmentioning

confidence: 99%

Belowground plant development measured with magnetic resonance imaging (MRI): exploiting the potential for non-invasive trait quantification using sugar beet as a proxy

et al. 2014

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Both structural and functional properties of belowground plant organs are critical for the development and yield of plants but, compared to the shoot, much more difficult to observe due to soil opacity. Many processes concerning the belowground plant performance are not fully understood, in particular spatial and temporal dynamics and their interrelation with environmental factors. We used Magnetic Resonance Imaging (MRI) as a noninvasive method to evaluate which traits can be measured when a complex plant organ is monitored in-vivo while growing in the soil. We chose sugar beet (Beta vulgaris ssp. vulgaris) as a model system. The beet consists mainly of root tissues, is rather complex regarding tissue structure and responses to environmental factors, and thereby a good object to test the applicability of MRI for 3D phenotyping approaches. Over a time period of up to 3 months, traits such as beet morphology or anatomy were followed in the soil and the effect of differently sized pots on beet fresh weight calculated from MRI data was studied. There was a clear positive correlation between the pot size and the increase in fresh weight of a sugar beet over time. Since knowledge of the development of internal beet structures with several concentric cambia, vascular and parenchyma rings is still limited, we consecutively acquired 3D volumetric images on individual plants using the MRI contrast parameter T2 to map the development of rings at the tissue level. This demonstrates that MRI provides versatile protocols to non-invasively measure plant traits in the soil. It opens new avenues to investigate belowground plant performance under adverse environmental conditions such as drought, nutrient shortage, or soil compaction to seek for traits of belowground organs making plants more resilient to stress.

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Tracing Effects of Na+ on Morphology, Phosphate Metabolism and Accumulated Compounds of a Sugar Beet Root by NMR.

Cited by 6 publications

References 32 publications

Trehalose Delays Senescence in Cut Gladiolus Spikes

Trehalose Delays Senescence in Cut Gladiolus Spikes

23Na and 1H NMR Microimaging of Intact Plants

Belowground plant development measured with magnetic resonance imaging (MRI): exploiting the potential for non-invasive trait quantification using sugar beet as a proxy

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