23Na and 1H NMR Microimaging of Intact Plants

Olt, Silvia; Krötz, Eva; Komor, Ewald; Rokitta, Markus; Haase, Axel

doi:10.1006/jmre.2000.2076

Cited by 24 publications

(12 citation statements)

References 21 publications

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“…8). This phenomenon had also been observed by 23Na NMR imaging, following the feeding of sodium salts to plants (Olt et al 2000). As expected for this inert molecule, no hints at a metabolism were found.…”

Section: Feeding Of 3-fdg To a Abbreviatussupporting

confidence: 69%

In vivo19F NMR chemical-shift imaging ofAncistrocladus species

et al. 2001

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19F nuclear magnetic resonance (NMR) imaging and 19F NMR chemical-shift imaging (19F CSI) have been used to localize fluorinated compounds administered to stems of Ancistrocladus heyneanus and A. abbreviatus for the elucidation of biosynthetic pathways in living plants. This first application of 19F CSI on plants proved CSI to be a valuable technique for mapping fluorinated molecules in plants. Exemplarily using trifluoroacetate as a model compound allowed to select appropriate feeding methods and to optimize both concentration and duration of the application to the plant. The time course of the uptake and distribution of trifluoroacetate was monitored by both 19F imaging and 19F CSI. Fluorinated metabolites formed by uptake of 3-fluoro-3-deoxy-D-glucose were detected with 19F CSI.

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Section: Feeding Of 3-fdg To a Abbreviatussupporting

confidence: 69%

In vivo19F NMR chemical-shift imaging ofAncistrocladus species

et al. 2001

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“…Also Rokitta et al (1999) and Peuke et al (2001) determined xylem and phloem fluxes as a response of illumination by means of MRI (). Salt stress is topic of 23 Na MRI, which was applied for the first time by Olt et al (2000) Jeschke et al (1996) developed a partition model for the description of water, nitrogen, and carbon fluxes in R. communis Water and phosphorous stress. as well as excess water, reduce both transpiration and growth (Jeschke et al, 1996; Schurr et al, 2000; Else et al, 2001).…”

Section: Methodsmentioning

confidence: 99%

Changes in Soil Water Content Resulting fromRicinusRoot Uptake Monitored by Magnetic Resonance Imaging

Pohlmeier

Oros‐Peusquens

Javaux

et al. 2008

Vadose Zone Journal

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Magnetic resonance imaging (MRI) was used to study the soil water content changes caused by root water uptake. A 4‐wk‐old Ricinus communis carmencita with a highly developed root system was planted in a cylindrical container filled with a model soil (99.5% fine sand, 0.5% clay), fully water saturated. The bottom and surface of the container were sealed so that water loss by factors other than transpiration via the leaves could be neglected. The water content of the soil was monitored for 3 wk using the MRI sequence SPRITE at an isotropic spatial resolution of 6.3 mm. In contrast to conventionally used MRI sequences, the T2* relaxation was monitored, temporally resolved, and extrapolated to zero. This procedure is a better measure of water content than a single signal at a given time point since it eliminates varying MRI relaxation times during soil desiccation. A linear correlation between the MRI‐determined and gravimetrically measured total water content proves the correctness of the monitoring and data evaluation procedure. Simultaneously, the root architecture was also imaged at 0.6 mm isotropic resolution by the MRI sequence constructive interference in steady state (CISS), which yielded a good contrast between soil and roots. The coregistration of both types of imaging (water content and root architecture) indicates that greater changes in water content took place in the bottom region and near the surface, where the highest root densities were found.

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“…In this category, the benefits of microelectrode measurements for studying of single cell metabolism have been advocated . Ion activities in living plant cells are recorded by patch-clamp techniques , fluorescent dyes Läuchli, 2002b, Halperin and and NMR technologies (Olt et al, 2000, Gruwel et al, 2001, whereas X-ray microanalysis offers an opportunity for quantifying the amount of an element (Flowers and Hajibagheri, 2001). The studies of nutrient status and transport on the microscale rely on other methodologies as well, such as: the kinematic growth analysis and elemental deposition rates, microdissection, specimen preparations, secondary ion mass spectrometry, and other microanalytical techniques, like microautoradiography (Lazof and Bernstain, 1999).…”

Section: Screening Of Plant Responses To Salinity Current Techniquesmentioning

confidence: 99%

Salt Stress

DAJIC

2006

Physiology and Molecular Biology of Stress Tolerance in Plants

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23Na and 1H NMR Microimaging of Intact Plants

Cited by 24 publications

References 21 publications

In vivo19F NMR chemical-shift imaging ofAncistrocladus species

In vivo19F NMR chemical-shift imaging ofAncistrocladus species

Changes in Soil Water Content Resulting fromRicinusRoot Uptake Monitored by Magnetic Resonance Imaging

Salt Stress

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